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Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus
This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684326/ https://www.ncbi.nlm.nih.gov/pubmed/36439819 http://dx.doi.org/10.3389/fmicb.2022.1060506 |
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author | Liu, Yawen Wang, Li |
author_facet | Liu, Yawen Wang, Li |
author_sort | Liu, Yawen |
collection | PubMed |
description | This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its possible antibiofilm mechanisms using transcriptomic analysis. The results indicated that the minimum antibacterial concentration of PCA against V. parahaemolyticus was 300 μg/mL. PCA (9.375 μg/mL) inhibited biofilm generation and adhesion of the mature biofilm. PCA (75 μg/mL) significantly reduced the metabolic viability of V. parahaemolyticus, reduced polysaccharide production, and inhibited cell surface flagella-mediated swimming and aggregation phenotypes. Meanwhile, transcriptome analysis showed that the key genes of V. parahaemolyticus expressed under PCA (75 μg/mL) inhibition were mainly related to biofilm formation (pfkA, galE, narL, and oppA), polysaccharide production and adhesion (IF, fbpA, and yxeM), and motility (cheY, flrC, and fliA). By regulating these key genes, PCA reduced biofilm formation, suppressed polysaccharide production and transport, and prevented the adhesion of V. parahaemolyticus, thereby reducing the virulence of V. parahaemolyticus. This study demonstrated that protocatechuic aldehyde can be used to control V. parahaemolyticus biofilm to ensure food safety. |
format | Online Article Text |
id | pubmed-9684326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96843262022-11-25 Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus Liu, Yawen Wang, Li Front Microbiol Microbiology This study investigated the effect of protocatechuic aldehyde (PCA) on Vibrio parahaemolyticus biofilm formation and its effects on gene expression. Crystal violet assay, metabolic activity assay, and fluorescence experiments were used to evaluate the antibiofilm activities of PCA and to reveal its possible antibiofilm mechanisms using transcriptomic analysis. The results indicated that the minimum antibacterial concentration of PCA against V. parahaemolyticus was 300 μg/mL. PCA (9.375 μg/mL) inhibited biofilm generation and adhesion of the mature biofilm. PCA (75 μg/mL) significantly reduced the metabolic viability of V. parahaemolyticus, reduced polysaccharide production, and inhibited cell surface flagella-mediated swimming and aggregation phenotypes. Meanwhile, transcriptome analysis showed that the key genes of V. parahaemolyticus expressed under PCA (75 μg/mL) inhibition were mainly related to biofilm formation (pfkA, galE, narL, and oppA), polysaccharide production and adhesion (IF, fbpA, and yxeM), and motility (cheY, flrC, and fliA). By regulating these key genes, PCA reduced biofilm formation, suppressed polysaccharide production and transport, and prevented the adhesion of V. parahaemolyticus, thereby reducing the virulence of V. parahaemolyticus. This study demonstrated that protocatechuic aldehyde can be used to control V. parahaemolyticus biofilm to ensure food safety. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9684326/ /pubmed/36439819 http://dx.doi.org/10.3389/fmicb.2022.1060506 Text en Copyright © 2022 Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Yawen Wang, Li Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title | Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title_full | Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title_fullStr | Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title_full_unstemmed | Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title_short | Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus |
title_sort | antibiofilm effect and mechanism of protocatechuic aldehyde against vibrio parahaemolyticus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684326/ https://www.ncbi.nlm.nih.gov/pubmed/36439819 http://dx.doi.org/10.3389/fmicb.2022.1060506 |
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