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Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review
Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A sequences, IRES, or intein. Nevertheless, each o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Tehran University of Medical Sciences
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682371/ https://www.ncbi.nlm.nih.gov/pubmed/36466018 http://dx.doi.org/10.18502/ijpa.v17i3.10618 |
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author | Abdi Ghavidel, Afshin Jajarmi, Vahid Bandehpour, Mojgan Kazemi, Bahram |
author_facet | Abdi Ghavidel, Afshin Jajarmi, Vahid Bandehpour, Mojgan Kazemi, Bahram |
author_sort | Abdi Ghavidel, Afshin |
collection | PubMed |
description | Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A sequences, IRES, or intein. Nevertheless, each of these elements has several disadvantages that limit their usage. In this article, we suggest a new system for expressing multi-subunit proteins, which have several advantages over existing methods meanwhile it, lacks most of their disadvantages. Leishmania is a unicellular eukaryote and member of the Trypanosomatidae family. In the expression system of Leishmania, pre-long RNAs that contain several protein sequences transcribe. Then these long RNAs separate into mature mRNAs in the process named trans splicing. For producing multi-subunit protein, Leishmania transformed with a vector containing the sequences of all subunits. Therefore, those subunits translate and form the complex under eukaryotic cell conditions. The sequence of each protein must separate by the spatial sequence needed for trans splicing. Based on a Leishmania expression pattern, not only is it possible to produce the complexes with the correct structures and post-translational modifications, but also it is possible to overcome previous method problems. |
format | Online Article Text |
id | pubmed-9682371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-96823712022-12-02 Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review Abdi Ghavidel, Afshin Jajarmi, Vahid Bandehpour, Mojgan Kazemi, Bahram Iran J Parasitol Review Article Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A sequences, IRES, or intein. Nevertheless, each of these elements has several disadvantages that limit their usage. In this article, we suggest a new system for expressing multi-subunit proteins, which have several advantages over existing methods meanwhile it, lacks most of their disadvantages. Leishmania is a unicellular eukaryote and member of the Trypanosomatidae family. In the expression system of Leishmania, pre-long RNAs that contain several protein sequences transcribe. Then these long RNAs separate into mature mRNAs in the process named trans splicing. For producing multi-subunit protein, Leishmania transformed with a vector containing the sequences of all subunits. Therefore, those subunits translate and form the complex under eukaryotic cell conditions. The sequence of each protein must separate by the spatial sequence needed for trans splicing. Based on a Leishmania expression pattern, not only is it possible to produce the complexes with the correct structures and post-translational modifications, but also it is possible to overcome previous method problems. Tehran University of Medical Sciences 2022 /pmc/articles/PMC9682371/ /pubmed/36466018 http://dx.doi.org/10.18502/ijpa.v17i3.10618 Text en Copyright © 2022 Abdi Ghavidel et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Review Article Abdi Ghavidel, Afshin Jajarmi, Vahid Bandehpour, Mojgan Kazemi, Bahram Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title | Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title_full | Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title_fullStr | Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title_full_unstemmed | Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title_short | Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review |
title_sort | polycistronic expression of multi-subunit complexes in the eukaryotic environment: a narrative review |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9682371/ https://www.ncbi.nlm.nih.gov/pubmed/36466018 http://dx.doi.org/10.18502/ijpa.v17i3.10618 |
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