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Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo

Lupus nephritis (LN) is a common and serious symptom in patients with systemic lupus erythematosus (SLE). Tubular interstitial fibrosis is a common underlying mechanism in the development of lupus nephritis to end-stage renal failure (ESRD). Quercetin is widely proven to prevent tissue fibrosis. The...

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Autores principales: Chen, Hsin-Yuan, Chiang, Yi-Fen, Hong, Yong-Han, Shieh, Tzong-Ming, Huang, Tsui-Chin, Ali, Mohamed, Chang, Hsin-Yi, Wang, Kai-Lee, Hsia, Shih-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687047/
https://www.ncbi.nlm.nih.gov/pubmed/36421424
http://dx.doi.org/10.3390/antiox11112238
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author Chen, Hsin-Yuan
Chiang, Yi-Fen
Hong, Yong-Han
Shieh, Tzong-Ming
Huang, Tsui-Chin
Ali, Mohamed
Chang, Hsin-Yi
Wang, Kai-Lee
Hsia, Shih-Min
author_facet Chen, Hsin-Yuan
Chiang, Yi-Fen
Hong, Yong-Han
Shieh, Tzong-Ming
Huang, Tsui-Chin
Ali, Mohamed
Chang, Hsin-Yi
Wang, Kai-Lee
Hsia, Shih-Min
author_sort Chen, Hsin-Yuan
collection PubMed
description Lupus nephritis (LN) is a common and serious symptom in patients with systemic lupus erythematosus (SLE). Tubular interstitial fibrosis is a common underlying mechanism in the development of lupus nephritis to end-stage renal failure (ESRD). Quercetin is widely proven to prevent tissue fibrosis. Therefore, the purpose of this study is to investigate the beneficial effects of quercetin on the inhibition of fibrosis and inflammation pathways in in vitro and in vivo lupus nephritis models. In the current study, MRL/lpr mice as animal models, and HK-2 human renal tubular epithelial cells were stimulated by interleukin-33 (IL-33) to mimic the cellular model of lupus nephritis. Immunohistochemical staining, immunoblotting assay, immunofluorescence staining, and quantitative real-time polymerase chain reaction assay were used. The in vivo results showed that quercetin improved the renal function and inhibited both fibrosis- and inflammation-related markers in MRL/lpr mice animal models. The in vitro results indicated that quercetin ameliorated the accumulation of fibrosis- and inflammation-related proteins in IL-33-induced HK-2 cells and improved renal cell pyroptosis via the IL33/ST2 pathway. Overall, quercetin can improve LN-related renal fibrosis and inflammation, which may offer an effective potential therapeutic strategy for lupus nephritis.
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spelling pubmed-96870472022-11-25 Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo Chen, Hsin-Yuan Chiang, Yi-Fen Hong, Yong-Han Shieh, Tzong-Ming Huang, Tsui-Chin Ali, Mohamed Chang, Hsin-Yi Wang, Kai-Lee Hsia, Shih-Min Antioxidants (Basel) Article Lupus nephritis (LN) is a common and serious symptom in patients with systemic lupus erythematosus (SLE). Tubular interstitial fibrosis is a common underlying mechanism in the development of lupus nephritis to end-stage renal failure (ESRD). Quercetin is widely proven to prevent tissue fibrosis. Therefore, the purpose of this study is to investigate the beneficial effects of quercetin on the inhibition of fibrosis and inflammation pathways in in vitro and in vivo lupus nephritis models. In the current study, MRL/lpr mice as animal models, and HK-2 human renal tubular epithelial cells were stimulated by interleukin-33 (IL-33) to mimic the cellular model of lupus nephritis. Immunohistochemical staining, immunoblotting assay, immunofluorescence staining, and quantitative real-time polymerase chain reaction assay were used. The in vivo results showed that quercetin improved the renal function and inhibited both fibrosis- and inflammation-related markers in MRL/lpr mice animal models. The in vitro results indicated that quercetin ameliorated the accumulation of fibrosis- and inflammation-related proteins in IL-33-induced HK-2 cells and improved renal cell pyroptosis via the IL33/ST2 pathway. Overall, quercetin can improve LN-related renal fibrosis and inflammation, which may offer an effective potential therapeutic strategy for lupus nephritis. MDPI 2022-11-13 /pmc/articles/PMC9687047/ /pubmed/36421424 http://dx.doi.org/10.3390/antiox11112238 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Hsin-Yuan
Chiang, Yi-Fen
Hong, Yong-Han
Shieh, Tzong-Ming
Huang, Tsui-Chin
Ali, Mohamed
Chang, Hsin-Yi
Wang, Kai-Lee
Hsia, Shih-Min
Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title_full Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title_fullStr Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title_full_unstemmed Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title_short Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo
title_sort quercetin ameliorates renal injury and pyroptosis in lupus nephritis through inhibiting il-33/st2 pathway in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687047/
https://www.ncbi.nlm.nih.gov/pubmed/36421424
http://dx.doi.org/10.3390/antiox11112238
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