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Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm
The closing of the gated ion channel in the cystic fibrosis transmembrane conductance regulator can be categorized as nonpermissive to reopening, which involves the unbinding of ADP or ATP, or permissive, which does not. Identifying the type of closing is of interest as interactions with nucleotides...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680790/ https://www.ncbi.nlm.nih.gov/pubmed/36425670 http://dx.doi.org/10.1016/j.bpr.2022.100083 |
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author | Moffett, Alexander S. Cui, Guiying Thomas, Peter J. Hunt, William D. McCarty, Nael A. Westafer, Ryan S. Eckford, Andrew W. |
author_facet | Moffett, Alexander S. Cui, Guiying Thomas, Peter J. Hunt, William D. McCarty, Nael A. Westafer, Ryan S. Eckford, Andrew W. |
author_sort | Moffett, Alexander S. |
collection | PubMed |
description | The closing of the gated ion channel in the cystic fibrosis transmembrane conductance regulator can be categorized as nonpermissive to reopening, which involves the unbinding of ADP or ATP, or permissive, which does not. Identifying the type of closing is of interest as interactions with nucleotides can be affected in mutants or by introducing agonists. However, all closings are electrically silent and difficult to differentiate. For single-channel patch-clamp traces, we show that the type of the closing can be accurately determined by an inference algorithm implemented on a factor graph, which we demonstrate using both simulated and lab-obtained patch-clamp traces. |
format | Online Article Text |
id | pubmed-9680790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96807902022-11-23 Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm Moffett, Alexander S. Cui, Guiying Thomas, Peter J. Hunt, William D. McCarty, Nael A. Westafer, Ryan S. Eckford, Andrew W. Biophys Rep (N Y) Report The closing of the gated ion channel in the cystic fibrosis transmembrane conductance regulator can be categorized as nonpermissive to reopening, which involves the unbinding of ADP or ATP, or permissive, which does not. Identifying the type of closing is of interest as interactions with nucleotides can be affected in mutants or by introducing agonists. However, all closings are electrically silent and difficult to differentiate. For single-channel patch-clamp traces, we show that the type of the closing can be accurately determined by an inference algorithm implemented on a factor graph, which we demonstrate using both simulated and lab-obtained patch-clamp traces. Elsevier 2022-10-19 /pmc/articles/PMC9680790/ /pubmed/36425670 http://dx.doi.org/10.1016/j.bpr.2022.100083 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Report Moffett, Alexander S. Cui, Guiying Thomas, Peter J. Hunt, William D. McCarty, Nael A. Westafer, Ryan S. Eckford, Andrew W. Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title | Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title_full | Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title_fullStr | Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title_full_unstemmed | Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title_short | Permissive and nonpermissive channel closings in CFTR revealed by a factor graph inference algorithm |
title_sort | permissive and nonpermissive channel closings in cftr revealed by a factor graph inference algorithm |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680790/ https://www.ncbi.nlm.nih.gov/pubmed/36425670 http://dx.doi.org/10.1016/j.bpr.2022.100083 |
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