Cargando…

BIPS—A code base for designing and coding of a Phage ImmunoPrecipitation Oligo Library

BIPS (Build Phage ImmunoPrecipitation Sequencing library) is a software that converts a list of proteins into a custom DNA oligonucleotide library for the PhIP-Seq system. The tool creates constant-length oligonucleotides with internal barcodes, while maintaining the original length of the peptide....

Descripción completa

Detalles Bibliográficos
Autores principales: Leviatan, Sigal, Kalka, Iris N., Vogl, Thomas, Klompas, Shelley, Weinberger, Adina, Segal, Eran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681064/
https://www.ncbi.nlm.nih.gov/pubmed/36355866
http://dx.doi.org/10.1371/journal.pcbi.1010663
Descripción
Sumario:BIPS (Build Phage ImmunoPrecipitation Sequencing library) is a software that converts a list of proteins into a custom DNA oligonucleotide library for the PhIP-Seq system. The tool creates constant-length oligonucleotides with internal barcodes, while maintaining the original length of the peptide. This allows using large libraries, of hundreds of thousands of oligonucleotides, while saving on the costs of sequencing and maintaining the accuracy of oligonucleotide reads identification. BIPS is available under GNU public license from: https://github.com/kalkairis/BuildPhIPSeqLibrary.