Antioxidant Potential of Resveratrol as the Result of Radiation Exposition

The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the co...

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Autores principales: Rosiak, Natalia, Cielecka-Piontek, Judyta, Skibiński, Robert, Lewandowska, Kornelia, Bednarski, Waldemar, Zalewski, Przemysław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686677/
https://www.ncbi.nlm.nih.gov/pubmed/36358469
http://dx.doi.org/10.3390/antiox11112097
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author Rosiak, Natalia
Cielecka-Piontek, Judyta
Skibiński, Robert
Lewandowska, Kornelia
Bednarski, Waldemar
Zalewski, Przemysław
author_facet Rosiak, Natalia
Cielecka-Piontek, Judyta
Skibiński, Robert
Lewandowska, Kornelia
Bednarski, Waldemar
Zalewski, Przemysław
author_sort Rosiak, Natalia
collection PubMed
description The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the concentration of free radicals after irradiation. Minor changes in chemical structure due to free radicals induced by EBI were confirmed by FTIR spectroscopy. HPLC and HPLC-MS analysis ruled out the appearance of degradation products after irradiation. In addition, HPLC analysis confirmed the absence of trans- to cis-resveratrol conversion. Changes in the antioxidant potential of RSV after irradiation were studied using DPPH, ABTS, CUPRAC, and FRAP techniques. It was confirmed that EBI favorably affected the antioxidant properties of tests based on the HAT mechanism (increase in DPPH and CUPRAC tests).
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spelling pubmed-96866772022-11-25 Antioxidant Potential of Resveratrol as the Result of Radiation Exposition Rosiak, Natalia Cielecka-Piontek, Judyta Skibiński, Robert Lewandowska, Kornelia Bednarski, Waldemar Zalewski, Przemysław Antioxidants (Basel) Article The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the concentration of free radicals after irradiation. Minor changes in chemical structure due to free radicals induced by EBI were confirmed by FTIR spectroscopy. HPLC and HPLC-MS analysis ruled out the appearance of degradation products after irradiation. In addition, HPLC analysis confirmed the absence of trans- to cis-resveratrol conversion. Changes in the antioxidant potential of RSV after irradiation were studied using DPPH, ABTS, CUPRAC, and FRAP techniques. It was confirmed that EBI favorably affected the antioxidant properties of tests based on the HAT mechanism (increase in DPPH and CUPRAC tests). MDPI 2022-10-24 /pmc/articles/PMC9686677/ /pubmed/36358469 http://dx.doi.org/10.3390/antiox11112097 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosiak, Natalia
Cielecka-Piontek, Judyta
Skibiński, Robert
Lewandowska, Kornelia
Bednarski, Waldemar
Zalewski, Przemysław
Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title_full Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title_fullStr Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title_full_unstemmed Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title_short Antioxidant Potential of Resveratrol as the Result of Radiation Exposition
title_sort antioxidant potential of resveratrol as the result of radiation exposition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686677/
https://www.ncbi.nlm.nih.gov/pubmed/36358469
http://dx.doi.org/10.3390/antiox11112097
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