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MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis
Histone acetylation has been shown to involve in stress responses. However, the detailed molecular mechanisms that how histone deacetylases and transcription factors function in drought stress response remain to be understood. In this research, we show that ENAP1 and ENAP2 are positive regulators of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681084/ https://www.ncbi.nlm.nih.gov/pubmed/36413574 http://dx.doi.org/10.1371/journal.pgen.1010473 |
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author | Zhao, Bo Shao, Zhengyao Wang, Likai Zhang, Fan Chakravarty, Daveraj Zong, Wei Dong, Juan Song, Liang Qiao, Hong |
author_facet | Zhao, Bo Shao, Zhengyao Wang, Likai Zhang, Fan Chakravarty, Daveraj Zong, Wei Dong, Juan Song, Liang Qiao, Hong |
author_sort | Zhao, Bo |
collection | PubMed |
description | Histone acetylation has been shown to involve in stress responses. However, the detailed molecular mechanisms that how histone deacetylases and transcription factors function in drought stress response remain to be understood. In this research, we show that ENAP1 and ENAP2 are positive regulators of drought tolerance in plants, and the enap1enap2 double mutant is more sensitive to drought stress. Both ENAP1 and ENAP2 interact with MYB44, a transcription factor that interacts with histone deacetylase HDT4. Genetics data show that myb44 null mutation enhances the sensitivity of enap1enap2 to drought stress. Whereas, HDT4 negatively regulates plant drought response, the hdt4 mutant represses enap1enap2myb44 drought sensitive phenotype. In the normal condition, ENAP1/2 and MYB44 counteract the HDT4 function for the regulation of H3K27ac. Upon drought stress, the accumulation of MYB44 and reduction of HDT4 leads to the enrichment of H3K27ac and the activation of target gene expression. Overall, this research provides a novel molecular mechanism by which ENAP1, ENAP2 and MYB44 form a complex to restrict the function of HDT4 in the normal condition; under drought condition, accumulated MYB44 and reduced HDT4 lead to the elevation of H3K27ac and the expression of drought responsive genes, as a result, plants are drought tolerant. |
format | Online Article Text |
id | pubmed-9681084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96810842022-11-23 MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis Zhao, Bo Shao, Zhengyao Wang, Likai Zhang, Fan Chakravarty, Daveraj Zong, Wei Dong, Juan Song, Liang Qiao, Hong PLoS Genet Research Article Histone acetylation has been shown to involve in stress responses. However, the detailed molecular mechanisms that how histone deacetylases and transcription factors function in drought stress response remain to be understood. In this research, we show that ENAP1 and ENAP2 are positive regulators of drought tolerance in plants, and the enap1enap2 double mutant is more sensitive to drought stress. Both ENAP1 and ENAP2 interact with MYB44, a transcription factor that interacts with histone deacetylase HDT4. Genetics data show that myb44 null mutation enhances the sensitivity of enap1enap2 to drought stress. Whereas, HDT4 negatively regulates plant drought response, the hdt4 mutant represses enap1enap2myb44 drought sensitive phenotype. In the normal condition, ENAP1/2 and MYB44 counteract the HDT4 function for the regulation of H3K27ac. Upon drought stress, the accumulation of MYB44 and reduction of HDT4 leads to the enrichment of H3K27ac and the activation of target gene expression. Overall, this research provides a novel molecular mechanism by which ENAP1, ENAP2 and MYB44 form a complex to restrict the function of HDT4 in the normal condition; under drought condition, accumulated MYB44 and reduced HDT4 lead to the elevation of H3K27ac and the expression of drought responsive genes, as a result, plants are drought tolerant. Public Library of Science 2022-11-22 /pmc/articles/PMC9681084/ /pubmed/36413574 http://dx.doi.org/10.1371/journal.pgen.1010473 Text en © 2022 Zhao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhao, Bo Shao, Zhengyao Wang, Likai Zhang, Fan Chakravarty, Daveraj Zong, Wei Dong, Juan Song, Liang Qiao, Hong MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title | MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title_full | MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title_fullStr | MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title_full_unstemmed | MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title_short | MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis |
title_sort | myb44-enap1/2 restricts hdt4 to regulate drought tolerance in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681084/ https://www.ncbi.nlm.nih.gov/pubmed/36413574 http://dx.doi.org/10.1371/journal.pgen.1010473 |
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