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Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China

SIMPLE SUMMARY: This study investigated the family structure, parent–child pair and inbreeding coefficient of a Tan sheep breeding farm in Ningxia, China. The results can be used as the theoretical basis for selecting excellent breeding stock and making an apposite mating plan; furthermore, it can p...

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Autores principales: Li, Ling, Li, Yefang, Ma, Qing, Liu, Shuqin, Ma, Yuehui, Jiang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686711/
https://www.ncbi.nlm.nih.gov/pubmed/36428327
http://dx.doi.org/10.3390/ani12223099
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author Li, Ling
Li, Yefang
Ma, Qing
Liu, Shuqin
Ma, Yuehui
Jiang, Lin
author_facet Li, Ling
Li, Yefang
Ma, Qing
Liu, Shuqin
Ma, Yuehui
Jiang, Lin
author_sort Li, Ling
collection PubMed
description SIMPLE SUMMARY: This study investigated the family structure, parent–child pair and inbreeding coefficient of a Tan sheep breeding farm in Ningxia, China. The results can be used as the theoretical basis for selecting excellent breeding stock and making an apposite mating plan; furthermore, it can provide an important scientific basis for the conservation and reproduction of Tan sheep germplasm resources. ABSTRACT: Tan sheep is a special breed of locally protected sheep in China, one of the best quality meat sheep in the world. Due to the unclear pedigree of the rams on the Ningxia Tan sheep breeding farm, we investigated 74 rams in the field and explored a new method for family division. Genomic DNA was extracted from the blood of breeding rams. Using Plink software, GCTA tools and R language, we analyzed the genetic structure, kinship, and inbreeding coefficient of the breeding sheep, which revealed the genetic relationship between the individuals. The results showed that there was no obvious clustering phenomenon in the PCA, and the genetic background of the samples was similar. The G matrix and IBS distance matrix indicated that most individuals were far away from each other. Paternity testing identified 24 pairs of unknown parent–child pairs, and all the Tan sheep could be divided into 12 families, which provided a reference for sheep breeding. The average inbreeding coefficient based on the ROH of this population was 0.049, so there was a low degree of inbreeding and the rams in the field were able to maintain high genetic diversity. Overall, we explored a more accurate method through paternity and kinship analysis; it provides a scientific basis for pedigree construction, which has an important application value for Tan sheep breeding.
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spelling pubmed-96867112022-11-25 Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China Li, Ling Li, Yefang Ma, Qing Liu, Shuqin Ma, Yuehui Jiang, Lin Animals (Basel) Article SIMPLE SUMMARY: This study investigated the family structure, parent–child pair and inbreeding coefficient of a Tan sheep breeding farm in Ningxia, China. The results can be used as the theoretical basis for selecting excellent breeding stock and making an apposite mating plan; furthermore, it can provide an important scientific basis for the conservation and reproduction of Tan sheep germplasm resources. ABSTRACT: Tan sheep is a special breed of locally protected sheep in China, one of the best quality meat sheep in the world. Due to the unclear pedigree of the rams on the Ningxia Tan sheep breeding farm, we investigated 74 rams in the field and explored a new method for family division. Genomic DNA was extracted from the blood of breeding rams. Using Plink software, GCTA tools and R language, we analyzed the genetic structure, kinship, and inbreeding coefficient of the breeding sheep, which revealed the genetic relationship between the individuals. The results showed that there was no obvious clustering phenomenon in the PCA, and the genetic background of the samples was similar. The G matrix and IBS distance matrix indicated that most individuals were far away from each other. Paternity testing identified 24 pairs of unknown parent–child pairs, and all the Tan sheep could be divided into 12 families, which provided a reference for sheep breeding. The average inbreeding coefficient based on the ROH of this population was 0.049, so there was a low degree of inbreeding and the rams in the field were able to maintain high genetic diversity. Overall, we explored a more accurate method through paternity and kinship analysis; it provides a scientific basis for pedigree construction, which has an important application value for Tan sheep breeding. MDPI 2022-11-10 /pmc/articles/PMC9686711/ /pubmed/36428327 http://dx.doi.org/10.3390/ani12223099 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Ling
Li, Yefang
Ma, Qing
Liu, Shuqin
Ma, Yuehui
Jiang, Lin
Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title_full Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title_fullStr Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title_full_unstemmed Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title_short Analysis of Family Structure and Paternity Test of Tan Sheep in Yanchi Area, China
title_sort analysis of family structure and paternity test of tan sheep in yanchi area, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686711/
https://www.ncbi.nlm.nih.gov/pubmed/36428327
http://dx.doi.org/10.3390/ani12223099
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