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Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing

Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied...

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Autores principales: Perik-Zavodskii, Roman, Perik-Zavodskaia, Olga, Shevchenko, Julia, Denisova, Vera, Alrhmoun, Saleh, Volynets, Marina, Tereshchenko, Valeriy, Zaitsev, Konstantin, Sennikov, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688070/
https://www.ncbi.nlm.nih.gov/pubmed/36428967
http://dx.doi.org/10.3390/cells11223537
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author Perik-Zavodskii, Roman
Perik-Zavodskaia, Olga
Shevchenko, Julia
Denisova, Vera
Alrhmoun, Saleh
Volynets, Marina
Tereshchenko, Valeriy
Zaitsev, Konstantin
Sennikov, Sergey
author_facet Perik-Zavodskii, Roman
Perik-Zavodskaia, Olga
Shevchenko, Julia
Denisova, Vera
Alrhmoun, Saleh
Volynets, Marina
Tereshchenko, Valeriy
Zaitsev, Konstantin
Sennikov, Sergey
author_sort Perik-Zavodskii, Roman
collection PubMed
description Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied NECs in adult bone marrow, umbilical cord blood, and foetal liver parenchyma using single-cell RNA sequencing and found that: (1) all studied NECs expressed the same set of genes, which was enriched in “GO biological process” immunity-related terms; (2) early and late NECs had differential expression of the genes associated with immunosuppression, cell cycle progression, apoptosis, and glycolysis; (3) NECs from different tissues of origin had differential expression of the genes associated with immunosuppression.
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spelling pubmed-96880702022-11-25 Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing Perik-Zavodskii, Roman Perik-Zavodskaia, Olga Shevchenko, Julia Denisova, Vera Alrhmoun, Saleh Volynets, Marina Tereshchenko, Valeriy Zaitsev, Konstantin Sennikov, Sergey Cells Article Nucleated erythroid cells (NECs) are the precursors of erythrocytes. They can be found in various hematopoietic tissues or in the blood. Recently, they have been shown to be active players in immunosuppression through the synthesis of arginase-2 and reactive oxygen species. In this work, we studied NECs in adult bone marrow, umbilical cord blood, and foetal liver parenchyma using single-cell RNA sequencing and found that: (1) all studied NECs expressed the same set of genes, which was enriched in “GO biological process” immunity-related terms; (2) early and late NECs had differential expression of the genes associated with immunosuppression, cell cycle progression, apoptosis, and glycolysis; (3) NECs from different tissues of origin had differential expression of the genes associated with immunosuppression. MDPI 2022-11-09 /pmc/articles/PMC9688070/ /pubmed/36428967 http://dx.doi.org/10.3390/cells11223537 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perik-Zavodskii, Roman
Perik-Zavodskaia, Olga
Shevchenko, Julia
Denisova, Vera
Alrhmoun, Saleh
Volynets, Marina
Tereshchenko, Valeriy
Zaitsev, Konstantin
Sennikov, Sergey
Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title_full Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title_fullStr Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title_full_unstemmed Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title_short Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-Sequencing
title_sort immune transcriptome study of human nucleated erythroid cells from different tissues by single-cell rna-sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688070/
https://www.ncbi.nlm.nih.gov/pubmed/36428967
http://dx.doi.org/10.3390/cells11223537
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