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Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynth...

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Autores principales: Hashem, Amr H., Saied, Ebrahim, Amin, Basma H., Alotibi, Fatimah O., Al-Askar, Abdulaziz A., Arishi, Amr A., Elkady, Fathy M., Elbahnasawy, Mostafa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680418/
https://www.ncbi.nlm.nih.gov/pubmed/36412883
http://dx.doi.org/10.3390/jfb13040242
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author Hashem, Amr H.
Saied, Ebrahim
Amin, Basma H.
Alotibi, Fatimah O.
Al-Askar, Abdulaziz A.
Arishi, Amr A.
Elkady, Fathy M.
Elbahnasawy, Mostafa A.
author_facet Hashem, Amr H.
Saied, Ebrahim
Amin, Basma H.
Alotibi, Fatimah O.
Al-Askar, Abdulaziz A.
Arishi, Amr A.
Elkady, Fathy M.
Elbahnasawy, Mostafa A.
author_sort Hashem, Amr H.
collection PubMed
description Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC(50) = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.
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spelling pubmed-96804182022-11-23 Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study Hashem, Amr H. Saied, Ebrahim Amin, Basma H. Alotibi, Fatimah O. Al-Askar, Abdulaziz A. Arishi, Amr A. Elkady, Fathy M. Elbahnasawy, Mostafa A. J Funct Biomater Article Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC(50) = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis. MDPI 2022-11-15 /pmc/articles/PMC9680418/ /pubmed/36412883 http://dx.doi.org/10.3390/jfb13040242 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hashem, Amr H.
Saied, Ebrahim
Amin, Basma H.
Alotibi, Fatimah O.
Al-Askar, Abdulaziz A.
Arishi, Amr A.
Elkady, Fathy M.
Elbahnasawy, Mostafa A.
Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title_full Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title_fullStr Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title_full_unstemmed Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title_short Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study
title_sort antifungal activity of biosynthesized silver nanoparticles (agnps) against aspergilli causing aspergillosis: ultrastructure study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680418/
https://www.ncbi.nlm.nih.gov/pubmed/36412883
http://dx.doi.org/10.3390/jfb13040242
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