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1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines

With the development of laser technology in the 1960s, a technique was developed to inject intradermal vaccines immediately after irradiating the skin with laser light to elicit an adjuvant effect, referred to as “laser adjuvant.” We have been investigating the mechanism of laser adjuvant in influen...

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Autores principales: Maki, Yohei, Kushibiki, Toshihiro, Sano, Tomoya, Ogawa, Takunori, Komai, Eri, Takahashi, Shusaku, Kitagami, Etsuko, Serizawa, Yusuke, Nagaoka, Ryosuke, Yokomizo, Shinya, Ono, Takeshi, Ishihara, Miya, Miyahira, Yasushi, Kashiwagi, Satoshi, Kawana, Akihiko, Kimizuka, Yoshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684730/
https://www.ncbi.nlm.nih.gov/pubmed/36439134
http://dx.doi.org/10.3389/fimmu.2022.1028733
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author Maki, Yohei
Kushibiki, Toshihiro
Sano, Tomoya
Ogawa, Takunori
Komai, Eri
Takahashi, Shusaku
Kitagami, Etsuko
Serizawa, Yusuke
Nagaoka, Ryosuke
Yokomizo, Shinya
Ono, Takeshi
Ishihara, Miya
Miyahira, Yasushi
Kashiwagi, Satoshi
Kawana, Akihiko
Kimizuka, Yoshifumi
author_facet Maki, Yohei
Kushibiki, Toshihiro
Sano, Tomoya
Ogawa, Takunori
Komai, Eri
Takahashi, Shusaku
Kitagami, Etsuko
Serizawa, Yusuke
Nagaoka, Ryosuke
Yokomizo, Shinya
Ono, Takeshi
Ishihara, Miya
Miyahira, Yasushi
Kashiwagi, Satoshi
Kawana, Akihiko
Kimizuka, Yoshifumi
author_sort Maki, Yohei
collection PubMed
description With the development of laser technology in the 1960s, a technique was developed to inject intradermal vaccines immediately after irradiating the skin with laser light to elicit an adjuvant effect, referred to as “laser adjuvant.” We have been investigating the mechanism of laser adjuvant in influenza mouse models using noninvasive continuous-wave (CW) near-infrared (NIR) light mainly at a wavelength of 1064 nm, and have shown that the production of reactive-oxygen-species (ROS) in the skin and mast cells in the skin tissue plays an important role in the laser adjuvant effect. The new wavelength of 1270 nm NIR light is characterized by its ability to elicit the same vaccine adjuvant effect as other wavelengths at a lower energy, and may be suitable for clinical applications. In this study, we investigated the physiological activity of CW1270 nm NIR light in mast cells, its biological activity on mouse skin, and the durability of the vaccine adjuvant effect in influenza vaccine mouse models. We show that irradiation of mast cells with 1270 nm NIR light produced ROS and ATP, and irradiation of isolated mitochondria also produced ATP. In mouse skin, the relative expression levels of chemokine mRNAs, such as Ccl2 and Ccl20, were increased by irradiation with 1270 and 1064 nm NIR light at minimum safe irradiance. However, the relative expression of Nfkb1 was increased at 1064 nm, but not at 1270 nm. Serum anti-influenza IgG antibody titers increased early after immunization with 1064 nm, whereas with 1270 nm, there was not only an early response of antibody production but also persistence of antibody titers over the medium- to long-term. Thus, to our knowledge, we show for the first time that 1270 nm NIR light induces ROS and ATP production in mitochondria as photoreceptors, initiating a cascade of laser adjuvant effects for intradermal vaccines. Additionally, we demonstrate that there are wavelength-specific variations in the mechanisms and effects of laser adjuvants. In conclusion, CW1270 nm NIR light is expected to be clinically applicable as a novel laser adjuvant that is equivalent or superior to 1064 nm NIR light, because it can be operated at low energy and has a wavelength-specific adjuvant effect with medium- to long-lasting antibody titer.
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spelling pubmed-96847302022-11-25 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines Maki, Yohei Kushibiki, Toshihiro Sano, Tomoya Ogawa, Takunori Komai, Eri Takahashi, Shusaku Kitagami, Etsuko Serizawa, Yusuke Nagaoka, Ryosuke Yokomizo, Shinya Ono, Takeshi Ishihara, Miya Miyahira, Yasushi Kashiwagi, Satoshi Kawana, Akihiko Kimizuka, Yoshifumi Front Immunol Immunology With the development of laser technology in the 1960s, a technique was developed to inject intradermal vaccines immediately after irradiating the skin with laser light to elicit an adjuvant effect, referred to as “laser adjuvant.” We have been investigating the mechanism of laser adjuvant in influenza mouse models using noninvasive continuous-wave (CW) near-infrared (NIR) light mainly at a wavelength of 1064 nm, and have shown that the production of reactive-oxygen-species (ROS) in the skin and mast cells in the skin tissue plays an important role in the laser adjuvant effect. The new wavelength of 1270 nm NIR light is characterized by its ability to elicit the same vaccine adjuvant effect as other wavelengths at a lower energy, and may be suitable for clinical applications. In this study, we investigated the physiological activity of CW1270 nm NIR light in mast cells, its biological activity on mouse skin, and the durability of the vaccine adjuvant effect in influenza vaccine mouse models. We show that irradiation of mast cells with 1270 nm NIR light produced ROS and ATP, and irradiation of isolated mitochondria also produced ATP. In mouse skin, the relative expression levels of chemokine mRNAs, such as Ccl2 and Ccl20, were increased by irradiation with 1270 and 1064 nm NIR light at minimum safe irradiance. However, the relative expression of Nfkb1 was increased at 1064 nm, but not at 1270 nm. Serum anti-influenza IgG antibody titers increased early after immunization with 1064 nm, whereas with 1270 nm, there was not only an early response of antibody production but also persistence of antibody titers over the medium- to long-term. Thus, to our knowledge, we show for the first time that 1270 nm NIR light induces ROS and ATP production in mitochondria as photoreceptors, initiating a cascade of laser adjuvant effects for intradermal vaccines. Additionally, we demonstrate that there are wavelength-specific variations in the mechanisms and effects of laser adjuvants. In conclusion, CW1270 nm NIR light is expected to be clinically applicable as a novel laser adjuvant that is equivalent or superior to 1064 nm NIR light, because it can be operated at low energy and has a wavelength-specific adjuvant effect with medium- to long-lasting antibody titer. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9684730/ /pubmed/36439134 http://dx.doi.org/10.3389/fimmu.2022.1028733 Text en Copyright © 2022 Maki, Kushibiki, Sano, Ogawa, Komai, Takahashi, Kitagami, Serizawa, Nagaoka, Yokomizo, Ono, Ishihara, Miyahira, Kashiwagi, Kawana and Kimizuka https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Maki, Yohei
Kushibiki, Toshihiro
Sano, Tomoya
Ogawa, Takunori
Komai, Eri
Takahashi, Shusaku
Kitagami, Etsuko
Serizawa, Yusuke
Nagaoka, Ryosuke
Yokomizo, Shinya
Ono, Takeshi
Ishihara, Miya
Miyahira, Yasushi
Kashiwagi, Satoshi
Kawana, Akihiko
Kimizuka, Yoshifumi
1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title_full 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title_fullStr 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title_full_unstemmed 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title_short 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
title_sort 1270 nm near-infrared light as a novel vaccine adjuvant acts on mitochondrial photoreception in intradermal vaccines
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684730/
https://www.ncbi.nlm.nih.gov/pubmed/36439134
http://dx.doi.org/10.3389/fimmu.2022.1028733
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