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Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and sign...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682043/ https://www.ncbi.nlm.nih.gov/pubmed/36438054 http://dx.doi.org/10.3389/fmed.2022.1045990 |
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author | Jing, Dalan Jiang, Xiaodan Ren, Xiaotong Su, Jie Huang, Chen Yang, Jiarui Hao, Ran Li, Xuemin |
author_facet | Jing, Dalan Jiang, Xiaodan Ren, Xiaotong Su, Jie Huang, Chen Yang, Jiarui Hao, Ran Li, Xuemin |
author_sort | Jing, Dalan |
collection | PubMed |
description | PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing. RESULTS: The positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture. CONCLUSION: The CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms. |
format | Online Article Text |
id | pubmed-9682043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96820432022-11-24 Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction Jing, Dalan Jiang, Xiaodan Ren, Xiaotong Su, Jie Huang, Chen Yang, Jiarui Hao, Ran Li, Xuemin Front Med (Lausanne) Medicine PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing. RESULTS: The positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture. CONCLUSION: The CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms. Frontiers Media S.A. 2022-11-09 /pmc/articles/PMC9682043/ /pubmed/36438054 http://dx.doi.org/10.3389/fmed.2022.1045990 Text en Copyright © 2022 Jing, Jiang, Ren, Su, Huang, Yang, Hao and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Jing, Dalan Jiang, Xiaodan Ren, Xiaotong Su, Jie Huang, Chen Yang, Jiarui Hao, Ran Li, Xuemin Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title_full | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title_fullStr | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title_full_unstemmed | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title_short | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
title_sort | metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682043/ https://www.ncbi.nlm.nih.gov/pubmed/36438054 http://dx.doi.org/10.3389/fmed.2022.1045990 |
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