Cargando…
Topical Medicine Potency of Musa paradisiaca var. sapientum (L.) kuntze as Oral Gel for Wound Healing: An In Vitro , In Vivo Study
Objective Topical application of ambonese banana ( Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of t...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683894/ https://www.ncbi.nlm.nih.gov/pubmed/35181871 http://dx.doi.org/10.1055/s-0041-1740226 |
_version_ | 1784835154706956288 |
---|---|
author | Budi, Hendrik Setia Anitasari, Silvia Ulfa, Ninik Mas Juliastuti, Wisnu Setyari Aljunaid, Mohammed Ramadan, Doaa Elsayed Muzari, Koko Shen, Yung-Kang |
author_facet | Budi, Hendrik Setia Anitasari, Silvia Ulfa, Ninik Mas Juliastuti, Wisnu Setyari Aljunaid, Mohammed Ramadan, Doaa Elsayed Muzari, Koko Shen, Yung-Kang |
author_sort | Budi, Hendrik Setia |
collection | PubMed |
description | Objective Topical application of ambonese banana ( Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. Statistical analysis Data were analyzed using a one-way analysis of variance ( α = 0.05) with GraphPad Prism-8 statistical software. Results The study showed that the GEGPA formulation was stable against changes in consistency, color, smell, homogeneity, and pH value. There is a significant difference between groups with respect to viscosity ( p = 0.0001), adhesion ( p = 0.004), dispersion ( p = 0.000), and fibroblast cell numbers on days 3 and 5 ( p = 0.007 and p = 0.001). There is no interaction between the active ingredients and the gel base of all formulations. Formulation 3 had better properties in terms of viscosity, broad distribution, and drug release compared with other groups. Application of GEGPA to tooth extraction wounds showed a significant proliferation of fibroblast cells on days 3 and 5. Conclusions The formulation of M. paradisiaca var. sapientum (L.) kuntze extract with HPMC and propylene glycol obtained a gel preparation, GEGPA, that was organoleptically stable and met the topical gel standard for wounds in the oral cavity. |
format | Online Article Text |
id | pubmed-9683894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96838942022-11-24 Topical Medicine Potency of Musa paradisiaca var. sapientum (L.) kuntze as Oral Gel for Wound Healing: An In Vitro , In Vivo Study Budi, Hendrik Setia Anitasari, Silvia Ulfa, Ninik Mas Juliastuti, Wisnu Setyari Aljunaid, Mohammed Ramadan, Doaa Elsayed Muzari, Koko Shen, Yung-Kang Eur J Dent Objective Topical application of ambonese banana ( Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. Statistical analysis Data were analyzed using a one-way analysis of variance ( α = 0.05) with GraphPad Prism-8 statistical software. Results The study showed that the GEGPA formulation was stable against changes in consistency, color, smell, homogeneity, and pH value. There is a significant difference between groups with respect to viscosity ( p = 0.0001), adhesion ( p = 0.004), dispersion ( p = 0.000), and fibroblast cell numbers on days 3 and 5 ( p = 0.007 and p = 0.001). There is no interaction between the active ingredients and the gel base of all formulations. Formulation 3 had better properties in terms of viscosity, broad distribution, and drug release compared with other groups. Application of GEGPA to tooth extraction wounds showed a significant proliferation of fibroblast cells on days 3 and 5. Conclusions The formulation of M. paradisiaca var. sapientum (L.) kuntze extract with HPMC and propylene glycol obtained a gel preparation, GEGPA, that was organoleptically stable and met the topical gel standard for wounds in the oral cavity. Thieme Medical and Scientific Publishers Pvt. Ltd. 2022-02-18 /pmc/articles/PMC9683894/ /pubmed/35181871 http://dx.doi.org/10.1055/s-0041-1740226 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ) https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Budi, Hendrik Setia Anitasari, Silvia Ulfa, Ninik Mas Juliastuti, Wisnu Setyari Aljunaid, Mohammed Ramadan, Doaa Elsayed Muzari, Koko Shen, Yung-Kang Topical Medicine Potency of Musa paradisiaca var. sapientum (L.) kuntze as Oral Gel for Wound Healing: An In Vitro , In Vivo Study |
title |
Topical Medicine Potency of
Musa paradisiaca
var.
sapientum
(L.) kuntze as Oral Gel for Wound Healing: An
In Vitro
,
In Vivo
Study
|
title_full |
Topical Medicine Potency of
Musa paradisiaca
var.
sapientum
(L.) kuntze as Oral Gel for Wound Healing: An
In Vitro
,
In Vivo
Study
|
title_fullStr |
Topical Medicine Potency of
Musa paradisiaca
var.
sapientum
(L.) kuntze as Oral Gel for Wound Healing: An
In Vitro
,
In Vivo
Study
|
title_full_unstemmed |
Topical Medicine Potency of
Musa paradisiaca
var.
sapientum
(L.) kuntze as Oral Gel for Wound Healing: An
In Vitro
,
In Vivo
Study
|
title_short |
Topical Medicine Potency of
Musa paradisiaca
var.
sapientum
(L.) kuntze as Oral Gel for Wound Healing: An
In Vitro
,
In Vivo
Study
|
title_sort | topical medicine potency of
musa paradisiaca
var.
sapientum
(l.) kuntze as oral gel for wound healing: an
in vitro
,
in vivo
study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683894/ https://www.ncbi.nlm.nih.gov/pubmed/35181871 http://dx.doi.org/10.1055/s-0041-1740226 |
work_keys_str_mv | AT budihendriksetia topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT anitasarisilvia topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT ulfaninikmas topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT juliastutiwisnusetyari topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT aljunaidmohammed topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT ramadandoaaelsayed topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT muzarikoko topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy AT shenyungkang topicalmedicinepotencyofmusaparadisiacavarsapientumlkuntzeasoralgelforwoundhealinganinvitroinvivostudy |