Cargando…

Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells

Pluripotent stem cells (PSCs) are important for future regenerative medicine therapies. However, in the production of PSCs and derivatives, the control of culture-induced fluctuations in the outcome of cell quality remains challenging. A detailed mechanistic understanding of how PSC behaviors are al...

Descripción completa

Detalles Bibliográficos
Autores principales: Thanuthanakhun, Naruchit, Kim, Mee-Hae, Kino-oka, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687444/
https://www.ncbi.nlm.nih.gov/pubmed/36354580
http://dx.doi.org/10.3390/bioengineering9110669
_version_ 1784836006631964672
author Thanuthanakhun, Naruchit
Kim, Mee-Hae
Kino-oka, Masahiro
author_facet Thanuthanakhun, Naruchit
Kim, Mee-Hae
Kino-oka, Masahiro
author_sort Thanuthanakhun, Naruchit
collection PubMed
description Pluripotent stem cells (PSCs) are important for future regenerative medicine therapies. However, in the production of PSCs and derivatives, the control of culture-induced fluctuations in the outcome of cell quality remains challenging. A detailed mechanistic understanding of how PSC behaviors are altered in response to biomechanical microenvironments within a culture is necessary for rational bioprocessing optimization. In this review, we discuss recent insights into the role of cell behavioral and mechanical homeostasis in modulating the states and functions of PSCs during culture processes. We delineate promising ways to manipulate the culture variability through regulating cell behaviors using currently developed tools. Furthermore, we anticipate their potential implementation for designing a culture strategy based on the concept of Waddington’s epigenetic landscape that may provide a feasible solution for tuning the culture quality and stability in the bioprocessing space.
format Online
Article
Text
id pubmed-9687444
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96874442022-11-25 Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells Thanuthanakhun, Naruchit Kim, Mee-Hae Kino-oka, Masahiro Bioengineering (Basel) Review Pluripotent stem cells (PSCs) are important for future regenerative medicine therapies. However, in the production of PSCs and derivatives, the control of culture-induced fluctuations in the outcome of cell quality remains challenging. A detailed mechanistic understanding of how PSC behaviors are altered in response to biomechanical microenvironments within a culture is necessary for rational bioprocessing optimization. In this review, we discuss recent insights into the role of cell behavioral and mechanical homeostasis in modulating the states and functions of PSCs during culture processes. We delineate promising ways to manipulate the culture variability through regulating cell behaviors using currently developed tools. Furthermore, we anticipate their potential implementation for designing a culture strategy based on the concept of Waddington’s epigenetic landscape that may provide a feasible solution for tuning the culture quality and stability in the bioprocessing space. MDPI 2022-11-09 /pmc/articles/PMC9687444/ /pubmed/36354580 http://dx.doi.org/10.3390/bioengineering9110669 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Thanuthanakhun, Naruchit
Kim, Mee-Hae
Kino-oka, Masahiro
Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title_full Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title_fullStr Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title_full_unstemmed Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title_short Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells
title_sort cell behavioral dynamics as a cue in optimizing culture stabilization in the bioprocessing of pluripotent stem cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687444/
https://www.ncbi.nlm.nih.gov/pubmed/36354580
http://dx.doi.org/10.3390/bioengineering9110669
work_keys_str_mv AT thanuthanakhunnaruchit cellbehavioraldynamicsasacueinoptimizingculturestabilizationinthebioprocessingofpluripotentstemcells
AT kimmeehae cellbehavioraldynamicsasacueinoptimizingculturestabilizationinthebioprocessingofpluripotentstemcells
AT kinookamasahiro cellbehavioraldynamicsasacueinoptimizingculturestabilizationinthebioprocessingofpluripotentstemcells