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Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn

The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antim...

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Autores principales: Hernández-Martínez, Marco Antonio, Suárez-Rodríguez, Luis María, López-Meza, Joel Edmundo, Ochoa-Zarzosa, Alejandra, Salgado-Garciglia, Rafael, Fernández-Pavia, Silvia Patricia, López-Gómez, Rodolfo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686948/
https://www.ncbi.nlm.nih.gov/pubmed/36358213
http://dx.doi.org/10.3390/antibiotics11111558
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author Hernández-Martínez, Marco Antonio
Suárez-Rodríguez, Luis María
López-Meza, Joel Edmundo
Ochoa-Zarzosa, Alejandra
Salgado-Garciglia, Rafael
Fernández-Pavia, Silvia Patricia
López-Gómez, Rodolfo
author_facet Hernández-Martínez, Marco Antonio
Suárez-Rodríguez, Luis María
López-Meza, Joel Edmundo
Ochoa-Zarzosa, Alejandra
Salgado-Garciglia, Rafael
Fernández-Pavia, Silvia Patricia
López-Gómez, Rodolfo
author_sort Hernández-Martínez, Marco Antonio
collection PubMed
description The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antimicrobial peptide (GASA/Snakin), and demonstrated its antibacterial activity. In this work, we report the recombinant production of PaSn in the Escherichia coli system and evaluate its antifungal activity against plant and human pathogen fungi. The recombinant peptide showed antifungal activity at 200 μg/mL against phytopathogens Colletotrichum gloeosporioides and Fusarium oxysporum and human pathogens Candida albicans and C. glabrata. Our results demonstrate the usefulness of a prokaryotic expression system for avocado antimicrobial peptide production. In conclusion, the snakin PaSn could be helpful in the control of postharvest avocado and other fruits’ fungal diseases and human fungal pathogens.
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spelling pubmed-96869482022-11-25 Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn Hernández-Martínez, Marco Antonio Suárez-Rodríguez, Luis María López-Meza, Joel Edmundo Ochoa-Zarzosa, Alejandra Salgado-Garciglia, Rafael Fernández-Pavia, Silvia Patricia López-Gómez, Rodolfo Antibiotics (Basel) Article The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antimicrobial peptide (GASA/Snakin), and demonstrated its antibacterial activity. In this work, we report the recombinant production of PaSn in the Escherichia coli system and evaluate its antifungal activity against plant and human pathogen fungi. The recombinant peptide showed antifungal activity at 200 μg/mL against phytopathogens Colletotrichum gloeosporioides and Fusarium oxysporum and human pathogens Candida albicans and C. glabrata. Our results demonstrate the usefulness of a prokaryotic expression system for avocado antimicrobial peptide production. In conclusion, the snakin PaSn could be helpful in the control of postharvest avocado and other fruits’ fungal diseases and human fungal pathogens. MDPI 2022-11-05 /pmc/articles/PMC9686948/ /pubmed/36358213 http://dx.doi.org/10.3390/antibiotics11111558 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Martínez, Marco Antonio
Suárez-Rodríguez, Luis María
López-Meza, Joel Edmundo
Ochoa-Zarzosa, Alejandra
Salgado-Garciglia, Rafael
Fernández-Pavia, Silvia Patricia
López-Gómez, Rodolfo
Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title_full Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title_fullStr Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title_full_unstemmed Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title_short Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
title_sort antifungal activity of avocado seed recombinant gasa/snakin pasn
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686948/
https://www.ncbi.nlm.nih.gov/pubmed/36358213
http://dx.doi.org/10.3390/antibiotics11111558
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