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Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity

BACKGROUND: Walking is the preferred therapy for peripheral arterial disease in early stage. An effect of walking exercise is the increase of blood flow and fluid shear stress, leading, triggered by arteriogenesis, to the formation of collateral blood vessels. Circulating micro-RNA may act as an imp...

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Autores principales: Sieland, Johanna, Niederer, Daniel, Engeroff, Tobias, Vogt, Lutz, Troidl, Christian, Schmitz-Rixen, Thomas, Banzer, Winfried, Troidl, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684818/
https://www.ncbi.nlm.nih.gov/pubmed/36418750
http://dx.doi.org/10.1007/s00421-022-05091-2
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author Sieland, Johanna
Niederer, Daniel
Engeroff, Tobias
Vogt, Lutz
Troidl, Christian
Schmitz-Rixen, Thomas
Banzer, Winfried
Troidl, Kerstin
author_facet Sieland, Johanna
Niederer, Daniel
Engeroff, Tobias
Vogt, Lutz
Troidl, Christian
Schmitz-Rixen, Thomas
Banzer, Winfried
Troidl, Kerstin
author_sort Sieland, Johanna
collection PubMed
description BACKGROUND: Walking is the preferred therapy for peripheral arterial disease in early stage. An effect of walking exercise is the increase of blood flow and fluid shear stress, leading, triggered by arteriogenesis, to the formation of collateral blood vessels. Circulating micro-RNA may act as an important information transmitter in this process. We investigated the acute effects of a single bout of 1) aerobic walking with moderate intensity; and 2) anaerobic walking with vigorous intensity on miRNA parameters related to vascular collateral formation. METHODS: Ten (10) patients with peripheral arterial disease with claudication (age 72 ± 7 years) participated in this two-armed, randomized-balanced cross-over study. The intervention arms were single bouts of supervised walking training at (1) vigorous intensity on a treadmill up to volitional exhaustion and (2) moderate intensity with individual selected speed for a duration of 20 min. One week of washout was maintained between the arms. During each intervention, heart rate was continuously monitored. Acute effects on circulating miRNAs and lactate concentration were determined using pre- and post-intervention measurement comparisons. RESULTS: Vigorous-intensity walking resulted in a higher heart rate (125 ± 21 bpm) than the moderate-intensity intervention (88 ± 9 bpm) (p < 0.05). Lactate concentration was increased after vigorous-intensity walking (p = 0.005; 3.3 ± 1.2 mmol/l), but not after moderate exercising (p > 0.05; 1.7 ± 0.6 mmol/l). The circulating levels of miR-142-5p and miR-424-5p were up-regulated after moderate-intensity (p < 0.05), but not after vigorous-intensity training (p > 0.05). CONCLUSION: Moderate-intensity walking seems to be more feasible than vigorous exercises to induce changes of blood flow and endurance training-related miRNAs in patients with peripheral arterial disease. Our data thus indicates that effect mechanisms might follow an optimal rather than a maximal dose response relation. Steady state walking without the necessity to reach exhaustion seems to be better suited as stimulus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00421-022-05091-2.
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spelling pubmed-96848182022-11-28 Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity Sieland, Johanna Niederer, Daniel Engeroff, Tobias Vogt, Lutz Troidl, Christian Schmitz-Rixen, Thomas Banzer, Winfried Troidl, Kerstin Eur J Appl Physiol Original Article BACKGROUND: Walking is the preferred therapy for peripheral arterial disease in early stage. An effect of walking exercise is the increase of blood flow and fluid shear stress, leading, triggered by arteriogenesis, to the formation of collateral blood vessels. Circulating micro-RNA may act as an important information transmitter in this process. We investigated the acute effects of a single bout of 1) aerobic walking with moderate intensity; and 2) anaerobic walking with vigorous intensity on miRNA parameters related to vascular collateral formation. METHODS: Ten (10) patients with peripheral arterial disease with claudication (age 72 ± 7 years) participated in this two-armed, randomized-balanced cross-over study. The intervention arms were single bouts of supervised walking training at (1) vigorous intensity on a treadmill up to volitional exhaustion and (2) moderate intensity with individual selected speed for a duration of 20 min. One week of washout was maintained between the arms. During each intervention, heart rate was continuously monitored. Acute effects on circulating miRNAs and lactate concentration were determined using pre- and post-intervention measurement comparisons. RESULTS: Vigorous-intensity walking resulted in a higher heart rate (125 ± 21 bpm) than the moderate-intensity intervention (88 ± 9 bpm) (p < 0.05). Lactate concentration was increased after vigorous-intensity walking (p = 0.005; 3.3 ± 1.2 mmol/l), but not after moderate exercising (p > 0.05; 1.7 ± 0.6 mmol/l). The circulating levels of miR-142-5p and miR-424-5p were up-regulated after moderate-intensity (p < 0.05), but not after vigorous-intensity training (p > 0.05). CONCLUSION: Moderate-intensity walking seems to be more feasible than vigorous exercises to induce changes of blood flow and endurance training-related miRNAs in patients with peripheral arterial disease. Our data thus indicates that effect mechanisms might follow an optimal rather than a maximal dose response relation. Steady state walking without the necessity to reach exhaustion seems to be better suited as stimulus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00421-022-05091-2. Springer Berlin Heidelberg 2022-11-23 2023 /pmc/articles/PMC9684818/ /pubmed/36418750 http://dx.doi.org/10.1007/s00421-022-05091-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Sieland, Johanna
Niederer, Daniel
Engeroff, Tobias
Vogt, Lutz
Troidl, Christian
Schmitz-Rixen, Thomas
Banzer, Winfried
Troidl, Kerstin
Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title_full Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title_fullStr Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title_full_unstemmed Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title_short Changes in miRNA expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
title_sort changes in mirna expression in patients with peripheral arterial vascular disease during moderate- and vigorous-intensity physical activity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9684818/
https://www.ncbi.nlm.nih.gov/pubmed/36418750
http://dx.doi.org/10.1007/s00421-022-05091-2
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