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Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries

Compartmental boundaries physically separate developing tissues into distinct regions, which is fundamental for the organisation of the body plan in both insects and vertebrates. In many examples, this physical segregation is caused by a regulated increase in contractility of the actomyosin cortex a...

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Autores principales: Sharrock, Thomas E., Evans, Jenny, Blanchard, Guy B., Sanson, Bénédicte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687003/
https://www.ncbi.nlm.nih.gov/pubmed/36178136
http://dx.doi.org/10.1242/dev.200292
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author Sharrock, Thomas E.
Evans, Jenny
Blanchard, Guy B.
Sanson, Bénédicte
author_facet Sharrock, Thomas E.
Evans, Jenny
Blanchard, Guy B.
Sanson, Bénédicte
author_sort Sharrock, Thomas E.
collection PubMed
description Compartmental boundaries physically separate developing tissues into distinct regions, which is fundamental for the organisation of the body plan in both insects and vertebrates. In many examples, this physical segregation is caused by a regulated increase in contractility of the actomyosin cortex at boundary cell-cell interfaces, a property important in developmental morphogenesis beyond compartmental boundary formation. We performed an unbiased screening approach to identify cell surface receptors required for actomyosin enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, from the start of germband extension at gastrulation and throughout the germband extended stages (stages 6 to 11). First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs, confirming an earlier report. Next, by following in real time the dynamics of loss of boundary straightness in tartan mutant embryos compared with wild-type and ftz mutant embryos, we show that Tartan is required during germband extension but not beyond. We identify candidate genes that could take over from Tartan at PSBs and confirm that at germband extended stages, actomyosin enrichment at PSBs requires Wingless signalling.
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spelling pubmed-96870032022-12-16 Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries Sharrock, Thomas E. Evans, Jenny Blanchard, Guy B. Sanson, Bénédicte Development Research Article Compartmental boundaries physically separate developing tissues into distinct regions, which is fundamental for the organisation of the body plan in both insects and vertebrates. In many examples, this physical segregation is caused by a regulated increase in contractility of the actomyosin cortex at boundary cell-cell interfaces, a property important in developmental morphogenesis beyond compartmental boundary formation. We performed an unbiased screening approach to identify cell surface receptors required for actomyosin enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, from the start of germband extension at gastrulation and throughout the germband extended stages (stages 6 to 11). First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs, confirming an earlier report. Next, by following in real time the dynamics of loss of boundary straightness in tartan mutant embryos compared with wild-type and ftz mutant embryos, we show that Tartan is required during germband extension but not beyond. We identify candidate genes that could take over from Tartan at PSBs and confirm that at germband extended stages, actomyosin enrichment at PSBs requires Wingless signalling. The Company of Biologists Ltd 2022-10-31 /pmc/articles/PMC9687003/ /pubmed/36178136 http://dx.doi.org/10.1242/dev.200292 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Sharrock, Thomas E.
Evans, Jenny
Blanchard, Guy B.
Sanson, Bénédicte
Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title_full Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title_fullStr Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title_full_unstemmed Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title_short Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
title_sort different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687003/
https://www.ncbi.nlm.nih.gov/pubmed/36178136
http://dx.doi.org/10.1242/dev.200292
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