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Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic fo...

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Autores principales: Al-Bakri, Amal G., Dahabiyeh, Lina A., Khalil, Enam, Jaber, Deema, Kamel, Gihan, Schleimer, Nina, Kohler, Christian, Becker, Karsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686486/
https://www.ncbi.nlm.nih.gov/pubmed/36421251
http://dx.doi.org/10.3390/antibiotics11111607
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author Al-Bakri, Amal G.
Dahabiyeh, Lina A.
Khalil, Enam
Jaber, Deema
Kamel, Gihan
Schleimer, Nina
Kohler, Christian
Becker, Karsten
author_facet Al-Bakri, Amal G.
Dahabiyeh, Lina A.
Khalil, Enam
Jaber, Deema
Kamel, Gihan
Schleimer, Nina
Kohler, Christian
Becker, Karsten
author_sort Al-Bakri, Amal G.
collection PubMed
description Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm(−1)), corresponding to the distribution of lipids, and (1855–1500 cm(−1)), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH(2)/CH(3) ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm(−1), assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains.
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spelling pubmed-96864862022-11-25 Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants Al-Bakri, Amal G. Dahabiyeh, Lina A. Khalil, Enam Jaber, Deema Kamel, Gihan Schleimer, Nina Kohler, Christian Becker, Karsten Antibiotics (Basel) Article Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm(−1)), corresponding to the distribution of lipids, and (1855–1500 cm(−1)), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH(2)/CH(3) ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm(−1), assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains. MDPI 2022-11-11 /pmc/articles/PMC9686486/ /pubmed/36421251 http://dx.doi.org/10.3390/antibiotics11111607 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Bakri, Amal G.
Dahabiyeh, Lina A.
Khalil, Enam
Jaber, Deema
Kamel, Gihan
Schleimer, Nina
Kohler, Christian
Becker, Karsten
Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title_full Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title_fullStr Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title_full_unstemmed Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title_short Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants
title_sort synchrotron-radiation-based fourier transform infrared microspectroscopy as a tool for the differentiation between staphylococcal small colony variants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686486/
https://www.ncbi.nlm.nih.gov/pubmed/36421251
http://dx.doi.org/10.3390/antibiotics11111607
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