Genetic Relationship, SPAD Reading, and Soluble Sugar Content as Indices for Evaluating the Graft Compatibility of Citrus Interstocks

SIMPLE SUMMARY: Grafting is a critical agricultural practice in citrus growing. The effectiveness of grafting not only depends on the technique but also on the stock–scion combinations. In this study, we investigated the grafting compatibility of five interstock combinations based on physiological and biochemical traits. The results revealed that the grafting compatibility in the early stages of grafting mediated by interstocks was related to the genetic relationship. The leaf chlorophyll content (SPAD reading, soil plant analysis development) and soluble sugar could be employed as preselected indicators to assess compatibility in the late stage of grafting. Our findings lay the foundation for the further research on rootstock–scion interaction mechanism. ABSTRACT: The interstock, a stock between the rootstock and scion, has a significant regulatory effect on the stock and scion, and its function is highly dependent on graft compatibility. To assess the graft compatibility of the interstock and scion, ‘Yuanxiaochun’ was top grafted onto ‘Ponkan’, ‘Shiranuhi’, ‘Harumi’, ‘Tarocco’, and ‘Kumquat’. The results showed significant differences in the survival ratio and preservation ratio among different combinations. Grafting compatibility in the early stages of grafting was associated with the genetic relationship. The biomass accumulation revealed that the interstock could influence both the rootstock and the scion. The physiological and biochemical traits analysis suggested that SPAD reading and soluble sugar could be employed as preselected indices to evaluate graft compatibility in the late stage of grafting. These results indicated that the evaluation of graft compatibility was a dynamic process. The findings provided a new approach for studying the stock and scion interaction mechanisms mediated by interstock, and directly provided a theoretical and practical basis for the high-grafting of ‘Yuanxiaochun’ citrus.