A low-cost, open-source evolutionary bioreactor and its educational use

A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolut...

Descripción completa

Detalles Bibliográficos
Autores principales: Gopalakrishnan, Vishhvaan, Crozier, Dena, Card, Kyle J, Chick, Lacy D, Krishnan, Nikhil P, McClure, Erin, Pelesko, Julia, Williamson, Drew FK, Nichol, Daniel, Mandal, Soumyajit, Bonomo, Robert A, Scott, Jacob G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Evolutionary Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683782/
https://www.ncbi.nlm.nih.gov/pubmed/36317871
http://dx.doi.org/10.7554/eLife.83067
Descripción
Sumario:A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate Escherichia coli populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.

Ejemplares similares