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Optimized Lambda Exonuclease Digestion or Purification Using Streptavidin-Coated Beads: Which One Is Best for Successful DNA Aptamer Selection?

The high failure rate of the in vitro aptamer selection process by SELEX (Systematic Evolution of Ligands by EXponential enrichment) limits the production of these innovative oligonucleotides and, consequently, limits their potential applications. The generation of single-stranded DNA (ssDNA) is a c...

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Detalles Bibliográficos
Autores principales:	Le Dortz, Lisa Lucie, Rouxel, Clotilde, Leroy, Quentin, Brosseau, Noah, Boulouis, Henri-Jean, Haddad, Nadia, Lagrée, Anne-Claire, Deshuillers, Pierre Lucien
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680285/ https://www.ncbi.nlm.nih.gov/pubmed/36412811 http://dx.doi.org/10.3390/mps5060089

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