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Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat

Background: Widespread use of antibiotics as growth promoters and prophylactic agents has dramatic consequences for the development of antibiotic resistance. In this study, we investigated effects of selected antibiotics on bacterial biofilms and performed extensive antibiotic and VF profiling of po...

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Autores principales: Noreen, Aisha, Masood, Hamid, Zaib, Jaweria, Rafaque, Zara, Fatima, Areeta, Shabbir, Hira, Alam, Javaria, Habib, Aisha, Noor, Saba, Dil, Kinza, Dasti, Javid Iqbal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686770/
https://www.ncbi.nlm.nih.gov/pubmed/36421307
http://dx.doi.org/10.3390/antibiotics11111663
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author Noreen, Aisha
Masood, Hamid
Zaib, Jaweria
Rafaque, Zara
Fatima, Areeta
Shabbir, Hira
Alam, Javaria
Habib, Aisha
Noor, Saba
Dil, Kinza
Dasti, Javid Iqbal
author_facet Noreen, Aisha
Masood, Hamid
Zaib, Jaweria
Rafaque, Zara
Fatima, Areeta
Shabbir, Hira
Alam, Javaria
Habib, Aisha
Noor, Saba
Dil, Kinza
Dasti, Javid Iqbal
author_sort Noreen, Aisha
collection PubMed
description Background: Widespread use of antibiotics as growth promoters and prophylactic agents has dramatic consequences for the development of antibiotic resistance. In this study, we investigated effects of selected antibiotics on bacterial biofilms and performed extensive antibiotic and VF profiling of poultry-meat associated E. coli strains. Methods: Antibiotic susceptibility was performed by a disc diffusion method, followed by molecular screening of resistance and virulence determinants. Further biofilm formation assays, MIC-p, MIC-b, MBIC and MBEC, were performed using standard tissue culture plate method. Results: In total, 83 (75%) samples were confirmed as E. coli from poultry sources, 26 different antibiotics were tested, and maximum numbers of the isolates were resistant to lincomycin (100%), while the least resistance was seen against cefotaxime (1%) and polymyxin B (1%). Overall, 48% of the isolates were ESBL producers and 40% showed carbapenemase activity; important virulence genes were detected in following percentages: fimH32 (39%), papC21 (25%), iutA34 (41%), kpsMT-II23 (28%), papEF9 (11%), papGII22 (27%) and fyuA13 (16%). Colistin showed remarkable anti-biofilm activity, while at sub-MIC levels, gentamicin, ceftriaxone and enrofloxin significantly (p < 0.01) inhibited the biofilms. A strong induction of bacterial biofilm, after exposure to sub-minimal levels of colistin clearly indicates risk of bacterial overgrowth in a farm environment, while use of colistin aggravates the risk of emergence of colistin resistant Enterobacteriaceae, a highly undesirable public health scenario.
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spelling pubmed-96867702022-11-25 Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat Noreen, Aisha Masood, Hamid Zaib, Jaweria Rafaque, Zara Fatima, Areeta Shabbir, Hira Alam, Javaria Habib, Aisha Noor, Saba Dil, Kinza Dasti, Javid Iqbal Antibiotics (Basel) Article Background: Widespread use of antibiotics as growth promoters and prophylactic agents has dramatic consequences for the development of antibiotic resistance. In this study, we investigated effects of selected antibiotics on bacterial biofilms and performed extensive antibiotic and VF profiling of poultry-meat associated E. coli strains. Methods: Antibiotic susceptibility was performed by a disc diffusion method, followed by molecular screening of resistance and virulence determinants. Further biofilm formation assays, MIC-p, MIC-b, MBIC and MBEC, were performed using standard tissue culture plate method. Results: In total, 83 (75%) samples were confirmed as E. coli from poultry sources, 26 different antibiotics were tested, and maximum numbers of the isolates were resistant to lincomycin (100%), while the least resistance was seen against cefotaxime (1%) and polymyxin B (1%). Overall, 48% of the isolates were ESBL producers and 40% showed carbapenemase activity; important virulence genes were detected in following percentages: fimH32 (39%), papC21 (25%), iutA34 (41%), kpsMT-II23 (28%), papEF9 (11%), papGII22 (27%) and fyuA13 (16%). Colistin showed remarkable anti-biofilm activity, while at sub-MIC levels, gentamicin, ceftriaxone and enrofloxin significantly (p < 0.01) inhibited the biofilms. A strong induction of bacterial biofilm, after exposure to sub-minimal levels of colistin clearly indicates risk of bacterial overgrowth in a farm environment, while use of colistin aggravates the risk of emergence of colistin resistant Enterobacteriaceae, a highly undesirable public health scenario. MDPI 2022-11-19 /pmc/articles/PMC9686770/ /pubmed/36421307 http://dx.doi.org/10.3390/antibiotics11111663 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Noreen, Aisha
Masood, Hamid
Zaib, Jaweria
Rafaque, Zara
Fatima, Areeta
Shabbir, Hira
Alam, Javaria
Habib, Aisha
Noor, Saba
Dil, Kinza
Dasti, Javid Iqbal
Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title_full Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title_fullStr Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title_full_unstemmed Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title_short Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat
title_sort investigating the role of antibiotics on induction, inhibition and eradication of biofilms of poultry associated escherichia coli isolated from retail chicken meat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686770/
https://www.ncbi.nlm.nih.gov/pubmed/36421307
http://dx.doi.org/10.3390/antibiotics11111663
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