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Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival
Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypotherm...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687565/ https://www.ncbi.nlm.nih.gov/pubmed/36358934 http://dx.doi.org/10.3390/biom12111584 |
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author | Omori, Keiko Gonzalez, Ignacio Nguyen, Cindy Raminani, Shanti N. Deleon, Victor M. Meza, Pedro Zamalloa, Jose Perez, Rachel G. Gonzalez, Nelson Komatsu, Hirotake Al-Abdullah, Ismail H. Wen, Xin |
author_facet | Omori, Keiko Gonzalez, Ignacio Nguyen, Cindy Raminani, Shanti N. Deleon, Victor M. Meza, Pedro Zamalloa, Jose Perez, Rachel G. Gonzalez, Nelson Komatsu, Hirotake Al-Abdullah, Ismail H. Wen, Xin |
author_sort | Omori, Keiko |
collection | PubMed |
description | Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic β-cells preservation were first investigated. As low as 500 µg/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 °C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary β-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation. |
format | Online Article Text |
id | pubmed-9687565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96875652022-11-25 Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival Omori, Keiko Gonzalez, Ignacio Nguyen, Cindy Raminani, Shanti N. Deleon, Victor M. Meza, Pedro Zamalloa, Jose Perez, Rachel G. Gonzalez, Nelson Komatsu, Hirotake Al-Abdullah, Ismail H. Wen, Xin Biomolecules Article Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic β-cells preservation were first investigated. As low as 500 µg/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 °C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary β-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation. MDPI 2022-10-28 /pmc/articles/PMC9687565/ /pubmed/36358934 http://dx.doi.org/10.3390/biom12111584 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Omori, Keiko Gonzalez, Ignacio Nguyen, Cindy Raminani, Shanti N. Deleon, Victor M. Meza, Pedro Zamalloa, Jose Perez, Rachel G. Gonzalez, Nelson Komatsu, Hirotake Al-Abdullah, Ismail H. Wen, Xin Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title | Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title_full | Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title_fullStr | Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title_full_unstemmed | Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title_short | Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival |
title_sort | submilligram level of beetle antifreeze proteins minimize cold-induced cell swelling and promote cell survival |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687565/ https://www.ncbi.nlm.nih.gov/pubmed/36358934 http://dx.doi.org/10.3390/biom12111584 |
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