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Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing
Precise genotyping is necessary to understand epidemiology and clinical manifestations of Chlamydia trachomatis infection with different genotypes. Next-generation high-throughput sequencing (NGHTS) has opened new frontiers in microbial genotyping, but has been clinically characterized in only a few...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687396/ https://www.ncbi.nlm.nih.gov/pubmed/36439830 http://dx.doi.org/10.3389/fmicb.2022.1041789 |
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author | Zhao, Jianhui Shui, Jingwei Luo, Lipei Ao, Cailing Lin, Hongqing Liang, Yuanhao Wang, Li Wang, Haiying Chen, Hongliang Tang, Shixing |
author_facet | Zhao, Jianhui Shui, Jingwei Luo, Lipei Ao, Cailing Lin, Hongqing Liang, Yuanhao Wang, Li Wang, Haiying Chen, Hongliang Tang, Shixing |
author_sort | Zhao, Jianhui |
collection | PubMed |
description | Precise genotyping is necessary to understand epidemiology and clinical manifestations of Chlamydia trachomatis infection with different genotypes. Next-generation high-throughput sequencing (NGHTS) has opened new frontiers in microbial genotyping, but has been clinically characterized in only a few settings. This study aimed to determine C. trachomatis genotypes in particular mixed-genotype infections and their association with clinical manifestations and to characterize the sensitivity and accuracy of NGHTS. Cervical specimens were collected from 8,087 subjects from physical examination center (PEC), assisted reproductive technology center (ART) and gynecology clinics (GC) of Chenzhou Hospital of China. The overall prevalence of C. trachomatis was 3.8% (311/8087) whereas a prevalence of 2.8, 3.7 and 4.8% was found in PEC, ART and GC, respectively. The most frequent three C. trachomatis genotypes were E (27.4%, 83/303), F (21.5%, 65/303) and J (18.2%, 55/303). Moreover, NGHTS identified 20 (6.6%, 20/303) mixed-genotype infections of C. trachomatis. Genotype G was more often observed in the subjects with pelvic inflammatory disease than genotype E (adjusted OR = 3.61, 95%CI, 1.02–12.8, p = 0.046). Mixed-genotype infection was associated with severe vaginal cleanliness (degree IV) with an adjusted OR of 5.17 (95%CI 1.03–25.9, p = 0.046) whereas mixed-genotype infection with large proportion of minor genotypes was associated with cervical squamous intraepithelial lesion (SIL) with an adjusted OR of 5.51 (95%CI 1.17–26.01, p = 0.031). Our results indicated that NGHTS is a feasible tool to identity C. trachomatis mixed-genotype infections, which may be associated with worse vaginal cleanliness and cervical SIL. |
format | Online Article Text |
id | pubmed-9687396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96873962022-11-25 Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing Zhao, Jianhui Shui, Jingwei Luo, Lipei Ao, Cailing Lin, Hongqing Liang, Yuanhao Wang, Li Wang, Haiying Chen, Hongliang Tang, Shixing Front Microbiol Microbiology Precise genotyping is necessary to understand epidemiology and clinical manifestations of Chlamydia trachomatis infection with different genotypes. Next-generation high-throughput sequencing (NGHTS) has opened new frontiers in microbial genotyping, but has been clinically characterized in only a few settings. This study aimed to determine C. trachomatis genotypes in particular mixed-genotype infections and their association with clinical manifestations and to characterize the sensitivity and accuracy of NGHTS. Cervical specimens were collected from 8,087 subjects from physical examination center (PEC), assisted reproductive technology center (ART) and gynecology clinics (GC) of Chenzhou Hospital of China. The overall prevalence of C. trachomatis was 3.8% (311/8087) whereas a prevalence of 2.8, 3.7 and 4.8% was found in PEC, ART and GC, respectively. The most frequent three C. trachomatis genotypes were E (27.4%, 83/303), F (21.5%, 65/303) and J (18.2%, 55/303). Moreover, NGHTS identified 20 (6.6%, 20/303) mixed-genotype infections of C. trachomatis. Genotype G was more often observed in the subjects with pelvic inflammatory disease than genotype E (adjusted OR = 3.61, 95%CI, 1.02–12.8, p = 0.046). Mixed-genotype infection was associated with severe vaginal cleanliness (degree IV) with an adjusted OR of 5.17 (95%CI 1.03–25.9, p = 0.046) whereas mixed-genotype infection with large proportion of minor genotypes was associated with cervical squamous intraepithelial lesion (SIL) with an adjusted OR of 5.51 (95%CI 1.17–26.01, p = 0.031). Our results indicated that NGHTS is a feasible tool to identity C. trachomatis mixed-genotype infections, which may be associated with worse vaginal cleanliness and cervical SIL. Frontiers Media S.A. 2022-11-10 /pmc/articles/PMC9687396/ /pubmed/36439830 http://dx.doi.org/10.3389/fmicb.2022.1041789 Text en Copyright © 2022 Zhao, Shui, Luo, Ao, Lin, Liang, Wang, Wang, Chen and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhao, Jianhui Shui, Jingwei Luo, Lipei Ao, Cailing Lin, Hongqing Liang, Yuanhao Wang, Li Wang, Haiying Chen, Hongliang Tang, Shixing Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title | Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title_full | Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title_fullStr | Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title_full_unstemmed | Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title_short | Identification and characterization of mixed infections of Chlamydia trachomatis via high-throughput sequencing |
title_sort | identification and characterization of mixed infections of chlamydia trachomatis via high-throughput sequencing |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687396/ https://www.ncbi.nlm.nih.gov/pubmed/36439830 http://dx.doi.org/10.3389/fmicb.2022.1041789 |
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