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Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its...

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Autores principales: Liu, Yawen, Wang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684326/
https://www.ncbi.nlm.nih.gov/pubmed/36439819
http://dx.doi.org/10.3389/fmicb.2022.1060506
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author Liu, Yawen
Wang, Li
author_facet Liu, Yawen
Wang, Li
author_sort Liu, Yawen
collection PubMed
description This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its possible antibiofilm mechanisms using transcriptomic analysis. The results indicated that the minimum antibacterial concentration of PCA against V. parahaemolyticus was 300 μg/mL. PCA (9.375 μg/mL) inhibited biofilm generation and adhesion of the mature biofilm. PCA (75 μg/mL) significantly reduced the metabolic viability of V. parahaemolyticus, reduced polysaccharide production, and inhibited cell surface flagella-mediated swimming and aggregation phenotypes. Meanwhile, transcriptome analysis showed that the key genes of V. parahaemolyticus expressed under PCA (75 μg/mL) inhibition were mainly related to biofilm formation (pfkA, galE, narL, and oppA), polysaccharide production and adhesion (IF, fbpA, and yxeM), and motility (cheY, flrC, and fliA). By regulating these key genes, PCA reduced biofilm formation, suppressed polysaccharide production and transport, and prevented the adhesion of V. parahaemolyticus, thereby reducing the virulence of V. parahaemolyticus. This study demonstrated that protocatechuic aldehyde can be used to control V. parahaemolyticus biofilm to ensure food safety.
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spelling pubmed-96843262022-11-25 Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus Liu, Yawen Wang, Li Front Microbiol Microbiology This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its possible antibiofilm mechanisms using transcriptomic analysis. The results indicated that the minimum antibacterial concentration of PCA against V. parahaemolyticus was 300 μg/mL. PCA (9.375 μg/mL) inhibited biofilm generation and adhesion of the mature biofilm. PCA (75 μg/mL) significantly reduced the metabolic viability of V. parahaemolyticus, reduced polysaccharide production, and inhibited cell surface flagella-mediated swimming and aggregation phenotypes. Meanwhile, transcriptome analysis showed that the key genes of V. parahaemolyticus expressed under PCA (75 μg/mL) inhibition were mainly related to biofilm formation (pfkA, galE, narL, and oppA), polysaccharide production and adhesion (IF, fbpA, and yxeM), and motility (cheY, flrC, and fliA). By regulating these key genes, PCA reduced biofilm formation, suppressed polysaccharide production and transport, and prevented the adhesion of V. parahaemolyticus, thereby reducing the virulence of V. parahaemolyticus. This study demonstrated that protocatechuic aldehyde can be used to control V. parahaemolyticus biofilm to ensure food safety. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9684326/ /pubmed/36439819 http://dx.doi.org/10.3389/fmicb.2022.1060506 Text en Copyright © 2022 Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yawen
Wang, Li
Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title_full Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title_fullStr Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title_full_unstemmed Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title_short Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
title_sort antibiofilm effect and mechanism of protocatechuic aldehyde against vibrio parahaemolyticus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684326/
https://www.ncbi.nlm.nih.gov/pubmed/36439819
http://dx.doi.org/10.3389/fmicb.2022.1060506
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