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Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding

High-frequency electric field welding-induced tissue fusion has been explored as an advanced surgical method for intestinal anastomoses; however, intrinsic mechanisms remain unclear. The aim of this study was to investigate microcosmic changes of collagen within the fusion area, with various paramet...

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Autores principales: Zhu, Caihui, Yin, Li, Xu, Jianzhi, Yang, Xingjian, Wang, Hao, Xiang, Xiaowei, Liu, Haotian, Liu, Kefu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687556/
https://www.ncbi.nlm.nih.gov/pubmed/36421697
http://dx.doi.org/10.3390/biom12111683
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author Zhu, Caihui
Yin, Li
Xu, Jianzhi
Yang, Xingjian
Wang, Hao
Xiang, Xiaowei
Liu, Haotian
Liu, Kefu
author_facet Zhu, Caihui
Yin, Li
Xu, Jianzhi
Yang, Xingjian
Wang, Hao
Xiang, Xiaowei
Liu, Haotian
Liu, Kefu
author_sort Zhu, Caihui
collection PubMed
description High-frequency electric field welding-induced tissue fusion has been explored as an advanced surgical method for intestinal anastomoses; however, intrinsic mechanisms remain unclear. The aim of this study was to investigate microcosmic changes of collagen within the fusion area, with various parameters. Ex vivo small intestine was fused with mucosa–mucosa. Four levels of compressive pressure (100 kPa, 150 kPa, 200 kPa, 250 kPa) were applied for 10 s in order to fuse the colons under a power level of 140 W. Then, collagen fibers of the fusion area were examined by fibrillar collagen alignment and TEM. Three levels of power (90 W, 110 W, 140 W) and three levels of time (5 s, 10 s, 20 s) were applied in order to fuse colons at 250 kPa, and then collagen within the fusion area was examined by Raman spectroscopy. Fibrillar collagen alignment analysis showed that with the increase in compression pressure, alignment of the collagen in the fusion area gradually increased, and the arrangement of collagen fibers tended to be consistent, which was conducive to the adhesion of collagen fibers. TEM showed that pressure changed the distribution and morphology of collagen fibers. Raman spectroscopy showed that increased power and time within a certain range contributed to collagen cross linking. Peak positions of amide I band and amide III band changed. These results suggested that higher power and a longer amount of time resulted in a decrease in non-reducible cross links and an increase in reducible cross links. Compression pressure, power, and time can affect the state of collagen, but the mechanisms are different. Compressive pressure affected the state of collagen by changing its orientation; power and time denatured collagen by increasing temperature and improved the reducible cross linking of collagen to promote tissue fusion.
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spelling pubmed-96875562022-11-25 Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding Zhu, Caihui Yin, Li Xu, Jianzhi Yang, Xingjian Wang, Hao Xiang, Xiaowei Liu, Haotian Liu, Kefu Biomolecules Article High-frequency electric field welding-induced tissue fusion has been explored as an advanced surgical method for intestinal anastomoses; however, intrinsic mechanisms remain unclear. The aim of this study was to investigate microcosmic changes of collagen within the fusion area, with various parameters. Ex vivo small intestine was fused with mucosa–mucosa. Four levels of compressive pressure (100 kPa, 150 kPa, 200 kPa, 250 kPa) were applied for 10 s in order to fuse the colons under a power level of 140 W. Then, collagen fibers of the fusion area were examined by fibrillar collagen alignment and TEM. Three levels of power (90 W, 110 W, 140 W) and three levels of time (5 s, 10 s, 20 s) were applied in order to fuse colons at 250 kPa, and then collagen within the fusion area was examined by Raman spectroscopy. Fibrillar collagen alignment analysis showed that with the increase in compression pressure, alignment of the collagen in the fusion area gradually increased, and the arrangement of collagen fibers tended to be consistent, which was conducive to the adhesion of collagen fibers. TEM showed that pressure changed the distribution and morphology of collagen fibers. Raman spectroscopy showed that increased power and time within a certain range contributed to collagen cross linking. Peak positions of amide I band and amide III band changed. These results suggested that higher power and a longer amount of time resulted in a decrease in non-reducible cross links and an increase in reducible cross links. Compression pressure, power, and time can affect the state of collagen, but the mechanisms are different. Compressive pressure affected the state of collagen by changing its orientation; power and time denatured collagen by increasing temperature and improved the reducible cross linking of collagen to promote tissue fusion. MDPI 2022-11-13 /pmc/articles/PMC9687556/ /pubmed/36421697 http://dx.doi.org/10.3390/biom12111683 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Caihui
Yin, Li
Xu, Jianzhi
Yang, Xingjian
Wang, Hao
Xiang, Xiaowei
Liu, Haotian
Liu, Kefu
Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title_full Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title_fullStr Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title_full_unstemmed Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title_short Characteristics of Collagen Changes in Small Intestine Anastomoses Induced by High-Frequency Electric Field Welding
title_sort characteristics of collagen changes in small intestine anastomoses induced by high-frequency electric field welding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687556/
https://www.ncbi.nlm.nih.gov/pubmed/36421697
http://dx.doi.org/10.3390/biom12111683
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