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The impact of dietary fibers on Clostridioides difficile infection in a mouse model

Diets rich in fiber may provide health benefits and regulate the gut microbiome, which affects the immune system. However, the role of dietary fiber in Clostridioides difficile infection (CDI) is controversial. Here, we investigated the use of fermentable fibers, such as inulin or pectin, to replace...

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Autores principales: Wu, Zhengjie, Xu, Qiaomai, Wang, Qiangqiang, Chen, Yunbo, Lv, Longxian, Zheng, Beiwen, Yan, Ren, Jiang, Huiyong, Shen, Jian, Wang, Shuting, Wang, Kaicen, Xia, Jiafeng, Han, Shengyi, Li, Lanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682084/
https://www.ncbi.nlm.nih.gov/pubmed/36439215
http://dx.doi.org/10.3389/fcimb.2022.1028267
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author Wu, Zhengjie
Xu, Qiaomai
Wang, Qiangqiang
Chen, Yunbo
Lv, Longxian
Zheng, Beiwen
Yan, Ren
Jiang, Huiyong
Shen, Jian
Wang, Shuting
Wang, Kaicen
Xia, Jiafeng
Han, Shengyi
Li, Lanjuan
author_facet Wu, Zhengjie
Xu, Qiaomai
Wang, Qiangqiang
Chen, Yunbo
Lv, Longxian
Zheng, Beiwen
Yan, Ren
Jiang, Huiyong
Shen, Jian
Wang, Shuting
Wang, Kaicen
Xia, Jiafeng
Han, Shengyi
Li, Lanjuan
author_sort Wu, Zhengjie
collection PubMed
description Diets rich in fiber may provide health benefits and regulate the gut microbiome, which affects the immune system. However, the role of dietary fiber in Clostridioides difficile infection (CDI) is controversial. Here, we investigated the use of fermentable fibers, such as inulin or pectin, to replace the insoluble fiber cellulose to explore how dietary fiber affects C. difficile-induced colitis in mice through intestinal microecology and metabolomics. Using C. difficile VPI 10463, we generated a mouse model of antibiotic-induced CDI. We evaluated disease outcomes and the microbial community among mice fed two fermentable fibers (inulin or pectin) versus the insoluble fiber cellulose. We analyzed and compared the gut microbiota, intestinal epithelium, cytokine levels, immune responses, and metabolites between the groups. Severe histological injury and elevated cytokine levels were observed in colon tissues after infection. Different diets showed different effects, and pectin administration protected intestinal epithelial permeability. Pectin also steadily increased the diversity of the microbiome and decreased the levels of C. difficile-induced markers of inflammation in serum and colonic tissues. The pectin group showed a higher abundance of Lachnospiraceae and a lower abundance of the conditionally pathogenic Enterobacteriaceae than the cellulose group with infection. The concentration of short-chain fatty acids in the cecal contents was also higher in the pectin group than in the cellulose group. Pectin exerted its effects through the aryl hydrocarbon receptor (AhR) pathway, which was confirmed by using the AhR agonist FICZ and the inhibitor CH2223191. Our results show that pectin alters the microbiome and metabolic function and triggers a protective immune response.
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spelling pubmed-96820842022-11-24 The impact of dietary fibers on Clostridioides difficile infection in a mouse model Wu, Zhengjie Xu, Qiaomai Wang, Qiangqiang Chen, Yunbo Lv, Longxian Zheng, Beiwen Yan, Ren Jiang, Huiyong Shen, Jian Wang, Shuting Wang, Kaicen Xia, Jiafeng Han, Shengyi Li, Lanjuan Front Cell Infect Microbiol Cellular and Infection Microbiology Diets rich in fiber may provide health benefits and regulate the gut microbiome, which affects the immune system. However, the role of dietary fiber in Clostridioides difficile infection (CDI) is controversial. Here, we investigated the use of fermentable fibers, such as inulin or pectin, to replace the insoluble fiber cellulose to explore how dietary fiber affects C. difficile-induced colitis in mice through intestinal microecology and metabolomics. Using C. difficile VPI 10463, we generated a mouse model of antibiotic-induced CDI. We evaluated disease outcomes and the microbial community among mice fed two fermentable fibers (inulin or pectin) versus the insoluble fiber cellulose. We analyzed and compared the gut microbiota, intestinal epithelium, cytokine levels, immune responses, and metabolites between the groups. Severe histological injury and elevated cytokine levels were observed in colon tissues after infection. Different diets showed different effects, and pectin administration protected intestinal epithelial permeability. Pectin also steadily increased the diversity of the microbiome and decreased the levels of C. difficile-induced markers of inflammation in serum and colonic tissues. The pectin group showed a higher abundance of Lachnospiraceae and a lower abundance of the conditionally pathogenic Enterobacteriaceae than the cellulose group with infection. The concentration of short-chain fatty acids in the cecal contents was also higher in the pectin group than in the cellulose group. Pectin exerted its effects through the aryl hydrocarbon receptor (AhR) pathway, which was confirmed by using the AhR agonist FICZ and the inhibitor CH2223191. Our results show that pectin alters the microbiome and metabolic function and triggers a protective immune response. Frontiers Media S.A. 2022-11-09 /pmc/articles/PMC9682084/ /pubmed/36439215 http://dx.doi.org/10.3389/fcimb.2022.1028267 Text en Copyright © 2022 Wu, Xu, Wang, Chen, Lv, Zheng, Yan, Jiang, Shen, Wang, Wang, Xia, Han and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Wu, Zhengjie
Xu, Qiaomai
Wang, Qiangqiang
Chen, Yunbo
Lv, Longxian
Zheng, Beiwen
Yan, Ren
Jiang, Huiyong
Shen, Jian
Wang, Shuting
Wang, Kaicen
Xia, Jiafeng
Han, Shengyi
Li, Lanjuan
The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title_full The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title_fullStr The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title_full_unstemmed The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title_short The impact of dietary fibers on Clostridioides difficile infection in a mouse model
title_sort impact of dietary fibers on clostridioides difficile infection in a mouse model
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682084/
https://www.ncbi.nlm.nih.gov/pubmed/36439215
http://dx.doi.org/10.3389/fcimb.2022.1028267
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