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Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn
The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antim...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686948/ https://www.ncbi.nlm.nih.gov/pubmed/36358213 http://dx.doi.org/10.3390/antibiotics11111558 |
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author | Hernández-Martínez, Marco Antonio Suárez-Rodríguez, Luis María López-Meza, Joel Edmundo Ochoa-Zarzosa, Alejandra Salgado-Garciglia, Rafael Fernández-Pavia, Silvia Patricia López-Gómez, Rodolfo |
author_facet | Hernández-Martínez, Marco Antonio Suárez-Rodríguez, Luis María López-Meza, Joel Edmundo Ochoa-Zarzosa, Alejandra Salgado-Garciglia, Rafael Fernández-Pavia, Silvia Patricia López-Gómez, Rodolfo |
author_sort | Hernández-Martínez, Marco Antonio |
collection | PubMed |
description | The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antimicrobial peptide (GASA/Snakin), and demonstrated its antibacterial activity. In this work, we report the recombinant production of PaSn in the Escherichia coli system and evaluate its antifungal activity against plant and human pathogen fungi. The recombinant peptide showed antifungal activity at 200 μg/mL against phytopathogens Colletotrichum gloeosporioides and Fusarium oxysporum and human pathogens Candida albicans and C. glabrata. Our results demonstrate the usefulness of a prokaryotic expression system for avocado antimicrobial peptide production. In conclusion, the snakin PaSn could be helpful in the control of postharvest avocado and other fruits’ fungal diseases and human fungal pathogens. |
format | Online Article Text |
id | pubmed-9686948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96869482022-11-25 Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn Hernández-Martínez, Marco Antonio Suárez-Rodríguez, Luis María López-Meza, Joel Edmundo Ochoa-Zarzosa, Alejandra Salgado-Garciglia, Rafael Fernández-Pavia, Silvia Patricia López-Gómez, Rodolfo Antibiotics (Basel) Article The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antimicrobial peptide (GASA/Snakin), and demonstrated its antibacterial activity. In this work, we report the recombinant production of PaSn in the Escherichia coli system and evaluate its antifungal activity against plant and human pathogen fungi. The recombinant peptide showed antifungal activity at 200 μg/mL against phytopathogens Colletotrichum gloeosporioides and Fusarium oxysporum and human pathogens Candida albicans and C. glabrata. Our results demonstrate the usefulness of a prokaryotic expression system for avocado antimicrobial peptide production. In conclusion, the snakin PaSn could be helpful in the control of postharvest avocado and other fruits’ fungal diseases and human fungal pathogens. MDPI 2022-11-05 /pmc/articles/PMC9686948/ /pubmed/36358213 http://dx.doi.org/10.3390/antibiotics11111558 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernández-Martínez, Marco Antonio Suárez-Rodríguez, Luis María López-Meza, Joel Edmundo Ochoa-Zarzosa, Alejandra Salgado-Garciglia, Rafael Fernández-Pavia, Silvia Patricia López-Gómez, Rodolfo Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title | Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title_full | Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title_fullStr | Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title_full_unstemmed | Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title_short | Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn |
title_sort | antifungal activity of avocado seed recombinant gasa/snakin pasn |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9686948/ https://www.ncbi.nlm.nih.gov/pubmed/36358213 http://dx.doi.org/10.3390/antibiotics11111558 |
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