Cargando…

Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis

Recent evidence demonstrated that functional bacteria were involved in the regulation of Parkinson’s disease (PD). However, the mechanism of probiotics in improving PD was unclear. Here the antioxidant effect and the mechanism of probiotics Pediococcus pentosaceus (PP) on PD were studied by regulati...

Descripción completa

Detalles Bibliográficos
Autores principales: Pan, Sipei, Wei, Hongming, Yuan, Shushu, Kong, Yu, Yang, Huiqun, Zhang, Yuhe, Cui, Xiaorui, Chen, Weian, Liu, Jiaming, Zhang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682001/
https://www.ncbi.nlm.nih.gov/pubmed/36439235
http://dx.doi.org/10.3389/fcimb.2022.1022879
_version_ 1784834749375709184
author Pan, Sipei
Wei, Hongming
Yuan, Shushu
Kong, Yu
Yang, Huiqun
Zhang, Yuhe
Cui, Xiaorui
Chen, Weian
Liu, Jiaming
Zhang, Yang
author_facet Pan, Sipei
Wei, Hongming
Yuan, Shushu
Kong, Yu
Yang, Huiqun
Zhang, Yuhe
Cui, Xiaorui
Chen, Weian
Liu, Jiaming
Zhang, Yang
author_sort Pan, Sipei
collection PubMed
description Recent evidence demonstrated that functional bacteria were involved in the regulation of Parkinson’s disease (PD). However, the mechanism of probiotics in improving PD was unclear. Here the antioxidant effect and the mechanism of probiotics Pediococcus pentosaceus (PP) on PD were studied by regulating the gut–brain axis. In this study, male C57BL/6J mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneally to establish a PD model and were then treated with PP for 4 weeks. Subsequently, a series of neurobehavioral tests to evaluate the motor function of the mice was performed. Additionally, degeneration of dopaminergic neurons, accumulation of α-synuclein, the production of an oxidative stress response, and the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related proteins were evaluated. Moreover, the gut microbial composition and the level of metabolite γ-aminobutyric acid (GABA) were assessed. The results showed that PP treatment could improve MPTP-induced motor deficits, the degeneration of dopaminergic neurons, and the accumulation of α-synuclein. Moreover, PP treatment significantly increased the levels of SOD1, Gpx1, and Nrf2, while it decreased the levels of Keap1 in the brain of MPTP-induced mice. Notably, PP treatment improved the gut microbial dysbiosis and increased the level of GABA in MPTP-induced mice. These findings indicated that PP might represent a promising candidate, due to the metabolite of GABA, that could be used for the treatment of PD.
format Online
Article
Text
id pubmed-9682001
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96820012022-11-24 Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis Pan, Sipei Wei, Hongming Yuan, Shushu Kong, Yu Yang, Huiqun Zhang, Yuhe Cui, Xiaorui Chen, Weian Liu, Jiaming Zhang, Yang Front Cell Infect Microbiol Cellular and Infection Microbiology Recent evidence demonstrated that functional bacteria were involved in the regulation of Parkinson’s disease (PD). However, the mechanism of probiotics in improving PD was unclear. Here the antioxidant effect and the mechanism of probiotics Pediococcus pentosaceus (PP) on PD were studied by regulating the gut–brain axis. In this study, male C57BL/6J mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneally to establish a PD model and were then treated with PP for 4 weeks. Subsequently, a series of neurobehavioral tests to evaluate the motor function of the mice was performed. Additionally, degeneration of dopaminergic neurons, accumulation of α-synuclein, the production of an oxidative stress response, and the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related proteins were evaluated. Moreover, the gut microbial composition and the level of metabolite γ-aminobutyric acid (GABA) were assessed. The results showed that PP treatment could improve MPTP-induced motor deficits, the degeneration of dopaminergic neurons, and the accumulation of α-synuclein. Moreover, PP treatment significantly increased the levels of SOD1, Gpx1, and Nrf2, while it decreased the levels of Keap1 in the brain of MPTP-induced mice. Notably, PP treatment improved the gut microbial dysbiosis and increased the level of GABA in MPTP-induced mice. These findings indicated that PP might represent a promising candidate, due to the metabolite of GABA, that could be used for the treatment of PD. Frontiers Media S.A. 2022-11-09 /pmc/articles/PMC9682001/ /pubmed/36439235 http://dx.doi.org/10.3389/fcimb.2022.1022879 Text en Copyright © 2022 Pan, Wei, Yuan, Kong, Yang, Zhang, Cui, Chen, Liu and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Pan, Sipei
Wei, Hongming
Yuan, Shushu
Kong, Yu
Yang, Huiqun
Zhang, Yuhe
Cui, Xiaorui
Chen, Weian
Liu, Jiaming
Zhang, Yang
Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title_full Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title_fullStr Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title_full_unstemmed Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title_short Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis
title_sort probiotic pediococcus pentosaceus ameliorates mptp-induced oxidative stress via regulating the gut microbiota–gut–brain axis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682001/
https://www.ncbi.nlm.nih.gov/pubmed/36439235
http://dx.doi.org/10.3389/fcimb.2022.1022879
work_keys_str_mv AT pansipei probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT weihongming probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT yuanshushu probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT kongyu probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT yanghuiqun probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT zhangyuhe probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT cuixiaorui probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT chenweian probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT liujiaming probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis
AT zhangyang probioticpediococcuspentosaceusamelioratesmptpinducedoxidativestressviaregulatingthegutmicrobiotagutbrainaxis