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Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin

Background: Postoperative chronic pain caused by fixation and/or fibrotic incorporation of hernia meshes are the main concerns in inguinal herniorrhaphy. As inguinal hernia is a degenerative disease, logically the treatment should aim at stopping degeneration and activating regeneration. Unfortunate...

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Autores principales: Amato, Giuseppe, Romano, Giorgio, Rodolico, Vito, Puleio, Roberto, Calò, Pietro Giorgio, Di Buono, Giuseppe, Cicero, Luca, Goetze, Thorsten Oliver, Agrusa, Antonino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680268/
https://www.ncbi.nlm.nih.gov/pubmed/36412894
http://dx.doi.org/10.3390/jfb13040253
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author Amato, Giuseppe
Romano, Giorgio
Rodolico, Vito
Puleio, Roberto
Calò, Pietro Giorgio
Di Buono, Giuseppe
Cicero, Luca
Romano, Giorgio
Goetze, Thorsten Oliver
Agrusa, Antonino
author_facet Amato, Giuseppe
Romano, Giorgio
Rodolico, Vito
Puleio, Roberto
Calò, Pietro Giorgio
Di Buono, Giuseppe
Cicero, Luca
Romano, Giorgio
Goetze, Thorsten Oliver
Agrusa, Antonino
author_sort Amato, Giuseppe
collection PubMed
description Background: Postoperative chronic pain caused by fixation and/or fibrotic incorporation of hernia meshes are the main concerns in inguinal herniorrhaphy. As inguinal hernia is a degenerative disease, logically the treatment should aim at stopping degeneration and activating regeneration. Unfortunately, in conventional prosthetic herniorrhaphy no relationship exists between pathogenesis and treatment. To overcome these incongruences, a 3D dynamic responsive multilamellar scaffold has been developed for fixation-free inguinal hernia repair. Made of polypropylene like conventional flat meshes, the dynamic behavior of the scaffold allows for the regeneration of all typical inguinal components: connective tissue, vessels, nerves, and myocytes. This investigation aims to demonstrate that, moving in tune with the groin, the 3D scaffold attracts myogenic growth factors activating the development of mature myocytes and, thus, re-establishing the herniated inguinal barrier. Methods: Biopsy samples excised from the 3D scaffold at different postoperative stages were stained with H&E and Azan–Mallory; immunohistochemistry for NGF and NGFR p75 was performed to verify the degree of involvement of muscular growth factors in the neomyogenesis. Results: Histological evidence of progressive muscle development and immunohistochemical proof of NFG and NFGRp75 contribution in neomyogenesis within the 3D scaffold was documented and statistically validated. Conclusion: The investigation appears to confirm that a 3D polypropylene scaffold designed to confer dynamic responsivity, unlike the fibrotic scar plate of static meshes, attracts myogenic growth factors turning the biological response into tissue regeneration. Newly developed muscles allow the scaffold to restore the integrity of the inguinal barrier.
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spelling pubmed-96802682022-11-23 Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin Amato, Giuseppe Romano, Giorgio Rodolico, Vito Puleio, Roberto Calò, Pietro Giorgio Di Buono, Giuseppe Cicero, Luca Romano, Giorgio Goetze, Thorsten Oliver Agrusa, Antonino J Funct Biomater Article Background: Postoperative chronic pain caused by fixation and/or fibrotic incorporation of hernia meshes are the main concerns in inguinal herniorrhaphy. As inguinal hernia is a degenerative disease, logically the treatment should aim at stopping degeneration and activating regeneration. Unfortunately, in conventional prosthetic herniorrhaphy no relationship exists between pathogenesis and treatment. To overcome these incongruences, a 3D dynamic responsive multilamellar scaffold has been developed for fixation-free inguinal hernia repair. Made of polypropylene like conventional flat meshes, the dynamic behavior of the scaffold allows for the regeneration of all typical inguinal components: connective tissue, vessels, nerves, and myocytes. This investigation aims to demonstrate that, moving in tune with the groin, the 3D scaffold attracts myogenic growth factors activating the development of mature myocytes and, thus, re-establishing the herniated inguinal barrier. Methods: Biopsy samples excised from the 3D scaffold at different postoperative stages were stained with H&E and Azan–Mallory; immunohistochemistry for NGF and NGFR p75 was performed to verify the degree of involvement of muscular growth factors in the neomyogenesis. Results: Histological evidence of progressive muscle development and immunohistochemical proof of NFG and NFGRp75 contribution in neomyogenesis within the 3D scaffold was documented and statistically validated. Conclusion: The investigation appears to confirm that a 3D polypropylene scaffold designed to confer dynamic responsivity, unlike the fibrotic scar plate of static meshes, attracts myogenic growth factors turning the biological response into tissue regeneration. Newly developed muscles allow the scaffold to restore the integrity of the inguinal barrier. MDPI 2022-11-18 /pmc/articles/PMC9680268/ /pubmed/36412894 http://dx.doi.org/10.3390/jfb13040253 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amato, Giuseppe
Romano, Giorgio
Rodolico, Vito
Puleio, Roberto
Calò, Pietro Giorgio
Di Buono, Giuseppe
Cicero, Luca
Romano, Giorgio
Goetze, Thorsten Oliver
Agrusa, Antonino
Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title_full Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title_fullStr Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title_full_unstemmed Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title_short Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin
title_sort dynamic responsive inguinal scaffold activates myogenic growth factors finalizing the regeneration of the herniated groin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680268/
https://www.ncbi.nlm.nih.gov/pubmed/36412894
http://dx.doi.org/10.3390/jfb13040253
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