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Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model

Bee bread (BB) has traditionally been used as a dietary supplement to treat liver problems. This study evaluated the therapeutic effects of Heterotrigona itama BB from Malaysia on obesity-induced hepatic lipid metabolism disorder via the regulation of the Keap1/Nrf2 pathway. Male Sprague Dawley rats...

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Autores principales: Zakaria, Zaida, Othman, Zaidatul Akmal, Suleiman, Joseph Bagi, Mustaffa, Khairul Mohd Fadzli, Jalil, Nur Asyilla Che, Ghazali, Wan Syaheedah Wan, Zulkipli, Ninie Nadia, Mohamed, Mahaneem, Kamaruzaman, Khaidatul Akmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686663/
https://www.ncbi.nlm.nih.gov/pubmed/36358563
http://dx.doi.org/10.3390/antiox11112190
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author Zakaria, Zaida
Othman, Zaidatul Akmal
Suleiman, Joseph Bagi
Mustaffa, Khairul Mohd Fadzli
Jalil, Nur Asyilla Che
Ghazali, Wan Syaheedah Wan
Zulkipli, Ninie Nadia
Mohamed, Mahaneem
Kamaruzaman, Khaidatul Akmar
author_facet Zakaria, Zaida
Othman, Zaidatul Akmal
Suleiman, Joseph Bagi
Mustaffa, Khairul Mohd Fadzli
Jalil, Nur Asyilla Che
Ghazali, Wan Syaheedah Wan
Zulkipli, Ninie Nadia
Mohamed, Mahaneem
Kamaruzaman, Khaidatul Akmar
author_sort Zakaria, Zaida
collection PubMed
description Bee bread (BB) has traditionally been used as a dietary supplement to treat liver problems. This study evaluated the therapeutic effects of Heterotrigona itama BB from Malaysia on obesity-induced hepatic lipid metabolism disorder via the regulation of the Keap1/Nrf2 pathway. Male Sprague Dawley rats were fed with either a normal diet or high-fat diet (HFD) for 6 weeks to induce obesity. Following 6 weeks, obese rats were treated either with distilled water (OB group), BB (0.5 g/kg body weight/day) (OB + BB group) or orlistat (10 mg/kg body weight/day) (OB + OR group) concurrent with HFD for another 6 weeks. BB treatment suppressed Keap1 and promoted Nrf2 cytoplasmic and nuclear translocations, leading to a reduction in oxidative stress, and promoted antioxidant enzyme activities in the liver. Furthermore, BB down-regulated lipid synthesis and its regulator levels (SIRT1, AMPK), and up-regulated fatty acid β-oxidation in the liver of obese rats, being consistent with alleviated lipid levels, improved hepatic histopathological changes (steatosis, hepatocellular hypertrophy, inflammation and glycogen expression) and prevented progression to non-alcoholic steatohepatitis. These results showed the therapeutic potentials of H. itama BB against oxidative stress and improved lipid metabolism in the liver of obese rats possibly by targeting the Keap1/Nrf2 pathway, hence proposing its role as a natural supplement capable of treating obesity-induced fatty liver disease.
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spelling pubmed-96866632022-11-25 Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model Zakaria, Zaida Othman, Zaidatul Akmal Suleiman, Joseph Bagi Mustaffa, Khairul Mohd Fadzli Jalil, Nur Asyilla Che Ghazali, Wan Syaheedah Wan Zulkipli, Ninie Nadia Mohamed, Mahaneem Kamaruzaman, Khaidatul Akmar Antioxidants (Basel) Article Bee bread (BB) has traditionally been used as a dietary supplement to treat liver problems. This study evaluated the therapeutic effects of Heterotrigona itama BB from Malaysia on obesity-induced hepatic lipid metabolism disorder via the regulation of the Keap1/Nrf2 pathway. Male Sprague Dawley rats were fed with either a normal diet or high-fat diet (HFD) for 6 weeks to induce obesity. Following 6 weeks, obese rats were treated either with distilled water (OB group), BB (0.5 g/kg body weight/day) (OB + BB group) or orlistat (10 mg/kg body weight/day) (OB + OR group) concurrent with HFD for another 6 weeks. BB treatment suppressed Keap1 and promoted Nrf2 cytoplasmic and nuclear translocations, leading to a reduction in oxidative stress, and promoted antioxidant enzyme activities in the liver. Furthermore, BB down-regulated lipid synthesis and its regulator levels (SIRT1, AMPK), and up-regulated fatty acid β-oxidation in the liver of obese rats, being consistent with alleviated lipid levels, improved hepatic histopathological changes (steatosis, hepatocellular hypertrophy, inflammation and glycogen expression) and prevented progression to non-alcoholic steatohepatitis. These results showed the therapeutic potentials of H. itama BB against oxidative stress and improved lipid metabolism in the liver of obese rats possibly by targeting the Keap1/Nrf2 pathway, hence proposing its role as a natural supplement capable of treating obesity-induced fatty liver disease. MDPI 2022-11-05 /pmc/articles/PMC9686663/ /pubmed/36358563 http://dx.doi.org/10.3390/antiox11112190 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zakaria, Zaida
Othman, Zaidatul Akmal
Suleiman, Joseph Bagi
Mustaffa, Khairul Mohd Fadzli
Jalil, Nur Asyilla Che
Ghazali, Wan Syaheedah Wan
Zulkipli, Ninie Nadia
Mohamed, Mahaneem
Kamaruzaman, Khaidatul Akmar
Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title_full Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title_fullStr Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title_full_unstemmed Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title_short Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through the Activation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
title_sort therapeutic effects of heterotrigona itama (stingless bee) bee bread in improving hepatic lipid metabolism through the activation of the keap1/nrf2 signaling pathway in an obese rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686663/
https://www.ncbi.nlm.nih.gov/pubmed/36358563
http://dx.doi.org/10.3390/antiox11112190
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