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Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction

PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and sign...

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Detalles Bibliográficos
Autores principales: Jing, Dalan, Jiang, Xiaodan, Ren, Xiaotong, Su, Jie, Huang, Chen, Yang, Jiarui, Hao, Ran, Li, Xuemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682043/
https://www.ncbi.nlm.nih.gov/pubmed/36438054
http://dx.doi.org/10.3389/fmed.2022.1045990
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author Jing, Dalan
Jiang, Xiaodan
Ren, Xiaotong
Su, Jie
Huang, Chen
Yang, Jiarui
Hao, Ran
Li, Xuemin
author_facet Jing, Dalan
Jiang, Xiaodan
Ren, Xiaotong
Su, Jie
Huang, Chen
Yang, Jiarui
Hao, Ran
Li, Xuemin
author_sort Jing, Dalan
collection PubMed
description PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing. RESULTS: The positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture. CONCLUSION: The CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms.
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spelling pubmed-96820432022-11-24 Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction Jing, Dalan Jiang, Xiaodan Ren, Xiaotong Su, Jie Huang, Chen Yang, Jiarui Hao, Ran Li, Xuemin Front Med (Lausanne) Medicine PURPOSE: To explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing. METHODS: A total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing. RESULTS: The positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture. CONCLUSION: The CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms. Frontiers Media S.A. 2022-11-09 /pmc/articles/PMC9682043/ /pubmed/36438054 http://dx.doi.org/10.3389/fmed.2022.1045990 Text en Copyright © 2022 Jing, Jiang, Ren, Su, Huang, Yang, Hao and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Jing, Dalan
Jiang, Xiaodan
Ren, Xiaotong
Su, Jie
Huang, Chen
Yang, Jiarui
Hao, Ran
Li, Xuemin
Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title_full Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title_fullStr Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title_full_unstemmed Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title_short Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
title_sort metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682043/
https://www.ncbi.nlm.nih.gov/pubmed/36438054
http://dx.doi.org/10.3389/fmed.2022.1045990
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