Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase

We present an efficient approach for tag-free, site-specific conjugation of a fully glycosylated antibody using microbial transglutaminase (mTG). We created variants of trastuzumab where a single surface-exposed residue of the human crystallizable fragment had been substituted to glutamine, with the...

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Detalles Bibliográficos
Autores principales: Hadjabdelhafid-Parisien, Adem, Bitsch, Sebastian, Macarrón Palacios, Arturo, Deweid, Lukas, Kolmar, Harald, Pelletier, Joelle N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680618/
https://www.ncbi.nlm.nih.gov/pubmed/36505706
http://dx.doi.org/10.1039/d2ra05630e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680618/
https://www.ncbi.nlm.nih.gov/pubmed/36505706
http://dx.doi.org/10.1039/d2ra05630e

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