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Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687730/ https://www.ncbi.nlm.nih.gov/pubmed/36421105 http://dx.doi.org/10.3390/bioengineering9110704 |
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author | Krasilnikova, Olga A. Baranovskii, Denis S. Yakimova, Anna O. Arguchinskaya, Nadezhda Kisel, Anastas Sosin, Dmitry Sulina, Yana Ivanov, Sergey A. Shegay, Peter V. Kaprin, Andrey D. Klabukov, Ilya D. |
author_facet | Krasilnikova, Olga A. Baranovskii, Denis S. Yakimova, Anna O. Arguchinskaya, Nadezhda Kisel, Anastas Sosin, Dmitry Sulina, Yana Ivanov, Sergey A. Shegay, Peter V. Kaprin, Andrey D. Klabukov, Ilya D. |
author_sort | Krasilnikova, Olga A. |
collection | PubMed |
description | Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities. |
format | Online Article Text |
id | pubmed-9687730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96877302022-11-25 Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ Krasilnikova, Olga A. Baranovskii, Denis S. Yakimova, Anna O. Arguchinskaya, Nadezhda Kisel, Anastas Sosin, Dmitry Sulina, Yana Ivanov, Sergey A. Shegay, Peter V. Kaprin, Andrey D. Klabukov, Ilya D. Bioengineering (Basel) Review Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities. MDPI 2022-11-17 /pmc/articles/PMC9687730/ /pubmed/36421105 http://dx.doi.org/10.3390/bioengineering9110704 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Krasilnikova, Olga A. Baranovskii, Denis S. Yakimova, Anna O. Arguchinskaya, Nadezhda Kisel, Anastas Sosin, Dmitry Sulina, Yana Ivanov, Sergey A. Shegay, Peter V. Kaprin, Andrey D. Klabukov, Ilya D. Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title | Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title_full | Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title_fullStr | Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title_full_unstemmed | Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title_short | Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ |
title_sort | intraoperative creation of tissue-engineered grafts with minimally manipulated cells: new concept of bone tissue engineering in situ |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687730/ https://www.ncbi.nlm.nih.gov/pubmed/36421105 http://dx.doi.org/10.3390/bioengineering9110704 |
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