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Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688070/ https://www.ncbi.nlm.nih.gov/pubmed/36428967 http://dx.doi.org/10.3390/cells11223537 |
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author | Perik-Zavodskii, Roman Perik-Zavodskaia, Olga Shevchenko, Julia Denisova, Vera Alrhmoun, Saleh Volynets, Marina Tereshchenko, Valeriy Zaitsev, Konstantin Sennikov, Sergey |
author_facet | Perik-Zavodskii, Roman Perik-Zavodskaia, Olga Shevchenko, Julia Denisova, Vera Alrhmoun, Saleh Volynets, Marina Tereshchenko, Valeriy Zaitsev, Konstantin Sennikov, Sergey |
author_sort | Perik-Zavodskii, Roman |
collection | PubMed |
description | Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied NECs in adult bone marrow, umbilical cord blood, and foetal liver parenchyma using single-cell RNA sequencing and found that: (1) all studied NECs expressed the same set of genes, which was enriched in “GO biological process” immunity-related terms; (2) early and late NECs had differential expression of the genes associated with immunosuppression, cell cycle progression, apoptosis, and glycolysis; (3) NECs from different tissues of origin had differential expression of the genes associated with immunosuppression. |
format | Online Article Text |
id | pubmed-9688070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96880702022-11-25 Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing Perik-Zavodskii, Roman Perik-Zavodskaia, Olga Shevchenko, Julia Denisova, Vera Alrhmoun, Saleh Volynets, Marina Tereshchenko, Valeriy Zaitsev, Konstantin Sennikov, Sergey Cells Article Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied NECs in adult bone marrow, umbilical cord blood, and foetal liver parenchyma using single-cell RNA sequencing and found that: (1) all studied NECs expressed the same set of genes, which was enriched in “GO biological process” immunity-related terms; (2) early and late NECs had differential expression of the genes associated with immunosuppression, cell cycle progression, apoptosis, and glycolysis; (3) NECs from different tissues of origin had differential expression of the genes associated with immunosuppression. MDPI 2022-11-09 /pmc/articles/PMC9688070/ /pubmed/36428967 http://dx.doi.org/10.3390/cells11223537 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Perik-Zavodskii, Roman Perik-Zavodskaia, Olga Shevchenko, Julia Denisova, Vera Alrhmoun, Saleh Volynets, Marina Tereshchenko, Valeriy Zaitsev, Konstantin Sennikov, Sergey Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title | Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title_full | Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title_fullStr | Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title_full_unstemmed | Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title_short | Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing |
title_sort | immune transcriptome study of human nucleated erythroid cells from different tissues by single-cell rna-sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688070/ https://www.ncbi.nlm.nih.gov/pubmed/36428967 http://dx.doi.org/10.3390/cells11223537 |
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