Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector

BACKGROUND: Due to its high expression capability, recombination of Escherichia coli and pET vector has become the bioengineering preferred expression system. Because β-lactamases mediate bacterial antimicrobial resistance, these enzymes have a substantial clinical impact. Using the E. coli expressi...

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Detalles Bibliográficos
Autores principales: Li, Lele, Li, Hui, Tian, Qingwu, Ge, Baosheng, Xu, Xiaotong, Chi, Yuanyuan, Zhao, Huaizhi, Liu, Yanfei, Jia, Nan, Zhou, Tingting, Zhu, Yuanqi, Zhou, Yusun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686023/
https://www.ncbi.nlm.nih.gov/pubmed/36419169
http://dx.doi.org/10.1186/s12934-022-01972-5

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