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Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression

Glucagon is a major regulator of metabolism and drugs targeting the glucagon receptor (GCGR) are being developed. Insight into tissue and cell-specific expression of the GCGR is important to understand the biology of glucagon and to differentiate between direct and indirect actions of glucagon. Howe...

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Detalles Bibliográficos
Autores principales: Bomholt, Anna Billeschou, Johansen, Christian Dall, Christensen, Jens Bager, Kjeldsen, Sasha Alexandra Sampson, Galsgaard, Katrine Douglas, Winther-Sørensen, Marie, Serizawa, Reza, Hornum, Mads, Porrini, Esteban, Pedersen, Jens, Ørskov, Cathrine, Gluud, Lise Lotte, Sørensen, Charlotte Mehlin, Holst, Jens Juul, Albrechtsen, Reidar, Wewer Albrechtsen, Nicolai Jacob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684523/
https://www.ncbi.nlm.nih.gov/pubmed/36418521
http://dx.doi.org/10.1038/s42003-022-04242-7
Descripción
Sumario:Glucagon is a major regulator of metabolism and drugs targeting the glucagon receptor (GCGR) are being developed. Insight into tissue and cell-specific expression of the GCGR is important to understand the biology of glucagon and to differentiate between direct and indirect actions of glucagon. However, it has been challenging to localize the GCGR in tissue due to low expression levels and lack of specific methods. Immunohistochemistry has frequently been used for GCGR localization, but antibodies targeting G-protein-coupled-receptors may be inaccurate. We evaluated all currently commercially available GCGR antibodies. The antibody, ab75240 (Antibody no. 11) was found to perform best among the twelve antibodies tested and using this antibody we found expression of the GCGR in the kidney, liver, preadipocytes, pancreas, and heart. Three antibody-independent approaches all confirmed the presence of the GCGR within the pancreas, liver and the kidneys. GCGR expression should be evaluated by both antibody and antibody-independent approaches.