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Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

Nano-drug delivery is a promising tactic to enhance the activity and minimize the cytotoxicity of antimicrobial drugs. In the current study, chitosan nanoparticles (CSNPs) were used as a carrier for the delivery of gentamicin sulfate (GM) and ascorbic acid (AA). The particles were synthesized by ion...

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Autores principales: Abdel-Hakeem, Mohamed A., Abdel Maksoud, Ahmed I., Aladhadh, Mohammed Abdullah, Almuryif, Khalid Abdulrahman, Elsanhoty, Rafaat M., Elebeedy, Dalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686670/
https://www.ncbi.nlm.nih.gov/pubmed/36358185
http://dx.doi.org/10.3390/antibiotics11111530
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author Abdel-Hakeem, Mohamed A.
Abdel Maksoud, Ahmed I.
Aladhadh, Mohammed Abdullah
Almuryif, Khalid Abdulrahman
Elsanhoty, Rafaat M.
Elebeedy, Dalia
author_facet Abdel-Hakeem, Mohamed A.
Abdel Maksoud, Ahmed I.
Aladhadh, Mohammed Abdullah
Almuryif, Khalid Abdulrahman
Elsanhoty, Rafaat M.
Elebeedy, Dalia
author_sort Abdel-Hakeem, Mohamed A.
collection PubMed
description Nano-drug delivery is a promising tactic to enhance the activity and minimize the cytotoxicity of antimicrobial drugs. In the current study, chitosan nanoparticles (CSNPs) were used as a carrier for the delivery of gentamicin sulfate (GM) and ascorbic acid (AA). The particles were synthesized by ionotropic gelation method and characterized by FT-IR, Zeta potential, and transmission electron microscope imaging. The obtained particles were evaluated for their in vitro antimicrobial activity and cytotoxicity. The prepared particles (GM–AA–CSNPs) under the optimal condition of 4:1:1 of chitosan to drug ratio showed encapsulation efficiency and loading capacities of 89% and 22%, respectively. Regarding biological activities, GM–AA–CSNPs showed a lower minimum inhibitory concentration (MIC) than free gentamicin sulfate and GMCSNPs mixture without presenting cytotoxicity against normal cells (HSF). Moreover, the GM–AA–CSNPs did not exhibit hemolytic activity. These results highlight that the GM–AA–CSNPs are confirmed as a hopeful formula for future investigations on the development of antimicrobial preparations.
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spelling pubmed-96866702022-11-25 Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity Abdel-Hakeem, Mohamed A. Abdel Maksoud, Ahmed I. Aladhadh, Mohammed Abdullah Almuryif, Khalid Abdulrahman Elsanhoty, Rafaat M. Elebeedy, Dalia Antibiotics (Basel) Article Nano-drug delivery is a promising tactic to enhance the activity and minimize the cytotoxicity of antimicrobial drugs. In the current study, chitosan nanoparticles (CSNPs) were used as a carrier for the delivery of gentamicin sulfate (GM) and ascorbic acid (AA). The particles were synthesized by ionotropic gelation method and characterized by FT-IR, Zeta potential, and transmission electron microscope imaging. The obtained particles were evaluated for their in vitro antimicrobial activity and cytotoxicity. The prepared particles (GM–AA–CSNPs) under the optimal condition of 4:1:1 of chitosan to drug ratio showed encapsulation efficiency and loading capacities of 89% and 22%, respectively. Regarding biological activities, GM–AA–CSNPs showed a lower minimum inhibitory concentration (MIC) than free gentamicin sulfate and GMCSNPs mixture without presenting cytotoxicity against normal cells (HSF). Moreover, the GM–AA–CSNPs did not exhibit hemolytic activity. These results highlight that the GM–AA–CSNPs are confirmed as a hopeful formula for future investigations on the development of antimicrobial preparations. MDPI 2022-11-02 /pmc/articles/PMC9686670/ /pubmed/36358185 http://dx.doi.org/10.3390/antibiotics11111530 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdel-Hakeem, Mohamed A.
Abdel Maksoud, Ahmed I.
Aladhadh, Mohammed Abdullah
Almuryif, Khalid Abdulrahman
Elsanhoty, Rafaat M.
Elebeedy, Dalia
Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title_full Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title_fullStr Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title_full_unstemmed Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title_short Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity
title_sort gentamicin–ascorbic acid encapsulated in chitosan nanoparticles improved in vitro antimicrobial activity and minimized cytotoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686670/
https://www.ncbi.nlm.nih.gov/pubmed/36358185
http://dx.doi.org/10.3390/antibiotics11111530
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