Cargando…
A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes
IncL/M broad-host-range conjugative plasmids are involved in the global spread of bla(OXA-48) and the emergence of bla(NDM-1). The aim of this study was to evaluate the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The conjugation rate...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686884/ https://www.ncbi.nlm.nih.gov/pubmed/36421285 http://dx.doi.org/10.3390/antibiotics11111641 |
_version_ | 1784835865333202944 |
---|---|
author | Getino, María López-Díaz, María Ellaby, Nicholas Clark, John Ellington, Matthew J. La Ragione, Roberto M. |
author_facet | Getino, María López-Díaz, María Ellaby, Nicholas Clark, John Ellington, Matthew J. La Ragione, Roberto M. |
author_sort | Getino, María |
collection | PubMed |
description | IncL/M broad-host-range conjugative plasmids are involved in the global spread of bla(OXA-48) and the emergence of bla(NDM-1). The aim of this study was to evaluate the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The conjugation rate and fitness cost of IncM2 and IncL plasmids encoding these carbapenemase genes were tested using a variety of host bacteria. Genomic analysis of uropathogenic Escherichia coli SAP1756 revealed that bla(NDM-1) was encoded on an IncM2 plasmid, which also harboured bla(TEM-1), ble(MBL) and sul1 and was highly similar to plasmids isolated from the same geographical area. Conjugation experiments demonstrated that NDM-1 and OXA-48-carrying plasmids transfer successfully between different Enterobacterales species, both in vitro and in vivo. Interestingly, E. coli isolates tested as recipients belonging to phylogroups A, B1, D and F were able to receive IncM2 plasmid pSAP1756, while phylogroups B2, C, E and G were not permissive to its acquisition. In general, the IncL OXA-48-carrying plasmids tested transferred at higher rates than IncM2 harbouring NDM-1 and imposed a lower burden to their host, possibly due to the inactivation of the tir fertility inhibition gene and reflecting their worldwide dissemination. IncM2 plasmids carrying bla(NDM-1) are considered emergent threats that need continuous monitoring. In addition to sequencing efforts, phenotypic analysis of conjugation rates and fitness cost are effective methods for estimating the pandemic potential of antimicrobial resistance plasmids. |
format | Online Article Text |
id | pubmed-9686884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96868842022-11-25 A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes Getino, María López-Díaz, María Ellaby, Nicholas Clark, John Ellington, Matthew J. La Ragione, Roberto M. Antibiotics (Basel) Communication IncL/M broad-host-range conjugative plasmids are involved in the global spread of bla(OXA-48) and the emergence of bla(NDM-1). The aim of this study was to evaluate the transmission potential of plasmids encoding the emergent NDM-1 carbapenemase compared to the pandemic OXA-48. The conjugation rate and fitness cost of IncM2 and IncL plasmids encoding these carbapenemase genes were tested using a variety of host bacteria. Genomic analysis of uropathogenic Escherichia coli SAP1756 revealed that bla(NDM-1) was encoded on an IncM2 plasmid, which also harboured bla(TEM-1), ble(MBL) and sul1 and was highly similar to plasmids isolated from the same geographical area. Conjugation experiments demonstrated that NDM-1 and OXA-48-carrying plasmids transfer successfully between different Enterobacterales species, both in vitro and in vivo. Interestingly, E. coli isolates tested as recipients belonging to phylogroups A, B1, D and F were able to receive IncM2 plasmid pSAP1756, while phylogroups B2, C, E and G were not permissive to its acquisition. In general, the IncL OXA-48-carrying plasmids tested transferred at higher rates than IncM2 harbouring NDM-1 and imposed a lower burden to their host, possibly due to the inactivation of the tir fertility inhibition gene and reflecting their worldwide dissemination. IncM2 plasmids carrying bla(NDM-1) are considered emergent threats that need continuous monitoring. In addition to sequencing efforts, phenotypic analysis of conjugation rates and fitness cost are effective methods for estimating the pandemic potential of antimicrobial resistance plasmids. MDPI 2022-11-17 /pmc/articles/PMC9686884/ /pubmed/36421285 http://dx.doi.org/10.3390/antibiotics11111641 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Getino, María López-Díaz, María Ellaby, Nicholas Clark, John Ellington, Matthew J. La Ragione, Roberto M. A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title | A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title_full | A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title_fullStr | A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title_full_unstemmed | A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title_short | A Broad-Host-Range Plasmid Outbreak: Dynamics of IncL/M Plasmids Transferring Carbapenemase Genes |
title_sort | broad-host-range plasmid outbreak: dynamics of incl/m plasmids transferring carbapenemase genes |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686884/ https://www.ncbi.nlm.nih.gov/pubmed/36421285 http://dx.doi.org/10.3390/antibiotics11111641 |
work_keys_str_mv | AT getinomaria abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT lopezdiazmaria abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT ellabynicholas abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT clarkjohn abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT ellingtonmatthewj abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT laragionerobertom abroadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT getinomaria broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT lopezdiazmaria broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT ellabynicholas broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT clarkjohn broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT ellingtonmatthewj broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes AT laragionerobertom broadhostrangeplasmidoutbreakdynamicsofinclmplasmidstransferringcarbapenemasegenes |