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Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents

Blumea balsamifera (L.) DC., belonging to the Asteraceae family, also known as “ngai camphor,” is one of the traditional herbs used in Thailand for folk medicine and a component in local food and drinks. There was, however, no evidence indicating the presence of beneficial compounds at different lea...

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Autores principales: Jirakitticharoen, Sirinapha, Wisuitiprot, Wudtichai, Jitareerat, Pongphen, Wongs-Aree, Chalermchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683983/
https://www.ncbi.nlm.nih.gov/pubmed/36438181
http://dx.doi.org/10.1155/2022/7794227
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author Jirakitticharoen, Sirinapha
Wisuitiprot, Wudtichai
Jitareerat, Pongphen
Wongs-Aree, Chalermchai
author_facet Jirakitticharoen, Sirinapha
Wisuitiprot, Wudtichai
Jitareerat, Pongphen
Wongs-Aree, Chalermchai
author_sort Jirakitticharoen, Sirinapha
collection PubMed
description Blumea balsamifera (L.) DC., belonging to the Asteraceae family, also known as “ngai camphor,” is one of the traditional herbs used in Thailand for folk medicine and a component in local food and drinks. There was, however, no evidence indicating the presence of beneficial compounds at different leaf ages. Exploring various extraction solvents, we investigated the phenolics, flavonoids in particular quercetin content, antioxidant capacity, and antibacterial activity of immature and mature leaf extracts. The dried leaves were macerated in 50% ethanol, 95% ethanol, hexane, or decocted in water. Bioactive substances were analyzed by UV spectrophotometry and HPLC. Analysis of antioxidant capacity was done byDPPH, ABTS, FRAP, and NO scavenging assays. The antibacterial activity of immature leaf extract eluted with 50% ethanol was subsequentially evaluated in vitro. Extraction with 50% ethanol proved optimal, yielding 1.2–1.6-fold and 1.5-fold greater immature and mature leaf extracts than other solvents. More phenolics (1.2-fold), flavonoids (1.1-fold), quercetin content (4.8-fold), and antioxidant activity (1.3-fold) were found in the immature leaf extract. There was a significant positive correlation between antioxidant activity and bioactive compounds. The immature leaf extract eluted with 50% ethanol showed antibacterial activity against Staphylococcus aureus, with a minimum inhibitory concentration of 0.5 mg/mL. The immature leaves of B. balsamifera are a rich source of quercetin and phenolics, and 50% ethanol proved optimal for extracting bioactive components from these leaves.
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spelling pubmed-96839832022-11-24 Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents Jirakitticharoen, Sirinapha Wisuitiprot, Wudtichai Jitareerat, Pongphen Wongs-Aree, Chalermchai J Trop Med Research Article Blumea balsamifera (L.) DC., belonging to the Asteraceae family, also known as “ngai camphor,” is one of the traditional herbs used in Thailand for folk medicine and a component in local food and drinks. There was, however, no evidence indicating the presence of beneficial compounds at different leaf ages. Exploring various extraction solvents, we investigated the phenolics, flavonoids in particular quercetin content, antioxidant capacity, and antibacterial activity of immature and mature leaf extracts. The dried leaves were macerated in 50% ethanol, 95% ethanol, hexane, or decocted in water. Bioactive substances were analyzed by UV spectrophotometry and HPLC. Analysis of antioxidant capacity was done byDPPH, ABTS, FRAP, and NO scavenging assays. The antibacterial activity of immature leaf extract eluted with 50% ethanol was subsequentially evaluated in vitro. Extraction with 50% ethanol proved optimal, yielding 1.2–1.6-fold and 1.5-fold greater immature and mature leaf extracts than other solvents. More phenolics (1.2-fold), flavonoids (1.1-fold), quercetin content (4.8-fold), and antioxidant activity (1.3-fold) were found in the immature leaf extract. There was a significant positive correlation between antioxidant activity and bioactive compounds. The immature leaf extract eluted with 50% ethanol showed antibacterial activity against Staphylococcus aureus, with a minimum inhibitory concentration of 0.5 mg/mL. The immature leaves of B. balsamifera are a rich source of quercetin and phenolics, and 50% ethanol proved optimal for extracting bioactive components from these leaves. Hindawi 2022-11-16 /pmc/articles/PMC9683983/ /pubmed/36438181 http://dx.doi.org/10.1155/2022/7794227 Text en Copyright © 2022 Sirinapha Jirakitticharoen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jirakitticharoen, Sirinapha
Wisuitiprot, Wudtichai
Jitareerat, Pongphen
Wongs-Aree, Chalermchai
Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title_full Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title_fullStr Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title_full_unstemmed Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title_short Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
title_sort phenolics, antioxidant and antibacterial activities of immature and mature blumea balsamifera leaf extracts eluted with different solvents
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683983/
https://www.ncbi.nlm.nih.gov/pubmed/36438181
http://dx.doi.org/10.1155/2022/7794227
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