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Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate
This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid a...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686636/ https://www.ncbi.nlm.nih.gov/pubmed/36358132 http://dx.doi.org/10.3390/antibiotics11111477 |
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author | Fernandes, Gabriela Lopes Vieira, Ana Paula Miranda Danelon, Marcelle Emerenciano, Nayara Gonçalves Berretta, Andresa Aparecida Buszinski, Andrei Felipe Moreira Hori, Juliana Issa de Lima, Mikhael Haruo Fernandes dos Reis, Thaila Fernanda de Lima, Jessica Aparecida Delbem, Alberto Carlos Botazzo da Silva, Sónia Carina Morais Barbosa, Debora Barros |
author_facet | Fernandes, Gabriela Lopes Vieira, Ana Paula Miranda Danelon, Marcelle Emerenciano, Nayara Gonçalves Berretta, Andresa Aparecida Buszinski, Andrei Felipe Moreira Hori, Juliana Issa de Lima, Mikhael Haruo Fernandes dos Reis, Thaila Fernanda de Lima, Jessica Aparecida Delbem, Alberto Carlos Botazzo da Silva, Sónia Carina Morais Barbosa, Debora Barros |
author_sort | Fernandes, Gabriela Lopes |
collection | PubMed |
description | This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium. |
format | Online Article Text |
id | pubmed-9686636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96866362022-11-25 Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate Fernandes, Gabriela Lopes Vieira, Ana Paula Miranda Danelon, Marcelle Emerenciano, Nayara Gonçalves Berretta, Andresa Aparecida Buszinski, Andrei Felipe Moreira Hori, Juliana Issa de Lima, Mikhael Haruo Fernandes dos Reis, Thaila Fernanda de Lima, Jessica Aparecida Delbem, Alberto Carlos Botazzo da Silva, Sónia Carina Morais Barbosa, Debora Barros Antibiotics (Basel) Article This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium. MDPI 2022-10-25 /pmc/articles/PMC9686636/ /pubmed/36358132 http://dx.doi.org/10.3390/antibiotics11111477 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fernandes, Gabriela Lopes Vieira, Ana Paula Miranda Danelon, Marcelle Emerenciano, Nayara Gonçalves Berretta, Andresa Aparecida Buszinski, Andrei Felipe Moreira Hori, Juliana Issa de Lima, Mikhael Haruo Fernandes dos Reis, Thaila Fernanda de Lima, Jessica Aparecida Delbem, Alberto Carlos Botazzo da Silva, Sónia Carina Morais Barbosa, Debora Barros Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title | Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title_full | Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title_fullStr | Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title_full_unstemmed | Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title_short | Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate |
title_sort | pomegranate extract potentiates the anti-demineralizing, anti-biofilm, and anti-inflammatory actions of non-alcoholic mouthwash when associated with sodium-fluoride trimetaphosphate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686636/ https://www.ncbi.nlm.nih.gov/pubmed/36358132 http://dx.doi.org/10.3390/antibiotics11111477 |
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