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A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes

Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important li...

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Autores principales: Cuenca-Ortolá, Irene, Martínez-Rojas, Beatriz, Moreno-Manzano, Victoria, García Castelló, Marcos, Monleón Pradas, Manuel, Martínez-Ramos, Cristina, Más Estellés, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687775/
https://www.ncbi.nlm.nih.gov/pubmed/36359255
http://dx.doi.org/10.3390/biomedicines10112736
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author Cuenca-Ortolá, Irene
Martínez-Rojas, Beatriz
Moreno-Manzano, Victoria
García Castelló, Marcos
Monleón Pradas, Manuel
Martínez-Ramos, Cristina
Más Estellés, Jorge
author_facet Cuenca-Ortolá, Irene
Martínez-Rojas, Beatriz
Moreno-Manzano, Victoria
García Castelló, Marcos
Monleón Pradas, Manuel
Martínez-Ramos, Cristina
Más Estellés, Jorge
author_sort Cuenca-Ortolá, Irene
collection PubMed
description Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury.
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spelling pubmed-96877752022-11-25 A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes Cuenca-Ortolá, Irene Martínez-Rojas, Beatriz Moreno-Manzano, Victoria García Castelló, Marcos Monleón Pradas, Manuel Martínez-Ramos, Cristina Más Estellés, Jorge Biomedicines Article Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury. MDPI 2022-10-28 /pmc/articles/PMC9687775/ /pubmed/36359255 http://dx.doi.org/10.3390/biomedicines10112736 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cuenca-Ortolá, Irene
Martínez-Rojas, Beatriz
Moreno-Manzano, Victoria
García Castelló, Marcos
Monleón Pradas, Manuel
Martínez-Ramos, Cristina
Más Estellés, Jorge
A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title_full A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title_fullStr A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title_full_unstemmed A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title_short A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
title_sort strategy for magnetic and electric stimulation to enhance proliferation and differentiation of npcs seeded over pla electrospun membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687775/
https://www.ncbi.nlm.nih.gov/pubmed/36359255
http://dx.doi.org/10.3390/biomedicines10112736
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