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Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate

This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid a...

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Autores principales: Fernandes, Gabriela Lopes, Vieira, Ana Paula Miranda, Danelon, Marcelle, Emerenciano, Nayara Gonçalves, Berretta, Andresa Aparecida, Buszinski, Andrei Felipe Moreira, Hori, Juliana Issa, de Lima, Mikhael Haruo Fernandes, dos Reis, Thaila Fernanda, de Lima, Jessica Aparecida, Delbem, Alberto Carlos Botazzo, da Silva, Sónia Carina Morais, Barbosa, Debora Barros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686636/
https://www.ncbi.nlm.nih.gov/pubmed/36358132
http://dx.doi.org/10.3390/antibiotics11111477
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author Fernandes, Gabriela Lopes
Vieira, Ana Paula Miranda
Danelon, Marcelle
Emerenciano, Nayara Gonçalves
Berretta, Andresa Aparecida
Buszinski, Andrei Felipe Moreira
Hori, Juliana Issa
de Lima, Mikhael Haruo Fernandes
dos Reis, Thaila Fernanda
de Lima, Jessica Aparecida
Delbem, Alberto Carlos Botazzo
da Silva, Sónia Carina Morais
Barbosa, Debora Barros
author_facet Fernandes, Gabriela Lopes
Vieira, Ana Paula Miranda
Danelon, Marcelle
Emerenciano, Nayara Gonçalves
Berretta, Andresa Aparecida
Buszinski, Andrei Felipe Moreira
Hori, Juliana Issa
de Lima, Mikhael Haruo Fernandes
dos Reis, Thaila Fernanda
de Lima, Jessica Aparecida
Delbem, Alberto Carlos Botazzo
da Silva, Sónia Carina Morais
Barbosa, Debora Barros
author_sort Fernandes, Gabriela Lopes
collection PubMed
description This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium.
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spelling pubmed-96866362022-11-25 Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate Fernandes, Gabriela Lopes Vieira, Ana Paula Miranda Danelon, Marcelle Emerenciano, Nayara Gonçalves Berretta, Andresa Aparecida Buszinski, Andrei Felipe Moreira Hori, Juliana Issa de Lima, Mikhael Haruo Fernandes dos Reis, Thaila Fernanda de Lima, Jessica Aparecida Delbem, Alberto Carlos Botazzo da Silva, Sónia Carina Morais Barbosa, Debora Barros Antibiotics (Basel) Article This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium. MDPI 2022-10-25 /pmc/articles/PMC9686636/ /pubmed/36358132 http://dx.doi.org/10.3390/antibiotics11111477 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandes, Gabriela Lopes
Vieira, Ana Paula Miranda
Danelon, Marcelle
Emerenciano, Nayara Gonçalves
Berretta, Andresa Aparecida
Buszinski, Andrei Felipe Moreira
Hori, Juliana Issa
de Lima, Mikhael Haruo Fernandes
dos Reis, Thaila Fernanda
de Lima, Jessica Aparecida
Delbem, Alberto Carlos Botazzo
da Silva, Sónia Carina Morais
Barbosa, Debora Barros
Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title_full Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title_fullStr Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title_full_unstemmed Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title_short Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
title_sort pomegranate extract potentiates the anti-demineralizing, anti-biofilm, and anti-inflammatory actions of non-alcoholic mouthwash when associated with sodium-fluoride trimetaphosphate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686636/
https://www.ncbi.nlm.nih.gov/pubmed/36358132
http://dx.doi.org/10.3390/antibiotics11111477
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