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Functions of retinal astrocytes and Müller cells in mammalian myopia

BACKGROUND: Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form dep...

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Autores principales: Zhang, Xuhong, Yu, Xin, Wen, Yingying, Jin, Le, Zhang, Liyue, Zhu, Hong, Zhang, Dongyan, Xie, Chen, Guo, Dongyu, Tong, Jianping, Shen, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686084/
https://www.ncbi.nlm.nih.gov/pubmed/36418970
http://dx.doi.org/10.1186/s12886-022-02643-0
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author Zhang, Xuhong
Yu, Xin
Wen, Yingying
Jin, Le
Zhang, Liyue
Zhu, Hong
Zhang, Dongyan
Xie, Chen
Guo, Dongyu
Tong, Jianping
Shen, Ye
author_facet Zhang, Xuhong
Yu, Xin
Wen, Yingying
Jin, Le
Zhang, Liyue
Zhu, Hong
Zhang, Dongyan
Xie, Chen
Guo, Dongyu
Tong, Jianping
Shen, Ye
author_sort Zhang, Xuhong
collection PubMed
description BACKGROUND: Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form deprived myopia. METHODS: We adapted the mice form-deprivation myopia model by covering the right eye and left the left eye open for control, measured the ocular structure with anterior segment optical coherence tomography, evaluated changes in the morphology and distribution of retinal glial cells by fluorescence staining and western blotting; we also searched the online GEO databases to obtain relative gene lists and confirmed them in the form-deprivation myopia mouse retina at mRNA and protein level. RESULTS: Compared with the open eye, the ocular axial length (3.54 ± 0.006 mm v.s. 3.48 ± 0.004 mm, p = 0.027) and vitreous chamber depth (3.07 ± 0.005 mm v.s. 2.98 ± 0.006 mm, p = 0.007) in the covered eye became longer. Both glial fibrillary acidic protein and excitatory amino acid transporters 4 elevated. There were 12 common pathways in human myopia and anoxic astrocytes. The key proteins were also highly relevant to atropine target proteins. In mice, two common pathways were found in myopia and anoxic Müller cells. Seven main genes and four key proteins were significantly changed in the mice form-deprivation myopia retinas. CONCLUSION: Retinal astrocytes and Müller cells were activated in myopia. They may response to stimuli and secretory acting factors, and might be a valid target for atropine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12886-022-02643-0.
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spelling pubmed-96860842022-11-25 Functions of retinal astrocytes and Müller cells in mammalian myopia Zhang, Xuhong Yu, Xin Wen, Yingying Jin, Le Zhang, Liyue Zhu, Hong Zhang, Dongyan Xie, Chen Guo, Dongyu Tong, Jianping Shen, Ye BMC Ophthalmol Research BACKGROUND: Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form deprived myopia. METHODS: We adapted the mice form-deprivation myopia model by covering the right eye and left the left eye open for control, measured the ocular structure with anterior segment optical coherence tomography, evaluated changes in the morphology and distribution of retinal glial cells by fluorescence staining and western blotting; we also searched the online GEO databases to obtain relative gene lists and confirmed them in the form-deprivation myopia mouse retina at mRNA and protein level. RESULTS: Compared with the open eye, the ocular axial length (3.54 ± 0.006 mm v.s. 3.48 ± 0.004 mm, p = 0.027) and vitreous chamber depth (3.07 ± 0.005 mm v.s. 2.98 ± 0.006 mm, p = 0.007) in the covered eye became longer. Both glial fibrillary acidic protein and excitatory amino acid transporters 4 elevated. There were 12 common pathways in human myopia and anoxic astrocytes. The key proteins were also highly relevant to atropine target proteins. In mice, two common pathways were found in myopia and anoxic Müller cells. Seven main genes and four key proteins were significantly changed in the mice form-deprivation myopia retinas. CONCLUSION: Retinal astrocytes and Müller cells were activated in myopia. They may response to stimuli and secretory acting factors, and might be a valid target for atropine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12886-022-02643-0. BioMed Central 2022-11-24 /pmc/articles/PMC9686084/ /pubmed/36418970 http://dx.doi.org/10.1186/s12886-022-02643-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Xuhong
Yu, Xin
Wen, Yingying
Jin, Le
Zhang, Liyue
Zhu, Hong
Zhang, Dongyan
Xie, Chen
Guo, Dongyu
Tong, Jianping
Shen, Ye
Functions of retinal astrocytes and Müller cells in mammalian myopia
title Functions of retinal astrocytes and Müller cells in mammalian myopia
title_full Functions of retinal astrocytes and Müller cells in mammalian myopia
title_fullStr Functions of retinal astrocytes and Müller cells in mammalian myopia
title_full_unstemmed Functions of retinal astrocytes and Müller cells in mammalian myopia
title_short Functions of retinal astrocytes and Müller cells in mammalian myopia
title_sort functions of retinal astrocytes and müller cells in mammalian myopia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686084/
https://www.ncbi.nlm.nih.gov/pubmed/36418970
http://dx.doi.org/10.1186/s12886-022-02643-0
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