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Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy

Long-term, repeatable monitoring of the appearance and progress of Alzheimer’s disease (AD) in real time can be extremely beneficial to acquire highly reliable diagnostic insights, which is crucial for devising apt strategies towards effective AD treatment. Herein, we present an optimized innovative...

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Autores principales: Luo, Ziyi, Xu, Hao, Samanta, Soham, Zhang, Renlong, Luo, Guoquan, Wang, Yiming, Liu, Liwei, Weng, Xiaoyu, He, Jun, Liao, Changrui, Wang, Yiping, Guo, Bingang, Qu, Junle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687891/
https://www.ncbi.nlm.nih.gov/pubmed/36428517
http://dx.doi.org/10.3390/biomedicines10112949
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author Luo, Ziyi
Xu, Hao
Samanta, Soham
Zhang, Renlong
Luo, Guoquan
Wang, Yiming
Liu, Liwei
Weng, Xiaoyu
He, Jun
Liao, Changrui
Wang, Yiping
Guo, Bingang
Qu, Junle
author_facet Luo, Ziyi
Xu, Hao
Samanta, Soham
Zhang, Renlong
Luo, Guoquan
Wang, Yiming
Liu, Liwei
Weng, Xiaoyu
He, Jun
Liao, Changrui
Wang, Yiping
Guo, Bingang
Qu, Junle
author_sort Luo, Ziyi
collection PubMed
description Long-term, repeatable monitoring of the appearance and progress of Alzheimer’s disease (AD) in real time can be extremely beneficial to acquire highly reliable diagnostic insights, which is crucial for devising apt strategies towards effective AD treatment. Herein, we present an optimized innovative cranial window imaging method for the long-term repeatable imaging of amyloid-β (Aβ) plaques and vessels in an AD mouse model. Basically, two-photon excitation fluorescence (TPEF) microscopy was used to monitor the fluorescently labeled Aβ plaques, whereas the label-free blood vessels were studied using coherent anti-Stokes Raman scattering (CARS) microscopy in the live in vivo AD mouse model. It was possible to clearly observe the Aβ deposition and vascular structure in the target cortex localization for 31 weeks in the AD mouse model using this method. The combined TPEF/CARS imaging studies were also instrumental in realizing the relationship between the tendency of Aβ deposition and ageing. Essentially, the progression of cerebral amyloid angiopathy (CAA) in the AD mouse model was quantitatively characterized, which revealed that the proportion Aβ deposition in the unit vessel can increase from 13.63% to 28.80% upon increasing the age of mice from 8 months old to 14 months old. The proposed imaging method provided an efficient, safe, repeatable platform with simple target localization aptitude towards monitoring the brain tissues, which is an integral part of studying any brain-related physiological or disease conditions to extract crucial structural and functional information.
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spelling pubmed-96878912022-11-25 Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy Luo, Ziyi Xu, Hao Samanta, Soham Zhang, Renlong Luo, Guoquan Wang, Yiming Liu, Liwei Weng, Xiaoyu He, Jun Liao, Changrui Wang, Yiping Guo, Bingang Qu, Junle Biomedicines Article Long-term, repeatable monitoring of the appearance and progress of Alzheimer’s disease (AD) in real time can be extremely beneficial to acquire highly reliable diagnostic insights, which is crucial for devising apt strategies towards effective AD treatment. Herein, we present an optimized innovative cranial window imaging method for the long-term repeatable imaging of amyloid-β (Aβ) plaques and vessels in an AD mouse model. Basically, two-photon excitation fluorescence (TPEF) microscopy was used to monitor the fluorescently labeled Aβ plaques, whereas the label-free blood vessels were studied using coherent anti-Stokes Raman scattering (CARS) microscopy in the live in vivo AD mouse model. It was possible to clearly observe the Aβ deposition and vascular structure in the target cortex localization for 31 weeks in the AD mouse model using this method. The combined TPEF/CARS imaging studies were also instrumental in realizing the relationship between the tendency of Aβ deposition and ageing. Essentially, the progression of cerebral amyloid angiopathy (CAA) in the AD mouse model was quantitatively characterized, which revealed that the proportion Aβ deposition in the unit vessel can increase from 13.63% to 28.80% upon increasing the age of mice from 8 months old to 14 months old. The proposed imaging method provided an efficient, safe, repeatable platform with simple target localization aptitude towards monitoring the brain tissues, which is an integral part of studying any brain-related physiological or disease conditions to extract crucial structural and functional information. MDPI 2022-11-16 /pmc/articles/PMC9687891/ /pubmed/36428517 http://dx.doi.org/10.3390/biomedicines10112949 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Ziyi
Xu, Hao
Samanta, Soham
Zhang, Renlong
Luo, Guoquan
Wang, Yiming
Liu, Liwei
Weng, Xiaoyu
He, Jun
Liao, Changrui
Wang, Yiping
Guo, Bingang
Qu, Junle
Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title_full Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title_fullStr Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title_full_unstemmed Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title_short Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy
title_sort long-term repeatable in vivo monitoring of amyloid-β plaques and vessels in alzheimer’s disease mouse model with combined tpef/cars microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687891/
https://www.ncbi.nlm.nih.gov/pubmed/36428517
http://dx.doi.org/10.3390/biomedicines10112949
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