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Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle

Percutaneous decompression of the cecum is a procedure that could be considered for horses with cecal gas distension. The aim of this study was to identify complications such as peritonitis and clinically relevant peritonitis (CRP) after transabdominal cecal trocarization in healthy horses using a c...

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Autores principales: Dória, Renata Gebara Sampaio, Reginato, Gustavo Morandini, Hayasaka, Yumi de Barcelos, Fantinato Neto, Paulo, Passarelli, Danielle, Arantes, Julia de Assis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683593/
https://www.ncbi.nlm.nih.gov/pubmed/36417417
http://dx.doi.org/10.1371/journal.pone.0277468
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author Dória, Renata Gebara Sampaio
Reginato, Gustavo Morandini
Hayasaka, Yumi de Barcelos
Fantinato Neto, Paulo
Passarelli, Danielle
Arantes, Julia de Assis
author_facet Dória, Renata Gebara Sampaio
Reginato, Gustavo Morandini
Hayasaka, Yumi de Barcelos
Fantinato Neto, Paulo
Passarelli, Danielle
Arantes, Julia de Assis
author_sort Dória, Renata Gebara Sampaio
collection PubMed
description Percutaneous decompression of the cecum is a procedure that could be considered for horses with cecal gas distension. The aim of this study was to identify complications such as peritonitis and clinically relevant peritonitis (CRP) after transabdominal cecal trocarization in healthy horses using a cattle trocar and a cecal needle. Mixed breed horses were assigned to three groups (n = 6): horses that underwent trocarization with a cecal needle (G1) or a cattle trocar (G2), and a control group (CG) without cecal trocarization. The same horses were used in each group, respecting a three-month washout period between studies. A physical examination, serial blood, and peritoneal fluid sampling were performed, prior to cecal trocarization and 2, 6 and 12 hours after the first collection and 1, 2, 3, 7, and 14 days after the procedure. Acute-phase proteins in blood and peritoneal fluid were analyzed by polyacrylamide gel electrophoresis. Horses with a high cell count in the peritoneal fluid (i.e., 10,000 cells/μl) were considered to have peritonitis and CRP if they met at least two of the following clinical criteria: anorexia, lethargy, tachycardia, tachypnea, fever, ileus, abnormal oral mucous membrane color, abnormal white blood cells count, or high blood fibrinogen concentration (> 5 g/L). All horses recovered from cecal trocarization and abdominocentesis with no major complications. Cecal trocarization caused cytologic evidence of peritonitis in G1 and G2 during the 14 days of evaluation. CRP was not observed, although a decrease in cecal motility was observed in G1 and G2 during the experimental period and three horses, one from G1 and two from G2, showed a single moment of fever. None of the groups showed leukopenia or leukocytosis, although blood neutrophil count decreased at D7 and D14 in G1 and at D14 in G2 (p ≤ 0.05). After cecal trocarization, an increase in the total nucleated cells count, total proteins, globulins, alkaline phosphatase and acute phase proteins were observed in the peritoneal fluid of G1 and G2 during the 14 days of evaluation (p ≤ 0.05), without causing clinically relevant peritonitis. Transcutaneous cecal trocarization promotes peritonitis, which is more intense with a cattle trocar than with a cecal needle. The cecal needle should be considered for cecal trocarization of horses with cecal tympany.
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spelling pubmed-96835932022-11-24 Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle Dória, Renata Gebara Sampaio Reginato, Gustavo Morandini Hayasaka, Yumi de Barcelos Fantinato Neto, Paulo Passarelli, Danielle Arantes, Julia de Assis PLoS One Research Article Percutaneous decompression of the cecum is a procedure that could be considered for horses with cecal gas distension. The aim of this study was to identify complications such as peritonitis and clinically relevant peritonitis (CRP) after transabdominal cecal trocarization in healthy horses using a cattle trocar and a cecal needle. Mixed breed horses were assigned to three groups (n = 6): horses that underwent trocarization with a cecal needle (G1) or a cattle trocar (G2), and a control group (CG) without cecal trocarization. The same horses were used in each group, respecting a three-month washout period between studies. A physical examination, serial blood, and peritoneal fluid sampling were performed, prior to cecal trocarization and 2, 6 and 12 hours after the first collection and 1, 2, 3, 7, and 14 days after the procedure. Acute-phase proteins in blood and peritoneal fluid were analyzed by polyacrylamide gel electrophoresis. Horses with a high cell count in the peritoneal fluid (i.e., 10,000 cells/μl) were considered to have peritonitis and CRP if they met at least two of the following clinical criteria: anorexia, lethargy, tachycardia, tachypnea, fever, ileus, abnormal oral mucous membrane color, abnormal white blood cells count, or high blood fibrinogen concentration (> 5 g/L). All horses recovered from cecal trocarization and abdominocentesis with no major complications. Cecal trocarization caused cytologic evidence of peritonitis in G1 and G2 during the 14 days of evaluation. CRP was not observed, although a decrease in cecal motility was observed in G1 and G2 during the experimental period and three horses, one from G1 and two from G2, showed a single moment of fever. None of the groups showed leukopenia or leukocytosis, although blood neutrophil count decreased at D7 and D14 in G1 and at D14 in G2 (p ≤ 0.05). After cecal trocarization, an increase in the total nucleated cells count, total proteins, globulins, alkaline phosphatase and acute phase proteins were observed in the peritoneal fluid of G1 and G2 during the 14 days of evaluation (p ≤ 0.05), without causing clinically relevant peritonitis. Transcutaneous cecal trocarization promotes peritonitis, which is more intense with a cattle trocar than with a cecal needle. The cecal needle should be considered for cecal trocarization of horses with cecal tympany. Public Library of Science 2022-11-23 /pmc/articles/PMC9683593/ /pubmed/36417417 http://dx.doi.org/10.1371/journal.pone.0277468 Text en © 2022 Dória et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dória, Renata Gebara Sampaio
Reginato, Gustavo Morandini
Hayasaka, Yumi de Barcelos
Fantinato Neto, Paulo
Passarelli, Danielle
Arantes, Julia de Assis
Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title_full Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title_fullStr Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title_full_unstemmed Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title_short Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
title_sort complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683593/
https://www.ncbi.nlm.nih.gov/pubmed/36417417
http://dx.doi.org/10.1371/journal.pone.0277468
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