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Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli

Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (A...

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Autores principales: Ul Haq, Shahbaz, Wang, Ling, Guo, Wenzhou, Aqib, Amjad Islam, Muneer, Afshan, Saqib, Muhammad, Ahmad, Saad, Ghafoor, Muzafar, Iftikhar, Amir, Chen, Keyuan, Liang, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686389/
https://www.ncbi.nlm.nih.gov/pubmed/36439355
http://dx.doi.org/10.3389/fvets.2022.1048531
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author Ul Haq, Shahbaz
Wang, Ling
Guo, Wenzhou
Aqib, Amjad Islam
Muneer, Afshan
Saqib, Muhammad
Ahmad, Saad
Ghafoor, Muzafar
Iftikhar, Amir
Chen, Keyuan
Liang, Jianping
author_facet Ul Haq, Shahbaz
Wang, Ling
Guo, Wenzhou
Aqib, Amjad Islam
Muneer, Afshan
Saqib, Muhammad
Ahmad, Saad
Ghafoor, Muzafar
Iftikhar, Amir
Chen, Keyuan
Liang, Jianping
author_sort Ul Haq, Shahbaz
collection PubMed
description Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (AS), and artemisinic acid (AA) with β-lactam and fluoroquinolones antibiotics against Escherichia coli. Antibiotic strip test (E-test), Kirby Bauer's disc test (KB method), and broth microdilution method were adopted for susceptibility analysis, while the checkerboard method was applied to assess synergisms. ART, DHA, AS, and AA showed significantly enhanced antibacterial effects of β-lactam antibiotics against different strains of E. coli. The study showed ciprofloxacin to be most effective by presenting the least MIC (0.017125 ± 0.0022 μg/ml), while oxacillin was least effective (MIC 256 μg/ml) against E. coli. Synergism between AA and penicillin G (75%), ampicillin (25%), and oxacillin (50%) was observed in all isolates tested. AA and AS significantly decreased the MIC of ampicillin (−0.912 ± 0.908 μg/ml) and ciprofloxacin (−0.901 ± 0.893 g/ml), respectively. Artemisinin and its derivatives increased antibiotic accumulation within E. coli in a dose-dependent manner. The time-kill assay significantly reduced the bacterial number within 24 h of incubation. The study thus concludes greater room for improvement in enhancing the efficacy of antibiotics if used with artemisinin and its derivatives.
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spelling pubmed-96863892022-11-25 Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli Ul Haq, Shahbaz Wang, Ling Guo, Wenzhou Aqib, Amjad Islam Muneer, Afshan Saqib, Muhammad Ahmad, Saad Ghafoor, Muzafar Iftikhar, Amir Chen, Keyuan Liang, Jianping Front Vet Sci Veterinary Science Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (AS), and artemisinic acid (AA) with β-lactam and fluoroquinolones antibiotics against Escherichia coli. Antibiotic strip test (E-test), Kirby Bauer's disc test (KB method), and broth microdilution method were adopted for susceptibility analysis, while the checkerboard method was applied to assess synergisms. ART, DHA, AS, and AA showed significantly enhanced antibacterial effects of β-lactam antibiotics against different strains of E. coli. The study showed ciprofloxacin to be most effective by presenting the least MIC (0.017125 ± 0.0022 μg/ml), while oxacillin was least effective (MIC 256 μg/ml) against E. coli. Synergism between AA and penicillin G (75%), ampicillin (25%), and oxacillin (50%) was observed in all isolates tested. AA and AS significantly decreased the MIC of ampicillin (−0.912 ± 0.908 μg/ml) and ciprofloxacin (−0.901 ± 0.893 g/ml), respectively. Artemisinin and its derivatives increased antibiotic accumulation within E. coli in a dose-dependent manner. The time-kill assay significantly reduced the bacterial number within 24 h of incubation. The study thus concludes greater room for improvement in enhancing the efficacy of antibiotics if used with artemisinin and its derivatives. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9686389/ /pubmed/36439355 http://dx.doi.org/10.3389/fvets.2022.1048531 Text en Copyright © 2022 Ul Haq, Wang, Guo, Aqib, Muneer, Saqib, Ahmad, Ghafoor, Iftikhar, Chen and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ul Haq, Shahbaz
Wang, Ling
Guo, Wenzhou
Aqib, Amjad Islam
Muneer, Afshan
Saqib, Muhammad
Ahmad, Saad
Ghafoor, Muzafar
Iftikhar, Amir
Chen, Keyuan
Liang, Jianping
Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title_full Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title_fullStr Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title_full_unstemmed Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title_short Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli
title_sort enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against mdr escherichia coli
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686389/
https://www.ncbi.nlm.nih.gov/pubmed/36439355
http://dx.doi.org/10.3389/fvets.2022.1048531
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