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Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii

Enterobacter cloacae is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated E. cloacae caused repeated infections in Macrobrachium rosenbergii, suggesting a high survival ability of the ba...

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Autores principales: Gao, Xiaojian, Qian, Qieqi, Zhu, Yujie, Chen, Zhen, Xu, Jingwen, Xu, Wenjing, Jiang, Qun, Wang, Jun, Zhang, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684176/
https://www.ncbi.nlm.nih.gov/pubmed/36439857
http://dx.doi.org/10.3389/fmicb.2022.1030955
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author Gao, Xiaojian
Qian, Qieqi
Zhu, Yujie
Chen, Zhen
Xu, Jingwen
Xu, Wenjing
Jiang, Qun
Wang, Jun
Zhang, Xiaojun
author_facet Gao, Xiaojian
Qian, Qieqi
Zhu, Yujie
Chen, Zhen
Xu, Jingwen
Xu, Wenjing
Jiang, Qun
Wang, Jun
Zhang, Xiaojun
author_sort Gao, Xiaojian
collection PubMed
description Enterobacter cloacae is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated E. cloacae caused repeated infections in Macrobrachium rosenbergii, suggesting a high survival ability of the bacteria, and rpoS gene has been known to regulate stress response and virulence of many bacteria. In this study, the E. cloacae-rpoS RNAi strain was constructed by RNAi technology, and the regulation role of rpoS in stress resistance and virulence of E. cloacae was explored by transcriptomic and phenotype analysis. The transcriptome analysis showed a total of 488 differentially expressed genes (DEGs) were identified between rpoS-RNAi and wild-type strains, including 30 up-regulated genes and 458 down-regulated genes, and these down-regulated DEGs were mainly related to environmental response, biofilm formation, bacterial type II secretory system, flagellin, fimbrillin, and chemotactic protein which associated with bacterial survival and virulence. The phenotype changes also showed the E. cloacae-rpoS RNAi strain exhibited significantly decreasing abilities of survival in environmental stresses (starvation, salinity, low pH, and oxidative stress), biofilm production, movement, adhesion to cells, pathogenicity, and colonization to M. rosenbergii. These results reveal that rpoS plays an important regulatory role in environmental stress adaptation and virulence of E. cloacae.
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spelling pubmed-96841762022-11-25 Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii Gao, Xiaojian Qian, Qieqi Zhu, Yujie Chen, Zhen Xu, Jingwen Xu, Wenjing Jiang, Qun Wang, Jun Zhang, Xiaojun Front Microbiol Microbiology Enterobacter cloacae is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated E. cloacae caused repeated infections in Macrobrachium rosenbergii, suggesting a high survival ability of the bacteria, and rpoS gene has been known to regulate stress response and virulence of many bacteria. In this study, the E. cloacae-rpoS RNAi strain was constructed by RNAi technology, and the regulation role of rpoS in stress resistance and virulence of E. cloacae was explored by transcriptomic and phenotype analysis. The transcriptome analysis showed a total of 488 differentially expressed genes (DEGs) were identified between rpoS-RNAi and wild-type strains, including 30 up-regulated genes and 458 down-regulated genes, and these down-regulated DEGs were mainly related to environmental response, biofilm formation, bacterial type II secretory system, flagellin, fimbrillin, and chemotactic protein which associated with bacterial survival and virulence. The phenotype changes also showed the E. cloacae-rpoS RNAi strain exhibited significantly decreasing abilities of survival in environmental stresses (starvation, salinity, low pH, and oxidative stress), biofilm production, movement, adhesion to cells, pathogenicity, and colonization to M. rosenbergii. These results reveal that rpoS plays an important regulatory role in environmental stress adaptation and virulence of E. cloacae. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9684176/ /pubmed/36439857 http://dx.doi.org/10.3389/fmicb.2022.1030955 Text en Copyright © 2022 Gao, Qian, Zhu, Chen, Xu, Xu, Jiang, Wang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gao, Xiaojian
Qian, Qieqi
Zhu, Yujie
Chen, Zhen
Xu, Jingwen
Xu, Wenjing
Jiang, Qun
Wang, Jun
Zhang, Xiaojun
Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title_full Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title_fullStr Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title_full_unstemmed Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title_short Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii
title_sort transcriptomic and phenotype analysis revealed the role of rpos in stress resistance and virulence of pathogenic enterobacter cloacae from macrobrachium rosenbergii
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684176/
https://www.ncbi.nlm.nih.gov/pubmed/36439857
http://dx.doi.org/10.3389/fmicb.2022.1030955
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