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Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities

At present, multidrug-resistant microorganisms are already responsible for community-acquired infections. Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious public health risk worldwide because of the rapid spread and diversification of pandemic clones that are characterized by incre...

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Autores principales: Silva, Lucas Porangaba, Fortaleza, Carlos Magno Castelo Branco, Teixeira, Nathalia Bibiana, Silva, Luís Thadeo Poianas, de Angelis, Carolina Destro, Ribeiro de Souza da Cunha, Maria de Lourdes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686811/
https://www.ncbi.nlm.nih.gov/pubmed/36358181
http://dx.doi.org/10.3390/antibiotics11111526
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author Silva, Lucas Porangaba
Fortaleza, Carlos Magno Castelo Branco
Teixeira, Nathalia Bibiana
Silva, Luís Thadeo Poianas
de Angelis, Carolina Destro
Ribeiro de Souza da Cunha, Maria de Lourdes
author_facet Silva, Lucas Porangaba
Fortaleza, Carlos Magno Castelo Branco
Teixeira, Nathalia Bibiana
Silva, Luís Thadeo Poianas
de Angelis, Carolina Destro
Ribeiro de Souza da Cunha, Maria de Lourdes
author_sort Silva, Lucas Porangaba
collection PubMed
description At present, multidrug-resistant microorganisms are already responsible for community-acquired infections. Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious public health risk worldwide because of the rapid spread and diversification of pandemic clones that are characterized by increasing virulence and antimicrobial resistance. The aim of this study was to identify the prevalence and factors associated with nasal, oral and rectal carriage of S. aureus and MRSA in bedridden patients and residents of long-term care facilities for the elderly (LTCFs) in Botucatu, SP, Brazil. Nasal, oral and rectal swab isolates obtained from 226 LTCF residents or home-bedridden patients between 2017 and 2018 were submitted to susceptibility testing, detection of the mecA gene, SCCmec characterization, and molecular typing by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Logistic regression analysis was used to identify risk factors associated with the presence of S. aureus and MRSA. The prevalence of S. aureus and MRSA was 33.6% (n = 76) and 8% (n = 18), respectively. At the nine LTCFs studied, the prevalence of S. aureus ranged from 16.6% to 85.7% and that of MRSA from 13.3% to 25%. Living in an LTCF, male gender, a history of surgeries, and a high Charlson Comorbidity Index score were risk factors associated with S. aureus carriage, while MRSA carriage was positively associated with male gender. This study showed a high prevalence of S. aureus among elderly residents of small (<15 residents) and medium-sized (15–49 residents) LTCFs and a higher prevalence of MRSA in the oropharynx.
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spelling pubmed-96868112022-11-25 Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities Silva, Lucas Porangaba Fortaleza, Carlos Magno Castelo Branco Teixeira, Nathalia Bibiana Silva, Luís Thadeo Poianas de Angelis, Carolina Destro Ribeiro de Souza da Cunha, Maria de Lourdes Antibiotics (Basel) Article At present, multidrug-resistant microorganisms are already responsible for community-acquired infections. Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious public health risk worldwide because of the rapid spread and diversification of pandemic clones that are characterized by increasing virulence and antimicrobial resistance. The aim of this study was to identify the prevalence and factors associated with nasal, oral and rectal carriage of S. aureus and MRSA in bedridden patients and residents of long-term care facilities for the elderly (LTCFs) in Botucatu, SP, Brazil. Nasal, oral and rectal swab isolates obtained from 226 LTCF residents or home-bedridden patients between 2017 and 2018 were submitted to susceptibility testing, detection of the mecA gene, SCCmec characterization, and molecular typing by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Logistic regression analysis was used to identify risk factors associated with the presence of S. aureus and MRSA. The prevalence of S. aureus and MRSA was 33.6% (n = 76) and 8% (n = 18), respectively. At the nine LTCFs studied, the prevalence of S. aureus ranged from 16.6% to 85.7% and that of MRSA from 13.3% to 25%. Living in an LTCF, male gender, a history of surgeries, and a high Charlson Comorbidity Index score were risk factors associated with S. aureus carriage, while MRSA carriage was positively associated with male gender. This study showed a high prevalence of S. aureus among elderly residents of small (<15 residents) and medium-sized (15–49 residents) LTCFs and a higher prevalence of MRSA in the oropharynx. MDPI 2022-11-01 /pmc/articles/PMC9686811/ /pubmed/36358181 http://dx.doi.org/10.3390/antibiotics11111526 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Lucas Porangaba
Fortaleza, Carlos Magno Castelo Branco
Teixeira, Nathalia Bibiana
Silva, Luís Thadeo Poianas
de Angelis, Carolina Destro
Ribeiro de Souza da Cunha, Maria de Lourdes
Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title_full Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title_fullStr Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title_full_unstemmed Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title_short Molecular Epidemiology of Staphylococcus aureus and MRSA in Bedridden Patients and Residents of Long-Term Care Facilities
title_sort molecular epidemiology of staphylococcus aureus and mrsa in bedridden patients and residents of long-term care facilities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686811/
https://www.ncbi.nlm.nih.gov/pubmed/36358181
http://dx.doi.org/10.3390/antibiotics11111526
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