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Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress

Chondrocytes are indispensable for the function of cartilage because they provide the extracellular matrix. Therefore, gaining insight into the chondrocytes may be helpful in understanding cartilage function and pinpointing potential therapeutical targets for diseases. The talus is a part of the ank...

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Autores principales: Wang, Tianrui, Wang, Junjie, Sun, Zewen, Zhang, Lu, Yu, Chenghao, Zhao, Haibo, Yan, Mingyue, Sun, Shenjie, Ye, Zhenhao, Zhang, Yingze, Yu, Tengbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685414/
https://www.ncbi.nlm.nih.gov/pubmed/36438550
http://dx.doi.org/10.3389/fcell.2022.1047119
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author Wang, Tianrui
Wang, Junjie
Sun, Zewen
Zhang, Lu
Yu, Chenghao
Zhao, Haibo
Yan, Mingyue
Sun, Shenjie
Ye, Zhenhao
Zhang, Yingze
Yu, Tengbo
author_facet Wang, Tianrui
Wang, Junjie
Sun, Zewen
Zhang, Lu
Yu, Chenghao
Zhao, Haibo
Yan, Mingyue
Sun, Shenjie
Ye, Zhenhao
Zhang, Yingze
Yu, Tengbo
author_sort Wang, Tianrui
collection PubMed
description Chondrocytes are indispensable for the function of cartilage because they provide the extracellular matrix. Therefore, gaining insight into the chondrocytes may be helpful in understanding cartilage function and pinpointing potential therapeutical targets for diseases. The talus is a part of the ankle joint, which serves as the major large joint that bears body weight. Compared with the distal tibial and fibula, the talus bears much more mechanical loading, which is a risk factor for osteoarthritis (OA). However, in most individuals, OA seems to be absent in the ankle, and the cartilage of the talus seems to function normally. This study applied single-cell RNA sequencing to demonstrate atlas for chondrocyte subsets in healthy talus cartilage obtained from five volunteers, and chondrocyte subsets were annotated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses for each cell type, cell–cell interactions, and single-cell regulatory network inference and clustering for each cell type were conducted, and hub genes for each cell type were identified. Immunohistochemical staining was used to confirm the presence and distribution of each cell type. Two new chondrocyte subsets were annotated as MirCs and SpCs. The identified and speculated novel microenvironment may pose different directions in chondrocyte composition, development, and metabolism in the talus.
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spelling pubmed-96854142022-11-25 Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress Wang, Tianrui Wang, Junjie Sun, Zewen Zhang, Lu Yu, Chenghao Zhao, Haibo Yan, Mingyue Sun, Shenjie Ye, Zhenhao Zhang, Yingze Yu, Tengbo Front Cell Dev Biol Cell and Developmental Biology Chondrocytes are indispensable for the function of cartilage because they provide the extracellular matrix. Therefore, gaining insight into the chondrocytes may be helpful in understanding cartilage function and pinpointing potential therapeutical targets for diseases. The talus is a part of the ankle joint, which serves as the major large joint that bears body weight. Compared with the distal tibial and fibula, the talus bears much more mechanical loading, which is a risk factor for osteoarthritis (OA). However, in most individuals, OA seems to be absent in the ankle, and the cartilage of the talus seems to function normally. This study applied single-cell RNA sequencing to demonstrate atlas for chondrocyte subsets in healthy talus cartilage obtained from five volunteers, and chondrocyte subsets were annotated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses for each cell type, cell–cell interactions, and single-cell regulatory network inference and clustering for each cell type were conducted, and hub genes for each cell type were identified. Immunohistochemical staining was used to confirm the presence and distribution of each cell type. Two new chondrocyte subsets were annotated as MirCs and SpCs. The identified and speculated novel microenvironment may pose different directions in chondrocyte composition, development, and metabolism in the talus. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9685414/ /pubmed/36438550 http://dx.doi.org/10.3389/fcell.2022.1047119 Text en Copyright © 2022 Wang, Wang, Sun, Zhang, Yu, Zhao, Yan, Sun, Ye, Zhang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wang, Tianrui
Wang, Junjie
Sun, Zewen
Zhang, Lu
Yu, Chenghao
Zhao, Haibo
Yan, Mingyue
Sun, Shenjie
Ye, Zhenhao
Zhang, Yingze
Yu, Tengbo
Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title_full Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title_fullStr Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title_full_unstemmed Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title_short Single-cell RNA sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
title_sort single-cell rna sequence presents atlas analysis for chondrocytes in the talus and reveals the potential mechanism in coping with mechanical stress
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685414/
https://www.ncbi.nlm.nih.gov/pubmed/36438550
http://dx.doi.org/10.3389/fcell.2022.1047119
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