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Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor

Bioreactors are one of the most important, basic pieces of equipment in the biopharmaceutical industry. Understanding the effects of mechanical damage and other factors on the physiological state of cells during cell matrix culture is the basis for continuously achieving greater efficiency and highe...

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Autores principales: Yu, Shouzhi, Guo, Miaomiao, Zhang, Yadan, Bo, Cunpei, Liang, Hongyang, Wang, Hui, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686226/
https://www.ncbi.nlm.nih.gov/pubmed/36424406
http://dx.doi.org/10.1038/s41598-022-24687-4
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author Yu, Shouzhi
Guo, Miaomiao
Zhang, Yadan
Bo, Cunpei
Liang, Hongyang
Wang, Hui
Yang, Xiaoming
author_facet Yu, Shouzhi
Guo, Miaomiao
Zhang, Yadan
Bo, Cunpei
Liang, Hongyang
Wang, Hui
Yang, Xiaoming
author_sort Yu, Shouzhi
collection PubMed
description Bioreactors are one of the most important, basic pieces of equipment in the biopharmaceutical industry. Understanding the effects of mechanical damage and other factors on the physiological state of cells during cell matrix culture is the basis for continuously achieving greater efficiency and higher product quality. In this study, Vero cells were used as a model and apoptosis, senescence, transcriptomics, proteomics, and metabolomics were carried out for analysis at the cellular and molecular levels. The results showed that compared with cells cultured in the simulated natural state, the cells cultured in the basket bioreactor displayed no obvious senescence. Additionally, the proportion of early apoptotic cells increased, but the proportions of damaged, late apoptotic and dead cells did not change significantly. The transcription levels of aminoacyl-tRNA synthetase and cyclin D1 and the expression levels of DNA replication licensing factor, methenyltetrahydrofolate cyclohydrolase, arachidonic acid and other metabolites of cells cultured in the basket bioreactor were significantly increased. These results suggest that DNA replication, protein translation and the metabolic activities in cells cultured in basket bioreactors are more active, which is more conducive to cell amplification and target product production. In this study, the growth and physiological state of cells in a basket bioreactor were characterized at the molecular level for the first time. Additionally, a tool to evaluate the physiological state of cells in a bioreactor was established, which can be used to guide the development and optimization of cell matrix culture conditions in industrial production and improve the production efficiency of the target products.
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spelling pubmed-96862262022-11-26 Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor Yu, Shouzhi Guo, Miaomiao Zhang, Yadan Bo, Cunpei Liang, Hongyang Wang, Hui Yang, Xiaoming Sci Rep Article Bioreactors are one of the most important, basic pieces of equipment in the biopharmaceutical industry. Understanding the effects of mechanical damage and other factors on the physiological state of cells during cell matrix culture is the basis for continuously achieving greater efficiency and higher product quality. In this study, Vero cells were used as a model and apoptosis, senescence, transcriptomics, proteomics, and metabolomics were carried out for analysis at the cellular and molecular levels. The results showed that compared with cells cultured in the simulated natural state, the cells cultured in the basket bioreactor displayed no obvious senescence. Additionally, the proportion of early apoptotic cells increased, but the proportions of damaged, late apoptotic and dead cells did not change significantly. The transcription levels of aminoacyl-tRNA synthetase and cyclin D1 and the expression levels of DNA replication licensing factor, methenyltetrahydrofolate cyclohydrolase, arachidonic acid and other metabolites of cells cultured in the basket bioreactor were significantly increased. These results suggest that DNA replication, protein translation and the metabolic activities in cells cultured in basket bioreactors are more active, which is more conducive to cell amplification and target product production. In this study, the growth and physiological state of cells in a basket bioreactor were characterized at the molecular level for the first time. Additionally, a tool to evaluate the physiological state of cells in a bioreactor was established, which can be used to guide the development and optimization of cell matrix culture conditions in industrial production and improve the production efficiency of the target products. Nature Publishing Group UK 2022-11-23 /pmc/articles/PMC9686226/ /pubmed/36424406 http://dx.doi.org/10.1038/s41598-022-24687-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yu, Shouzhi
Guo, Miaomiao
Zhang, Yadan
Bo, Cunpei
Liang, Hongyang
Wang, Hui
Yang, Xiaoming
Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title_full Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title_fullStr Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title_full_unstemmed Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title_short Comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
title_sort comparative multiomics analysis of cell physiological state after culture in a basket bioreactor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686226/
https://www.ncbi.nlm.nih.gov/pubmed/36424406
http://dx.doi.org/10.1038/s41598-022-24687-4
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