Cargando…

Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation

SIMPLE SUMMARY: Trichoderma spp. are common soil microorganisms that play an important role in limiting phytopathogenic microorganisms, improving plant growth and degrading plant biomass. Often the determining factors affecting the growth and maintenance of viability of Trichoderma spp. are the comp...

Descripción completa

Detalles Bibliográficos
Autores principales: Senkovs, Maris, Dzierkale, Marija Tereze, Rimkus, Alina, Grigs, Oskars, Nikolajeva, Vizma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687988/
https://www.ncbi.nlm.nih.gov/pubmed/36358311
http://dx.doi.org/10.3390/biology11111610
_version_ 1784836152553897984
author Senkovs, Maris
Dzierkale, Marija Tereze
Rimkus, Alina
Grigs, Oskars
Nikolajeva, Vizma
author_facet Senkovs, Maris
Dzierkale, Marija Tereze
Rimkus, Alina
Grigs, Oskars
Nikolajeva, Vizma
author_sort Senkovs, Maris
collection PubMed
description SIMPLE SUMMARY: Trichoderma spp. are common soil microorganisms that play an important role in limiting phytopathogenic microorganisms, improving plant growth and degrading plant biomass. Often the determining factors affecting the growth and maintenance of viability of Trichoderma spp. are the composition and condition of the growth medium. This study provides information on post-treatment procedures that would improve the viability of T. asperellum biomass and the antifungal activity obtained from submerged cultivation in a bioreactor. The aim of the study was to determine the viability of fungal biomass and competitiveness against a phytopathogen after treatment with hydrochloric acid, copper (II) sulphate and starch, alone or in combination. ABSTRACT: T. asperellum MSCL 309 was used in the study. T. asperellum was grown in the stirred bioreactor under submerged cultivation. The resulting biomass was filtered to obtain a thick biomass. The viability and antifungal activity of T. asperellum biomass samples were determined simultaneously by studying the colonization of the malt extract agar medium surface and its competitiveness with the plant pathogenic fungus Fusarium graminearum using in vitro dual culture experiments. Treatment with starch, alone or in combination with copper (II) sulphate and/or hydrochloric acid did not significantly affect fungal viability compared to control. An important factor in maintaining viability was the addition of hydrochloric acid, which significantly increased the storage life of biomass. In all post-treatments, F. graminearum was overgrown with T. asperellum in seven days, and accordingly, the larger the area occupied by T. asperellum, the smaller the area of F. graminearum colonization. Viability and antifungal activity of T. asperellum persisted throughout the experiment, at least for eight weeks. All the post-treatment methods we studied improved the viability and antifungal activity of Trichoderma, at least in terms of the area of the colonized surface. For the development of long-term viable and active T. asperellum preparations, we recommend the use of acidification of T. asperellum biomass obtained by submerged fermentation.
format Online
Article
Text
id pubmed-9687988
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96879882022-11-25 Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation Senkovs, Maris Dzierkale, Marija Tereze Rimkus, Alina Grigs, Oskars Nikolajeva, Vizma Biology (Basel) Article SIMPLE SUMMARY: Trichoderma spp. are common soil microorganisms that play an important role in limiting phytopathogenic microorganisms, improving plant growth and degrading plant biomass. Often the determining factors affecting the growth and maintenance of viability of Trichoderma spp. are the composition and condition of the growth medium. This study provides information on post-treatment procedures that would improve the viability of T. asperellum biomass and the antifungal activity obtained from submerged cultivation in a bioreactor. The aim of the study was to determine the viability of fungal biomass and competitiveness against a phytopathogen after treatment with hydrochloric acid, copper (II) sulphate and starch, alone or in combination. ABSTRACT: T. asperellum MSCL 309 was used in the study. T. asperellum was grown in the stirred bioreactor under submerged cultivation. The resulting biomass was filtered to obtain a thick biomass. The viability and antifungal activity of T. asperellum biomass samples were determined simultaneously by studying the colonization of the malt extract agar medium surface and its competitiveness with the plant pathogenic fungus Fusarium graminearum using in vitro dual culture experiments. Treatment with starch, alone or in combination with copper (II) sulphate and/or hydrochloric acid did not significantly affect fungal viability compared to control. An important factor in maintaining viability was the addition of hydrochloric acid, which significantly increased the storage life of biomass. In all post-treatments, F. graminearum was overgrown with T. asperellum in seven days, and accordingly, the larger the area occupied by T. asperellum, the smaller the area of F. graminearum colonization. Viability and antifungal activity of T. asperellum persisted throughout the experiment, at least for eight weeks. All the post-treatment methods we studied improved the viability and antifungal activity of Trichoderma, at least in terms of the area of the colonized surface. For the development of long-term viable and active T. asperellum preparations, we recommend the use of acidification of T. asperellum biomass obtained by submerged fermentation. MDPI 2022-11-03 /pmc/articles/PMC9687988/ /pubmed/36358311 http://dx.doi.org/10.3390/biology11111610 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Senkovs, Maris
Dzierkale, Marija Tereze
Rimkus, Alina
Grigs, Oskars
Nikolajeva, Vizma
Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title_full Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title_fullStr Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title_full_unstemmed Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title_short Application of a Posttreatment to Improve the Viability and Antifungal Activity of Trichoderma asperellum Biomass Obtained in a Bioreactor during Submerged Cultivation
title_sort application of a posttreatment to improve the viability and antifungal activity of trichoderma asperellum biomass obtained in a bioreactor during submerged cultivation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9687988/
https://www.ncbi.nlm.nih.gov/pubmed/36358311
http://dx.doi.org/10.3390/biology11111610
work_keys_str_mv AT senkovsmaris applicationofaposttreatmenttoimprovetheviabilityandantifungalactivityoftrichodermaasperellumbiomassobtainedinabioreactorduringsubmergedcultivation
AT dzierkalemarijatereze applicationofaposttreatmenttoimprovetheviabilityandantifungalactivityoftrichodermaasperellumbiomassobtainedinabioreactorduringsubmergedcultivation
AT rimkusalina applicationofaposttreatmenttoimprovetheviabilityandantifungalactivityoftrichodermaasperellumbiomassobtainedinabioreactorduringsubmergedcultivation
AT grigsoskars applicationofaposttreatmenttoimprovetheviabilityandantifungalactivityoftrichodermaasperellumbiomassobtainedinabioreactorduringsubmergedcultivation
AT nikolajevavizma applicationofaposttreatmenttoimprovetheviabilityandantifungalactivityoftrichodermaasperellumbiomassobtainedinabioreactorduringsubmergedcultivation