A low-cost, open-source evolutionary bioreactor and its educational use

A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolut...

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Autores principales: Gopalakrishnan, Vishhvaan, Crozier, Dena, Card, Kyle J, Chick, Lacy D, Krishnan, Nikhil P, McClure, Erin, Pelesko, Julia, Williamson, Drew FK, Nichol, Daniel, Mandal, Soumyajit, Bonomo, Robert A, Scott, Jacob G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Evolutionary Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683782/
https://www.ncbi.nlm.nih.gov/pubmed/36317871
http://dx.doi.org/10.7554/eLife.83067
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author Gopalakrishnan, Vishhvaan
Crozier, Dena
Card, Kyle J
Chick, Lacy D
Krishnan, Nikhil P
McClure, Erin
Pelesko, Julia
Williamson, Drew FK
Nichol, Daniel
Mandal, Soumyajit
Bonomo, Robert A
Scott, Jacob G
author_facet Gopalakrishnan, Vishhvaan
Crozier, Dena
Card, Kyle J
Chick, Lacy D
Krishnan, Nikhil P
McClure, Erin
Pelesko, Julia
Williamson, Drew FK
Nichol, Daniel
Mandal, Soumyajit
Bonomo, Robert A
Scott, Jacob G
author_sort Gopalakrishnan, Vishhvaan
collection PubMed
description A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate Escherichia coli populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.
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spelling pubmed-96837822022-11-24 A low-cost, open-source evolutionary bioreactor and its educational use Gopalakrishnan, Vishhvaan Crozier, Dena Card, Kyle J Chick, Lacy D Krishnan, Nikhil P McClure, Erin Pelesko, Julia Williamson, Drew FK Nichol, Daniel Mandal, Soumyajit Bonomo, Robert A Scott, Jacob G eLife Evolutionary Biology A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate Escherichia coli populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance. eLife Sciences Publications, Ltd 2022-11-01 /pmc/articles/PMC9683782/ /pubmed/36317871 http://dx.doi.org/10.7554/eLife.83067 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Evolutionary Biology
Gopalakrishnan, Vishhvaan
Crozier, Dena
Card, Kyle J
Chick, Lacy D
Krishnan, Nikhil P
McClure, Erin
Pelesko, Julia
Williamson, Drew FK
Nichol, Daniel
Mandal, Soumyajit
Bonomo, Robert A
Scott, Jacob G
A low-cost, open-source evolutionary bioreactor and its educational use
title A low-cost, open-source evolutionary bioreactor and its educational use
title_full A low-cost, open-source evolutionary bioreactor and its educational use
title_fullStr A low-cost, open-source evolutionary bioreactor and its educational use
title_full_unstemmed A low-cost, open-source evolutionary bioreactor and its educational use
title_short A low-cost, open-source evolutionary bioreactor and its educational use
title_sort low-cost, open-source evolutionary bioreactor and its educational use
topic Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683782/
https://www.ncbi.nlm.nih.gov/pubmed/36317871
http://dx.doi.org/10.7554/eLife.83067
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