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Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for...

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Autores principales: Krasilnikova, Olga A., Baranovskii, Denis S., Yakimova, Anna O., Arguchinskaya, Nadezhda, Kisel, Anastas, Sosin, Dmitry, Sulina, Yana, Ivanov, Sergey A., Shegay, Peter V., Kaprin, Andrey D., Klabukov, Ilya D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687730/
https://www.ncbi.nlm.nih.gov/pubmed/36421105
http://dx.doi.org/10.3390/bioengineering9110704
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author Krasilnikova, Olga A.
Baranovskii, Denis S.
Yakimova, Anna O.
Arguchinskaya, Nadezhda
Kisel, Anastas
Sosin, Dmitry
Sulina, Yana
Ivanov, Sergey A.
Shegay, Peter V.
Kaprin, Andrey D.
Klabukov, Ilya D.
author_facet Krasilnikova, Olga A.
Baranovskii, Denis S.
Yakimova, Anna O.
Arguchinskaya, Nadezhda
Kisel, Anastas
Sosin, Dmitry
Sulina, Yana
Ivanov, Sergey A.
Shegay, Peter V.
Kaprin, Andrey D.
Klabukov, Ilya D.
author_sort Krasilnikova, Olga A.
collection PubMed
description Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities.
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spelling pubmed-96877302022-11-25 Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ Krasilnikova, Olga A. Baranovskii, Denis S. Yakimova, Anna O. Arguchinskaya, Nadezhda Kisel, Anastas Sosin, Dmitry Sulina, Yana Ivanov, Sergey A. Shegay, Peter V. Kaprin, Andrey D. Klabukov, Ilya D. Bioengineering (Basel) Review Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities. MDPI 2022-11-17 /pmc/articles/PMC9687730/ /pubmed/36421105 http://dx.doi.org/10.3390/bioengineering9110704 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Krasilnikova, Olga A.
Baranovskii, Denis S.
Yakimova, Anna O.
Arguchinskaya, Nadezhda
Kisel, Anastas
Sosin, Dmitry
Sulina, Yana
Ivanov, Sergey A.
Shegay, Peter V.
Kaprin, Andrey D.
Klabukov, Ilya D.
Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title_full Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title_fullStr Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title_full_unstemmed Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title_short Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ
title_sort intraoperative creation of tissue-engineered grafts with minimally manipulated cells: new concept of bone tissue engineering in situ
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687730/
https://www.ncbi.nlm.nih.gov/pubmed/36421105
http://dx.doi.org/10.3390/bioengineering9110704
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