Cargando…
SARS-CoV-2 surveillance with environmental surface sampling in public areas
Contaminated surfaces are one of the ways that coronavirus disease 2019 (COVID-19) may be transmitted. SARS-CoV-2 can be detected on environmental surfaces; however, few environmental sampling studies have been conducted in nonclinical settings. The objective of this study was to detect SARS-CoV-2 R...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683569/ https://www.ncbi.nlm.nih.gov/pubmed/36417446 http://dx.doi.org/10.1371/journal.pone.0278061 |
_version_ | 1784835078643253248 |
---|---|
author | Mihajlovski, Kristina Buttner, Mark P. Cruz, Patricia Labus, Brian St. Pierre Schneider, Barbara Detrick, Elizabeth |
author_facet | Mihajlovski, Kristina Buttner, Mark P. Cruz, Patricia Labus, Brian St. Pierre Schneider, Barbara Detrick, Elizabeth |
author_sort | Mihajlovski, Kristina |
collection | PubMed |
description | Contaminated surfaces are one of the ways that coronavirus disease 2019 (COVID-19) may be transmitted. SARS-CoV-2 can be detected on environmental surfaces; however, few environmental sampling studies have been conducted in nonclinical settings. The objective of this study was to detect SARS-CoV-2 RNA on environmental surfaces in public areas in Las Vegas, Nevada. In total, 300 surface samples were collected from high-touch surfaces from high-congregate public locations and from a public health facility (PHF) that was visited by COVID-19 patients. Environmental samples were analyzed with quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) using SARS-CoV-2 specific primers and probes for three target genes. Results showed that 31 out of 300 (10.3%) surface samples tested positive for SARS-CoV-2, 24 at the PHF and 7 in high-congregate public locations. Concentrations ranged from 10(2) to 10(6) viral particles per 3 ml sample on a wide variety of materials. The data also showed that the N gene assay had greater sensitivity compared to the S and ORF gene assays. Besides frequently touched surfaces, SARS-CoV-2 was detected in restrooms, on floors and surfaces in contact with floors, as well as in a mop water sample. The results of this study describe the extent and distribution of environmental SARS-CoV-2 contamination in public areas in Las Vegas, Nevada. A method using the N gene PCR assay was developed for SARS-CoV-2 environmental monitoring in public areas. Environmental monitoring with this method can determine the specific sites of surface contamination in the community and may be beneficial for prevention of COVID-19 indirect transmission, and evaluation and improvement of infection control practices in public areas, public health facilities, universities, and businesses. |
format | Online Article Text |
id | pubmed-9683569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96835692022-11-24 SARS-CoV-2 surveillance with environmental surface sampling in public areas Mihajlovski, Kristina Buttner, Mark P. Cruz, Patricia Labus, Brian St. Pierre Schneider, Barbara Detrick, Elizabeth PLoS One Research Article Contaminated surfaces are one of the ways that coronavirus disease 2019 (COVID-19) may be transmitted. SARS-CoV-2 can be detected on environmental surfaces; however, few environmental sampling studies have been conducted in nonclinical settings. The objective of this study was to detect SARS-CoV-2 RNA on environmental surfaces in public areas in Las Vegas, Nevada. In total, 300 surface samples were collected from high-touch surfaces from high-congregate public locations and from a public health facility (PHF) that was visited by COVID-19 patients. Environmental samples were analyzed with quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) using SARS-CoV-2 specific primers and probes for three target genes. Results showed that 31 out of 300 (10.3%) surface samples tested positive for SARS-CoV-2, 24 at the PHF and 7 in high-congregate public locations. Concentrations ranged from 10(2) to 10(6) viral particles per 3 ml sample on a wide variety of materials. The data also showed that the N gene assay had greater sensitivity compared to the S and ORF gene assays. Besides frequently touched surfaces, SARS-CoV-2 was detected in restrooms, on floors and surfaces in contact with floors, as well as in a mop water sample. The results of this study describe the extent and distribution of environmental SARS-CoV-2 contamination in public areas in Las Vegas, Nevada. A method using the N gene PCR assay was developed for SARS-CoV-2 environmental monitoring in public areas. Environmental monitoring with this method can determine the specific sites of surface contamination in the community and may be beneficial for prevention of COVID-19 indirect transmission, and evaluation and improvement of infection control practices in public areas, public health facilities, universities, and businesses. Public Library of Science 2022-11-23 /pmc/articles/PMC9683569/ /pubmed/36417446 http://dx.doi.org/10.1371/journal.pone.0278061 Text en © 2022 Mihajlovski et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mihajlovski, Kristina Buttner, Mark P. Cruz, Patricia Labus, Brian St. Pierre Schneider, Barbara Detrick, Elizabeth SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title | SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title_full | SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title_fullStr | SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title_full_unstemmed | SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title_short | SARS-CoV-2 surveillance with environmental surface sampling in public areas |
title_sort | sars-cov-2 surveillance with environmental surface sampling in public areas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683569/ https://www.ncbi.nlm.nih.gov/pubmed/36417446 http://dx.doi.org/10.1371/journal.pone.0278061 |
work_keys_str_mv | AT mihajlovskikristina sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas AT buttnermarkp sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas AT cruzpatricia sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas AT labusbrian sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas AT stpierreschneiderbarbara sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas AT detrickelizabeth sarscov2surveillancewithenvironmentalsurfacesamplinginpublicareas |