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Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2
The COVID-19 pandemic involving SARS-CoV-2 has raised interest in using antimicrobial lipid formulations to inhibit viral entry into their host cells or to inactivate them. Lipids are a part of the innate defense mechanism against pathogens. Here, we evaluated the use of nano-monocaprin (NMC) in inh...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685086/ https://www.ncbi.nlm.nih.gov/pubmed/36422769 http://dx.doi.org/10.1007/s10123-022-00300-6 |
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author | Sorinolu, Adeola Julian Mamun, M. Mustafa Vadarevu, Hemapriyadarshini Vivero-Escoto, Juan L. Vejerano, Eric P. Munir, Mariya |
author_facet | Sorinolu, Adeola Julian Mamun, M. Mustafa Vadarevu, Hemapriyadarshini Vivero-Escoto, Juan L. Vejerano, Eric P. Munir, Mariya |
author_sort | Sorinolu, Adeola Julian |
collection | PubMed |
description | The COVID-19 pandemic involving SARS-CoV-2 has raised interest in using antimicrobial lipid formulations to inhibit viral entry into their host cells or to inactivate them. Lipids are a part of the innate defense mechanism against pathogens. Here, we evaluated the use of nano-monocaprin (NMC) in inhibiting enveloped (phi6) and unenveloped (MS2) bacteriophages. NMC was prepared using the sonochemistry technique. Size and morphology analysis revealed the formation of ~ 8.4 ± 0.2-nm NMC as measured by dynamic light scattering. We compared the antiviral activity of NMC with molecular monocaprin (MMC) at 0.5 mM and 2 mM concentrations against phi6, which we used as a surrogate for SARS-CoV-2. The synthesized NMC exhibited 50% higher antiviral activity against phi6 than MMC at pH 7 using plaque assay. NMC inactivated phi6 stronger at pH 4 than at pH 7. To determine if NMC is toxic to mammalian cells, we used MTS assay to assess its IC(50) for HPDE and HeLa cell lines, which were ~ 203 and 221 µM, respectively. NMC may be used for prophylactic application either as a drop or spray since many viruses enter the human body through the mucosal lining of the nose, eyes, and lungs. |
format | Online Article Text |
id | pubmed-9685086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-96850862022-11-28 Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 Sorinolu, Adeola Julian Mamun, M. Mustafa Vadarevu, Hemapriyadarshini Vivero-Escoto, Juan L. Vejerano, Eric P. Munir, Mariya Int Microbiol Research The COVID-19 pandemic involving SARS-CoV-2 has raised interest in using antimicrobial lipid formulations to inhibit viral entry into their host cells or to inactivate them. Lipids are a part of the innate defense mechanism against pathogens. Here, we evaluated the use of nano-monocaprin (NMC) in inhibiting enveloped (phi6) and unenveloped (MS2) bacteriophages. NMC was prepared using the sonochemistry technique. Size and morphology analysis revealed the formation of ~ 8.4 ± 0.2-nm NMC as measured by dynamic light scattering. We compared the antiviral activity of NMC with molecular monocaprin (MMC) at 0.5 mM and 2 mM concentrations against phi6, which we used as a surrogate for SARS-CoV-2. The synthesized NMC exhibited 50% higher antiviral activity against phi6 than MMC at pH 7 using plaque assay. NMC inactivated phi6 stronger at pH 4 than at pH 7. To determine if NMC is toxic to mammalian cells, we used MTS assay to assess its IC(50) for HPDE and HeLa cell lines, which were ~ 203 and 221 µM, respectively. NMC may be used for prophylactic application either as a drop or spray since many viruses enter the human body through the mucosal lining of the nose, eyes, and lungs. Springer International Publishing 2022-11-24 2023 /pmc/articles/PMC9685086/ /pubmed/36422769 http://dx.doi.org/10.1007/s10123-022-00300-6 Text en © The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Sorinolu, Adeola Julian Mamun, M. Mustafa Vadarevu, Hemapriyadarshini Vivero-Escoto, Juan L. Vejerano, Eric P. Munir, Mariya Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title | Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title_full | Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title_fullStr | Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title_full_unstemmed | Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title_short | Antiviral activity of nano-monocaprin against Phi6 as a surrogate for SARS-CoV-2 |
title_sort | antiviral activity of nano-monocaprin against phi6 as a surrogate for sars-cov-2 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685086/ https://www.ncbi.nlm.nih.gov/pubmed/36422769 http://dx.doi.org/10.1007/s10123-022-00300-6 |
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